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16
1-L-myo-inositol 1,2,3,4,5-pentakisphosphate
-
pH 5.5, 37°C
16
1-L-myo-inositol 1,2,3,5,6-pentakisphosphate
-
pH 5.5, 37°C
2
1-naphthyl phosphate
-
-
6
2-naphthyl phosphate
-
-
5.33 - 10.42
4-methylumbelliferyl phosphate
11.8 - 358000
4-nitrophenyl phosphate
161.9
4-nitrophenylphosphate
-
37°C, pH 4.5
1.1
D-fructose 1,6-diphosphate
-
pH 5.5, 37°C
1.6
D-Fructose 1-phosphate
-
pH 5.5, 37°C
101
D-glucose 1-phosphate
2.9
D-glucose 6-phosphate
-
pH 5.5, 37°C
1.1
D-ribose 5-phosphate
-
pH 5.5, 37°C
10
fructose 1,6-diphosphate
-
-
6
fructose 6-phosphate
-
-
2
glucose 6-phosphate
-
-
4
Glycerol 2-phosphate
-
-
0.07 - 412000
myo-inositol hexakisphosphate
231 - 281
myo-inositol hexakisphosphate disodium salt
253 - 279
myo-inositol hexakisphosphate sodium salt
3 - 370
myo-inositol-1,2,3,4,5,6-hexakisphosphate
1.9 - 44.2
p-nitrophenyl phosphate
198
phosphoenolpyruvate
-
37°C, pH 4.5
additional information
additional information
-
5.33
4-methylumbelliferyl phosphate
recombinant wild-type enzyme, pH 6.6, 37°C
10.18
4-methylumbelliferyl phosphate
recombinant mutant T44V/K45E, pH 6.6, 37°C
10.42
4-methylumbelliferyl phosphate
recombinant mutant T44I/K45E, pH 6.6, 37°C
11.8
4-nitrophenyl phosphate
-
pH 5.5, 50°C
20
4-nitrophenyl phosphate
-
-
99
4-nitrophenyl phosphate
-
-
104
4-nitrophenyl phosphate
-
-
357500
4-nitrophenyl phosphate
-
pH 2.5
358000
4-nitrophenyl phosphate
-
pH 2.5
101
D-glucose 1-phosphate
-
pH 5.5, 37°C
101
D-glucose 1-phosphate
-
37°C, pH 4.5
0.07
myo-inositol hexakisphosphate
mutant D56A, pH 7.0, temperature not specified in the publication
0.1
myo-inositol hexakisphosphate
mutant D56L, pH 7.0, temperature not specified in the publication
0.422
myo-inositol hexakisphosphate
recombinant mutant Q368E/K432R, pH 5.5, 37°C
0.476
myo-inositol hexakisphosphate
recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C
0.496
myo-inositol hexakisphosphate
recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C
0.548
myo-inositol hexakisphosphate
recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C
0.553
myo-inositol hexakisphosphate
recombinant mutant Q172R/K432R, pH 5.5, 37°C
0.557
myo-inositol hexakisphosphate
recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C
0.576
myo-inositol hexakisphosphate
recombinant mutant Q172R, pH 5.5, 37°C
0.71
myo-inositol hexakisphosphate
recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C
0.93
myo-inositol hexakisphosphate
-
5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60°C
1.67
myo-inositol hexakisphosphate
pH 6.0, 40°C, recombinant His-tagged C-terminal domain
3 - 6
myo-inositol hexakisphosphate
mutant C264G, pH 6.0, 37°C
3
myo-inositol hexakisphosphate
pH 7.0, 65°C
3.34
myo-inositol hexakisphosphate
mutant D56E, pH 7.0, temperature not specified in the publication
8.33
myo-inositol hexakisphosphate
mutant D308A, pH 7.0, temperature not specified in the publication
8.85
myo-inositol hexakisphosphate
-
37°C, pH 4.5
15.55
myo-inositol hexakisphosphate
mutant D308E, pH 7.0, temperature not specified in the publication
16.55
myo-inositol hexakisphosphate
wild-type, pH 7.0, temperature not specified in the publication
20.39
myo-inositol hexakisphosphate
pH 6.0, 40°C, recombinant His-tagged full-length enzyme
21
myo-inositol hexakisphosphate
-
pH 5.5, 37°C
21
myo-inositol hexakisphosphate
-
37°C, pH 4.5
25.4
myo-inositol hexakisphosphate
pH 7.4, 37°C, presence of Ca2+
26.6
myo-inositol hexakisphosphate
-
pH 7.0
37.8
myo-inositol hexakisphosphate
native enzyme, pH 5.0, 60°C
46
myo-inositol hexakisphosphate
-
-
46
myo-inositol hexakisphosphate
-
pH 5.0, 58°C
51
myo-inositol hexakisphosphate
-
pH 5.5
51.2
myo-inositol hexakisphosphate
-
in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C
51.2
myo-inositol hexakisphosphate
recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C
59.2
myo-inositol hexakisphosphate
native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C
62
myo-inositol hexakisphosphate
mutant C215S, pH 5.0, 37°C
90
myo-inositol hexakisphosphate
mutant C435G, pH 2.5, 42°C
102
myo-inositol hexakisphosphate
-
103
myo-inositol hexakisphosphate
-
sodium salt, pH 5.5, 50°C
114
myo-inositol hexakisphosphate
-
-
117
myo-inositol hexakisphosphate
-
calcium salt, pH 5.5, 50°C
134
myo-inositol hexakisphosphate
-
potassium salt, pH 5.5, 50°C
170
myo-inositol hexakisphosphate
-
pH 3.5, 37°C
180
myo-inositol hexakisphosphate
-
-
220
myo-inositol hexakisphosphate
-
-
234
myo-inositol hexakisphosphate
mutant C31G, pH 5.5, 53°C
236.2
myo-inositol hexakisphosphate
pH 4.5, 50°C
300
myo-inositol hexakisphosphate
-
pH 5.0, 58°C, recombinant enzyme
348
myo-inositol hexakisphosphate
-
-
348
myo-inositol hexakisphosphate
-
native fungal enzyme
348
myo-inositol hexakisphosphate
-
pH 5.0, 58°C
348
myo-inositol hexakisphosphate
-
pH 5.0, 58°C, native enzyme
350
myo-inositol hexakisphosphate
-
-
350
myo-inositol hexakisphosphate
wild-type, pH 5.0, 58°C
360
myo-inositol hexakisphosphate
-
recombinant enzyme
398
myo-inositol hexakisphosphate
in 1 M sodium acetate buffer pH 5.5, at 37°C
720
myo-inositol hexakisphosphate
pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris
840
myo-inositol hexakisphosphate
-
pH 3.5, 37°C
908
myo-inositol hexakisphosphate
-
in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C
950
myo-inositol hexakisphosphate
-
pH 5.0, 37°C
1300
myo-inositol hexakisphosphate
calcium phytate, pH 5.0, 60°C
1640
myo-inositol hexakisphosphate
-
pH 2.5, 60°C
1837.8
myo-inositol hexakisphosphate
pH 4.5, 37°C, recombinant wild-type enzyme
2056
myo-inositol hexakisphosphate
-
in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C
2230
myo-inositol hexakisphosphate
pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli
2283
myo-inositol hexakisphosphate
pH 4.5, 37°C
2450.5
myo-inositol hexakisphosphate
pH 4.5, 37°C, recombinant mutant D24G/K70R/K111E/N121S
2510.3
myo-inositol hexakisphosphate
pH 4.5, 37°C, recombinant mutant D24G/K265N
2527.8
myo-inositol hexakisphosphate
pH 4.5, 37°C, recombinant mutant D24G
2531
myo-inositol hexakisphosphate
pH 4.5, 37°C
2660
myo-inositol hexakisphosphate
-
mutant Q258N, pH 4.5, 37°C
3058
myo-inositol hexakisphosphate
-
mutant Q258N/Q349N, pH 4.5, 37°C
3397
myo-inositol hexakisphosphate
-
wild-type, pH 4.5, 37°C
4965.7
myo-inositol hexakisphosphate
pH 7.0, 37°C, recombinant wild-type enzyme
5100
myo-inositol hexakisphosphate
recombinant enzyme, pH 5.0, 60°C
7367.6
myo-inositol hexakisphosphate
pH 7.0, 37°C, recombinant mutant D24G
7860
myo-inositol hexakisphosphate
pH 7.0, 37°C, recombinant mutant D24G/K70R/K111E/N121S
8430.1
myo-inositol hexakisphosphate
pH 7.0, 37°C, recombinant mutant D24G/K265N
284600
myo-inositol hexakisphosphate
KM873028
recombinant enzyme, pH 3.9, 37°C
300000
myo-inositol hexakisphosphate
-
pH 2.5, 55°C. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
375200
myo-inositol hexakisphosphate
KM873028
recombinant enzyme, pH 3.9, 52.5°C
411700
myo-inositol hexakisphosphate
-
pH 5.5
412000
myo-inositol hexakisphosphate
-
pH 5.5
231
myo-inositol hexakisphosphate disodium salt
pH 5.5, 50°C
281
myo-inositol hexakisphosphate disodium salt
pH 5.5, 50°C
253
myo-inositol hexakisphosphate sodium salt
pH 5.5, 50°C
279
myo-inositol hexakisphosphate sodium salt
pH 5.5, 50°C
3 - 6
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 6.0, 37°C, recombinant mutant C264G
4.17
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DII, pH 7.0, 35°C
5.92
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase 168PhyA, pH 7.0, 55°C
12.28
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase 168PhyA, pH 7.0, 55°C
27.72
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
dual domain phytase PhyH, pH 7.0, 35°C
44.94
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase PhyP, pH 7.0, 37°C
59.83
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant E121F, pH 5.0, 37°C
62
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 5.0, 37°C, recombinant mutant C215S
83.67
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant K41E, pH 5.0, 37°C
90
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 2.5, 42°C, recombinant mutant C435G
94.4
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase PhyP, pH 7.0, 37°C
161
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C
234
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 5.5, 53°C, recombinant mutant C31G
248
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme b, pH 5.0, 36°C
253
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme b2, pH 5.0, 36°C
260
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme a, pH 5.0, 36°C
270
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme b1, pH 5.0, 36°C
279
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme a1, pH 5.0, 36°C
350
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 5.0, 58°C, recombinant wild-type enzyme
370
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH not specified in the publication, temperature not specified in the publication
1.9
p-nitrophenyl phosphate
in 1 M sodium acetate buffer pH 5.5, at 37°C
8.2
p-nitrophenyl phosphate
-
37°C, pH 4.5
44.2
p-nitrophenyl phosphate
-
in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C
0.82
phytate
-
mutant enzyme K76R
23.46
phytate
pH 7.5, 37°C
27
phytate
-
mutant enzyme Y159H
112.7
phytate
pH 5.0, 37°C
117
phytate
-
mutant enzyme D55A
1260
phytate
-
pH 2.5, 55°C
2348 - 4470
phytate
pH 4.0, 55°C
5530
phytate
-
mutant enzyme E227A
5532
phytate
-
mutant enzyme E227A
11800
phytate
-
mutant enzyme R122K
11810
phytate
-
mutant enzyme R122K
12400
phytate
-
mutant enzyme D258A
12430
phytate
-
mutant enzyme D258A
29060
phytate
-
mutant enzyme R122E
29100
phytate
-
mutant enzyme R122E
59550
phytate
-
wild-type enzyme
59600
phytate
-
wild-type enzyme
additional information
additional information
-
-
-
additional information
additional information
-
turnover number of immobilized enzyme
-