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Literature summary for 3.1.3.8 extracted from

  • Ragon, M.; Aumelas, A.; Chemardin, P.; Galvez, S.; Moulin, G.; Boze, H.
    Complete hydrolysis of myo-inositol hexakisphosphate by a novel phytase from Debaryomyces castellii CBS 2923 (2008), Appl. Microbiol. Biotechnol., 78, 47-53.
    View publication on PubMed
Search for more literature for 3.1.3.8

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, 10% decreased activity, although calcium has no direct influence on enzymatic activity, it plays a crucial protective role, since in the absence of calcium, the denaturation of the enzyme is complete after 1 h at 66.5°C Debaryomyces castellii
Fe2+ 5 mM, 10% decreased activity Debaryomyces castellii
Mn2+ 5 mM, 71% decreased activity Debaryomyces castellii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.532
-
 myo-inositol hexakisphosphate in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37°C Debaryomyces castellii
2.27
-
 p-nitrophenyl phosphate in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37°C Debaryomyces castellii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 SDS-PAGE, after deglycosylation Debaryomyces castellii
52980
-
 mass spectrometry analysis, after deglycosylation Debaryomyces castellii
74460
-
 mass spectrometry analysis, with 28.8% glycosylation Debaryomyces castellii
77000
-
 SDS-PAGE, with 28.8% glycosylation Debaryomyces castellii

Organism

Organism UniProt Comment Textmining
Debaryomyces castellii
-
-
-
Debaryomyces castellii CBS 2923
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 28.8% glycosylation Debaryomyces castellii

Purification (Commentary)

Purification (Comment) Organism
HiPrep Phenyl FF column chromatography Debaryomyces castellii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
157.4
-
 purified extract, at pH 4.0 Debaryomyces castellii

Storage Stability

Storage Stability Organism
60°C, in water, 1 h, remains stable Debaryomyces castellii
66°C, in sodium acetate buffer (125 mM, pH 4.0), 1 h, remains stable Debaryomyces castellii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phosphoglycerate + H2O 95% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii glycerate + phosphate
-
 ?
3-phosphoglycerate + H2O 95% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 glycerate + phosphate
-
 ?
ADP + H2O 120% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii AMP + phosphate
-
 ?
alpha-glycerophosphate + H2O 78% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii glycerol + phosphate
-
 ?
AMP + H2O 50% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii adenosine + phosphate
-
 ?
AMP + H2O 50% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 adenosine + phosphate
-
 ?
ATP + H2O 133% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii ADP + phosphate
-
 ?
D-fructose 6-phosphate + H2O 35% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii D-fructose + phosphate
-
 ?
D-fructose 6-phosphate + H2O 35% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 D-fructose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 82% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii D-glucose + phosphate
-
 ?
inositol 1-monophosphate + H2O 58% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii inositol + phosphate
-
 ?
inositol 2-monophosphate + H2O 84% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii inositol + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O 100% activity Debaryomyces castellii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
p-nitrophenyl phosphate + H2O 135% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O 135% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 p-nitrophenol + phosphate
-
 ?
phosphoenolpyruvate + H2O 130% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii pyruvate + phosphate
-
 ?
phosphoenolpyruvate + H2O 130% activity compared to myo-inositol hexakisphosphate Debaryomyces castellii CBS 2923 pyruvate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
3-phytase
-
 Debaryomyces castellii
myo-inositol hexakisphosphate 3-phosphohydrolase
-
 Debaryomyces castellii
PhyA
-
 Debaryomyces castellii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
 Debaryomyces castellii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 70% loss of activity after 1 h at 70°C Debaryomyces castellii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44.2
-
 p-nitrophenyl phosphate in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C Debaryomyces castellii
51.2
-
 myo-inositol hexakisphosphate in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C Debaryomyces castellii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
 Debaryomyces castellii

pH Range

pH Minimum pH Maximum Comment Organism
2.5 6.5
-
 Debaryomyces castellii

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 no denaturation is observed for pH values between 5.0 and 7.0 after 67 days of storage at -20°C, whereas the phytase is denatured for pH values lower than 5.0, for pH values ranging from 3.0 to 7.0, the enzyme retains 80 to 100% of its activity after incubation at 40°C for 1 h Debaryomyces castellii