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Literature summary for 3.1.3.8 extracted from

  • Tian, Y.; Peng, R.; Xu, J.; Zhao, W.; Gao, F.; Fu, X.; Xiong, A.; Yao, Q.
    Mutations in two amino acids in phyI1s from Aspergillus niger 113 improve its phytase activity (2010), World J. Microbiol. Biotechnol., 26, 903-907.
    View publication on PubMed
Search for more literature for 3.1.3.8

Application

Application Comment Organism
analysis gain of function mutants of the enzyme can be instrumental for the structure-function study of the enzyme and for industrial application Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
gene phyI1s, DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type and mutant enzymes Aspergillus niger

Protein Variants

Protein Variants Comment Organism
E121F random mutagenesis, the mutant shows 3.1fold increased activity and 3.24fold reduced affinity for sodium phytate compared to the wild-type enzyme Aspergillus niger
K41E random mutagenesis, the mutant shows 2.5fold increased activity and 1.78fold reduced affinity for sodium phytate compared to the wild-type enzyme Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.98
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant E121F, pH 5.0, 37°C Aspergillus niger
3.024
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant K41E, pH 5.0, 37°C Aspergillus niger
8.442
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Aspergillus niger
-
 ? + phosphate
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Aspergillus niger 113
-
 ? + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 gene phyI1s
-
Aspergillus niger 113
-
 gene phyI1s
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged wild-type and mutant enzymes by nickel affinity chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.82
-
 purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C Aspergillus niger
22.1
-
 purified recombinant His-tagged mutant K41E, pH 5.0, 37°C Aspergillus niger
27.28
-
 purified recombinant His-tagged mutant E121F, pH 5.0, 37°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Aspergillus niger ? + phosphate
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Aspergillus niger 113 ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
More structural model of the wild type and mutant enzymes, overview Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
59.83
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant E121F, pH 5.0, 37°C Aspergillus niger
83.67
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant K41E, pH 5.0, 37°C Aspergillus niger
161
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
 optimum 1 Aspergillus niger
5
-
 optimum 2 Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19.17
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C Aspergillus niger
27.83
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant K41E, pH 5.0, 37°C Aspergillus niger
30.5
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant E121F, pH 5.0, 37°C Aspergillus niger