Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
myo-inositol hexakisphosphate | 37°C, pH 7.0 | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required both for refolding and activity | Bacillus sp. (in: Bacteria) | |
additional information | enzyme refolded in presence of Cu2+, Fe2+, Mg2+, Co2+, Zn2+ or Mn2+, fails to show any enzyme activity even in the presence of Ca2+ in the reaction mix | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | A5A5J1 | - |
- |
Bacillus sp. (in: Bacteria) DECSR1 | A5A5J1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, from inclusion bodies | Bacillus sp. (in: Bacteria) |
Renatured (Comment) | Organism |
---|---|
Ca2+ is required for refolding. Enzyme refolded in the presence of one of the divalent cations, Cu2+, Fe2+, Mg2+, Co2+, Zn2+ or Mn2+, fails to show any enzyme activity even in the presence of Ca2+ in the reaction mix | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
37°C, pH 7.0 | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Bacillus sp. (in: Bacteria) | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus sp. (in: Bacteria) DECSR1 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | - |
Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Bacillus sp. (in: Bacteria) |