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Literature summary for 3.1.3.8 extracted from

  • Zhang, G.Q.; Dong, X.F.; Wang, Z.H.; Zhang, Q.; Wang, H.X.; Tong, J.M.
    Purification, characterization, and cloning of a novel phytase with low pH optimum and strong proteolysis resistance from Aspergillus ficuum NTG-23 (2010), Biores. Technol., 101, 4125-4131.
    View publication on PubMed
Search for more literature for 3.1.3.8

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight stimulation Aspergillus ficuum
EDTA slight activation at 1-10 mM Aspergillus ficuum

Application

Application Comment Organism
agriculture the phytase has the potential to be useful as an animal feed supplement Aspergillus ficuum
nutrition the phytase has the potential to be useful as an animal feed supplement Aspergillus ficuum

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aspergillus ficuum

General Stability

General Stability Organism
enzyme shows strong resistance toward pepsin and trypsin Aspergillus ficuum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect: Ca2+, Mg2+, Mn2+, Zn2+ Aspergillus ficuum
additional information no activation or inhibition by cations K+, Ca2+, Mg2+, Mn2+, Zn2+, Cd2+, Cu2+, Pb2+, Hg2+, Fe3+, and Al3+ Aspergillus ficuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65500
-
 gel filtration Aspergillus ficuum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phytate + H2O Aspergillus ficuum
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus ficuum
-
-
-
Aspergillus ficuum NTG-23
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 71.5fold by anion exchange chromatography, cation exchange chromatography, and gel filtration Aspergillus ficuum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus ficuum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
 37°C, pH 2.5 Aspergillus ficuum
150.1
-
 purified native enzyme, pH 2.5, 37°C Aspergillus ficuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-glycerophosphate + H2O 127% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum glycerate + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 254% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 254% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 4-nitrophenol + phosphate
-
 ?
ADP + H2O 177% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate
-
 ?
AMP + H2O 173% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum adenosine + phosphate
-
 ?
AMP + H2O 173% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 adenosine + phosphate
-
 ?
ATP + H2O 256% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate
-
 ?
D-fructose 6-phosphate + H2O 228% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-fructose + phosphate
-
 ?
D-fructose 6-phosphate + H2O 228% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 D-fructose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 190% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 190% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 D-glucose + phosphate
-
 ?
additional information the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate Aspergillus ficuum ?
-
 ?
additional information the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate Aspergillus ficuum NTG-23 ?
-
 ?
myo-inositol hexakisphosphate + H2O sodium salt Aspergillus ficuum myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
phytate + H2O
-
 Aspergillus ficuum ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65500, SDS-PAGE Aspergillus ficuum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
67
-
-
 Aspergillus ficuum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 purified native enzyme, stable at 60°C, no obvious loss of activity upon incubation at 70°C for 20 min Aspergillus ficuum
70
-
 20 min, no loss of activity Aspergillus ficuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3
-
-
 Aspergillus ficuum

General Information

General Information Comment Organism
additional information the enzyme shows strong resistance toward pepsin and trypsin Aspergillus ficuum