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Literature summary for 3.1.3.8 extracted from
- Two types of phytases (histidine acid phytase and beta-propeller phytase) in Serratia sp. TN49 from the gut of Batocera horsfieldi (Coleoptera) larvae (2011), Curr. Microbiol., 63, 408-415.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates, dependent on, best at 1 mM, required for thermal stability | Serratia sp. |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene encoding PhyB49 PCR-amplified from the genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the phytase as N-terminally His6-tagged protein in Escherichia coli strain BL21(DE3) | Serratia sp. |
| gene encoding PhyH49 PCR-amplified from the genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the phytase as N-terminally His6-tagged protein in Escherichia coli strain BL21(DE3) | Serratia sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Serratia sp. | |
| Cr3+ | - |
Serratia sp. | |
| EDTA | - |
Serratia sp. | |
| Fe3+ | - |
Serratia sp. | |
| Mg2+ | - |
Serratia sp. | |
| Mn2+ | - |
Serratia sp. | |
| Ni2+ | - |
Serratia sp. | |
| SDS | - |
Serratia sp. | |
| Zn2+ | - |
Serratia sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
phytate | pH 5.0, 37°C | Serratia sp. | |
| 0.67 | - |
phytate | pH 7.5, 37°C | Serratia sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, recombinant His6-tagged phytase PhyH49, SDS-PAGE | Serratia sp. |
| 66000 | - |
x * 66000, recombinant His6-tagged phytase PhyB49, SDS-PAGE | Serratia sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phytate + H2O | Serratia sp. | - |
? + phosphate | - |
? | |
| phytate + H2O | Serratia sp. TN49, ACCC 03909 | - |
? + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia sp. | G4WZU3 | acidic histidine acid phosphatase PhyH49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae | - |
| Serratia sp. | G4WZU4 | alkaline beta-propeller phytase PhyB49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae | - |
| Serratia sp. TN49, ACCC 03909 | G4WZU3 | acidic histidine acid phosphatase PhyH49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae | - |
| Serratia sp. TN49, ACCC 03909 | G4WZU4 | alkaline beta-propeller phytase PhyB49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged phytase PhyB49 from Escherichia coli strain BL21(DE3) | Serratia sp. |
| recombinant His6-tagged phytase PhyH49 from Escherichia coli strain BL21(DE3) | Serratia sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.23 | - |
recombinant alkaline beta-propeller phosphatase PhyH49, pH 7.5, 37°C, in a strain grown under phosphate limitation in the presence of phytate | Serratia sp. |
| 0.58 | - |
recombinant acidic histidine phosphatase PhyH49, pH 4.5, 37°C, in a strain grown under phosphate limitation in the presence of phytate | Serratia sp. |
| 9.5 | - |
purified recombinant alkaline beta-propeller phosphatase PhyH49, pH 7.5, 37°C, substrate phytate | Serratia sp. |
| 144 | - |
purified recombinant acidic histidine phosphatase PhyH49, pH 4.5, 37°C, substrate phytate | Serratia sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | phytase activity is determined using the ferrous sulfate-molybdenum blue method | Serratia sp. | ? | - |
? | |
| additional information | phytase activity is determined using the ferrous sulfate-molybdenum blue method | Serratia sp. TN49, ACCC 03909 | ? | - |
? | |
| phytate + H2O | - |
Serratia sp. | ? + phosphate | - |
? | |
| phytate + H2O | - |
Serratia sp. TN49, ACCC 03909 | ? + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, recombinant His6-tagged phytase PhyH49, SDS-PAGE | Serratia sp. |
| ? | x * 66000, recombinant His6-tagged phytase PhyB49, SDS-PAGE | Serratia sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acidic histidine acid phosphatase | - |
Serratia sp. |
| alkaline beta-propeller phytase | - |
Serratia sp. |
| beta-propeller phytase | - |
Serratia sp. |
| histidine acid phytase | - |
Serratia sp. |
| PhyB49 | - |
Serratia sp. |
| PhyH49 | - |
Serratia sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
recombinant alkaline beta-propeller phosphatase PhyB49 | Serratia sp. |
| 60 | - |
recombinant acidic histidine phosphatase PhyH49 | Serratia sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme, stable at | Serratia sp. |
| 50 | - |
the thermal stability of recombinant PhyB49 is affected by the concentration of Ca2+, the recombinant enzyme is stable at 50°C for 30 min at 5 mM Ca2+, but loses activity rapidly under the same conditions in presence of 1 mM Ca2+ | Serratia sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23.46 | - |
phytate | pH 7.5, 37°C | Serratia sp. | |
| 112.7 | - |
phytate | pH 5.0, 37°C | Serratia sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant acidic histidine phosphatase PhyH49 | Serratia sp. |
| 7.5 | 8 | recombinant alkaline beta-propeller phosphatase PhyB49 | Serratia sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the two types of phytases are complementary in phytate degradation over the pH range of pH 2.0-9.0 | Serratia sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | purified recombinant enzyme, stable at pH 5.0-8.0, retaining more than 70% of the initial activity at 37°C for 1 h | Serratia sp. |
| 7 | 10 | purified recombinant enzyme, stable in neutral to alkaline buffers, retaining more than 70% of its activity at pH 7.0-10.0 and 37°C for 1 h | Serratia sp. |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the two phytases, an acidic histidine acid phosphatase, PhyH49, and an alkaline beta-propeller phytase, PhyB49, share low identities with known phytases, 61% at most | Serratia sp. |
| additional information | two types of phytases in Serratia sp. TN49 | Serratia sp. |
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