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Literature summary for 3.1.3.8 extracted from
- Cloning, overexpression, and characterization of a metagenome-derived phytase with optimal activity at low pH (2015), J. Microbiol. Biotechnol., 25, 930-935 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene appA_Gw, DNA and amino acid sequence determination and analysis, cloning from metagenome, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 93% inhibition at 1 mM, complete inhibition at 10 mM | uncultured bacterium |  |
| Ca2+ | activates at 1-5 mM by up to 20%, inhibits at 10-50 mM | uncultured bacterium | |
| Co2+ | 16% inhibition at 5 mM, 49% at 10 mM | uncultured bacterium | |
| Cu2+ | 43% inhibition at 5 mM | uncultured bacterium | |
| Fe2+ | 64% inhibition at 5 mM | uncultured bacterium | |
| Mg2+ | 40% inhibition at 5 mM | uncultured bacterium | |
| Mn2+ | activates at 1 mM by 15%, inhibits 16% at 5 mM, 49% at 10 mM, and 85% at 50 mM | uncultured bacterium | |
| Ni2+ | 23% inhibition at 1 mM, 39% inhibition at 10 mM | uncultured bacterium | |
| Zn2+ | activates at 1 mM by 13%, inhibits 93% at 10 mM | uncultured bacterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
myo-inositol hexakisphosphate | pH 2.0, 37°C, recombinant enzyme | uncultured bacterium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the phytase might be a secreted enzyme located in the extracellular or periplasmic space of the host, residues 1-25 from the N-terminus are predicted to be a putative signal peptide of this phytase | uncultured bacterium | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
about, gel filtration, recombinant His-tagged enzyme | uncultured bacterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | uncultured bacterium | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | - |
metagenome-derived phytase, metagenome derived from subsurface groundwater | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | uncultured bacterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 298 | - |
purified recombinant enzyme, pH 2.0, 37°C | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | lower activity with 4-nitrophenyl phosphate, no or poor activity with AMP, ADP, ATP, GTP, NADP+, glucose 1-phosphate, and glucose 6-phosphate | uncultured bacterium | ? | - |
? | |
| myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | uncultured bacterium | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| appA_Gw | - |
uncultured bacterium |
| More | cf. EC 3.1.3.26 | uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | activity range, profile overview | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified recombinant enzyme, pH 2.0, after 30 min inactivation, loss of 50% activity after 15 min | uncultured bacterium |
| 80 | - |
purified recombinant enzyme, pH 2.0, after 25 min inactivation, loss of 50% activity after 7 min | uncultured bacterium |
| 100 | - |
purified recombinant enzyme, pH 2.0, after 20 min inactivation, loss of 50% activity after 4 min | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
- |
uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 4.5 | activity range, profile overview | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the histidine acid phosphatase (HAP) family | uncultured bacterium |
| additional information | the phytase seems to possess improved adaptability to the low pH condition caused by the gastric acid in livestock and poultry stomachs | uncultured bacterium |
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