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3.1.3.8: 3-phytase

This is an abbreviated version!
For detailed information about 3-phytase, go to the full flat file.

Word Map on EC 3.1.3.8

Reaction

myo-inositol hexakisphosphate
+
H2O
=
1D-myo-inositol 1,2,4,5,6-pentakisphosphate
 +
phosphate

Synonyms

1-phytase, 168phyA, 3'-phytase, 3-phytase, 3-phytase A, 3-phytase B, acid phytase, acidic histidine acid phosphatase, Afp, agpP, AgpP phytase, alkaline beta-propeller phytase, alkaline phytase, Allzyme, Allzyme phytase, AppA, appA phytase, AppA2, AppA2 phytase, AppAS, appA_Gw, Aspergillus niger N25 phytase, ASR1, AtPAP15, beta-propeller phytase, BIFPSEUDO_03792, Blon_0263, BPP, cell-bound phytase, cysteine phytase, dual-domain BPP, experimental phytase SP 1002, HAP, HAP phytase, HAP-phytase, histidine acid phosphatase, histidine acid phosphatase phytase, histidine acid phytase, HvPAPhy_a, LlALP1, LlALP2, microbial phytase, MiPhyA, MOK1 phytase, More, MYCTH_2306203, myo-inositol 1,2,3,4,5,6-hexakisphosphate phosphohydrolase, myo-inositol hexakiphosphate phosphohydrolase, myo-inositol hexakis phosphohydrolase, myo-inositol hexakisphosphate 3-phosphohydrolase, myo-inositol hexakisphosphate hydrolase, myo-inositol hexakisphosphate phosphohydrolase, myo-inositol hexaphosphate phosphohydrolase, myo-inositol-hexakisphosphate phosphohydrolase, MYO-inositol-hexaphosphate 3-phosphohydrolase, myo-inositol-hexaphosphate phosphohydrolase, myo-inositolhexakisphosphate phosphohydrolase, Natuphos, neutral phytase, NLP_0515, PAP, PAP type I, PAPhy, PAPhys, PAPhy_a1.1, pGF11 phytase, pGP209 phytase, pH 2.5 optimum acid phosphatase, Phy, PHY US42, PHY US573, phy168, PhyA, PhyA phytase, PhyA115, PhyA2, PhyB, PhyB49, PhyC, PhyCm, PhyH, PhyH49, PhyK, phyL, PhyP, phyS, phytase, phytase A, phytase C, phytate 1-phosphatase, Phytate 3-phosphatase, phytate 6-phosphatase, PhyTX52, PJ3 phytase, PPHY, protease-resistant phytase, PSphy, purple acid phosphatase, RMPhy1, RO1, RO2, Ronozyme P, RPHY1, ruminant microbial phytase, St-Phy, StPhy, tfphyA, YeAPPA, YkAPPA, YmPh

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.8 3-phytase

Specific Activity

Specific Activity on EC 3.1.3.8 - 3-phytase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000017
-
about, crude enzyme, pH 2.5, 15°C
0.0000083
0.0000167
0.00002
-
about, crude enzyme, pH 4.8, 15°C
0.000025
-
crude enzyme, pH 3.2, 15°C
0.0000275
-
about, crude enzyme, pH 3.0, 15°C
0.000133
0.00015
-
crude enzyme, pH 2.5, 15°C
0.000183
-
crude enzyme, pH 2.5, 15°C
0.0524
purified native enzyme, pH 5.0, 60°C
0.23
recombinant alkaline beta-propeller phosphatase PhyH49, pH 7.5, 37°C, in a strain grown under phosphate limitation in the presence of phytate
0.25
recombinant wild-type enzyme, pH 6.6, 37°C
0.58
recombinant acidic histidine phosphatase PhyH49, pH 4.5, 37°C, in a strain grown under phosphate limitation in the presence of phytate
0.744
-
root exsudate, at 37°C
1.3
-
recombinant mutant E227S, pH 7.0, 70°C
1.4
purified recombinant deglycosylated enzyme, pH 7.5, 37°C
1.68
recombinant mutant T44I/K45E, pH 6.6, 37°C
1.71
purified recombinant His-tagged wild-type enzyme, pH 4.5, 37°C
1.79
recombinant mutant T44V/K45E, pH 6.6, 37°C
1.82
-
functional domain Phyd-DII, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 35°C
10
-
37°C, pH 4.5
101
crude extract, recombinant enzyme, at 37°C
1072
-
pH 5.0, 37°C
11
purified recombinant enzyme, pH 7.5, 37°C
114.6
-
supernantant, at 60°C
1190
purified recombinant enzyme, pH 5.0, 60°C
12.81
purified recombinant His-tagged mutant K265E, pH 7.0, 60°C
13.01
-
beta-propeller phytase 168PhyA, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 55°C
13.82
purified recombinant His-tagged wild-type enzyme, pH 7.0, 60°C
133.3
mutant T11A/G56E/L65F/Q144H/L151S, pH 5.5-6.0, 37°C
1350
-
native enzyme from Penicillium chrysogenum strain CCT 1273, pH 5.0, 50°C
136.6
mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D, pH 4.8, 37°C
14
pH 7.4, 37°C, presence of Ca2+
14.87
-
incomplete domain Phyd-DI fused to beta-propeller phytase 168PhyA, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 55°C
144
purified recombinant acidic histidine phosphatase PhyH49, pH 4.5, 37°C, substrate phytate
145.9
mutant T11A/G56E/L65F/Q144H/L151S, pH 5.5-6.0, 50°C
15.3
about, purified recombinant His-tagged wild-type PhyC, pH 7.0, 65°C
15.68
purified recombinant His-tagged mutant S51A, pH 7.0, 60°C
150
-
37°C, pH 2.5
150.1
-
purified native enzyme, pH 2.5, 37°C
150.4
mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D, pH 4.8, 50°C
157.4
-
purified extract, at pH 4.0
17.24
purified recombinant His-tagged mutant S51A/K265E, pH 7.0, 60°C
17.88
purified recombinant His-tagged mutant S197E, pH 7.0, 65°C
17.93
purified recombinant His-tagged mutant D24G, pH 7.0, 60°C
174.1
purified enzyme, pH 4.5, 50°C
179.5
-
recombinant enzyme in potato leaves
1792
-
purified recombinant enzyme, pH 2.5, 37°C
18.14
purified recombinant His-tagged mutant D24G/S51A, pH 7.0, 60°C
18.17
purified recombinant His-tagged mutant D24G/N121S, pH 7.0, 60°C
18.5
-
recombinant mutant P257R, pH 7.0, 70°C
18.88
purified recombinant His-tagged mutant D24G/K111E, pH 7.0, 60°C
182
after 1.8fold purification, recombinant enzyme, at 37°C
1844
-
purified recombinant enzyme, pH 2.5, 40°C
19.27
purified recombinant His-tagged mutant D24G/K265E, pH 7.0, 60°C
19.33
purified recombinant His-tagged mutant D24G/S51A/K265E, pH 7.0, 60°C
19.67
purified recombinant His-tagged mutant D24G/K70R, pH 7.0, 60°C
19.7
purified recombinant enzyme mutant D24G/K70R/K111E/N121S, expressed in Escherichia coli, pH 7.0, 60°C
19.72
purified recombinant His-tagged mutant D24G/K265N, pH 7.0, 60°C
19.73
purified recombinant His-tagged mutant D24G/K70R/K111E/N121S, pH 7.0, 60°C
1952
purified recombinant His-tagged enzyme, pH 5.0, 55°C
2.1
partially purified enzyme, pH 7.0, 65°C
20.3
recombinant enzyme mutant R79L, pH 4.5, 37°C
20.64
purified recombinant His-tagged mutant D148E, pH 7.0, 65°C
202.25
-
purified native enzyme, pH 5.0, 60°C
21.61
-
beta-propeller phytase PhyP, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 37°C
22.1
-
purified recombinant His-tagged mutant K41E, pH 5.0, 37°C
22.7
purified recombinant enzyme mutant D24G/K70R/K111E/N121S, expressed in Pichia pastoris, pH 7.0, 60°C
2285
-
purified enzyme, pH 4.5, 50°C
2344
pH 4.5, 37°C
2384.6
purified enzyme, pH 6.5, 25°C
2456
pH 4.5, 37°C
25
pH 7.5, 55°C
2615
recombinant enzyme mutant R79G, pH 4.5, 37°C
2656
-
pH 4.5, 55°C
27
native extracellular enzyme, pH 7.5, 70°C
27.28
-
purified recombinant His-tagged mutant E121F, pH 5.0, 37°C
28.7
purifed recombinant enzyme, pH 7.5, 60°C
2860
-
recombinant enzyme from Penicillium griseoroseum strain T73, pH 5.0, 50°C
286923
recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C
29.82
-
incomplete domain Phyd-DI fused to beta-propeller phytase PhyP, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 37°C
298
-
purified recombinant enzyme, pH 2.0, 37°C
2982
purified recombinant nonglycosylated enzyme expressed from Escherichia coli, pH 5.5, 37°C
3.28
purified recombinant wild-type enzyme, pH 4.5, 37°C
30.4
purified recombinant enzyme mutant D24G/K70R/K111E/N121S, expressed in Bacillus subtilis, pH 7.0, 60°C
303715
recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C
31.2
-
recombinant mutant E180N, pH 7.0, 70°C
3137
-
mutant Q258N/Q349N, pH 4.5, 37°C
32.2
-
recombinant wild-type enzyme, pH 7.0, 70°C
32.76
wild-type enzyme, pH 5.5-6.0, 37°C
33
-
purified isozyme RO2, pH 4.5, 40°C
35
purified recombinant domain C towards phytate, pH 8.5, 45°C
35.7
-
recombinant mutant S283R, pH 7.0, 70°C
36.26
-
crude enzyme, pH 5.0, 37°C
361.7
-
after 486fold purification, at 37°C
37.8
-
recombinant mutant E229V, pH 7.0, 70°C
4.43
-
dual domain PhyH, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 35°C
4175
KM873028
purified recombinant enzyme, pH 3.9, 52.5°C
428
-
pH 2.5, 37°C
438366
recombinant mutant Q172R/K432R, pH 5.5, 37°C
447.5
purified native enzyme, pH 5.5, 55°C
46
purified recombinant enzyme, pH 5.0, 60°C
466688
recombinant mutant Q172R, pH 5.5, 37°C
467242
recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C
478761
recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C
493308
recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C
498
-
phytase LP2
5.45
-
partially purified enzyme, pH 7.0-7.5, 50-55°C
5.7
-
recombinant mutant K179R, pH 7.0, 70°C
5.92
purified recombinant His-tagged enzyme, pH and temperature not specified in the publication, substrate alpha-D-glucose 1-phosphate
503
recombinant enzyme, at 37°C
503217
recombinant mutant Q368E/K432R, pH 5.5, 37°C
53.13
-
recombinant enzyme, at 37°C
539
-
phytase LP11
54
-
purified isozyme RO1, pH 4.5, 40°C
6.32
purified recombinant His-tagged wild-type enzyme, pH 7.0, 37°C
60.63
purified enzyme, pH 5.0, 50°C, substrate calcium phytate
607
-
phytase LP12
7.3
-
recombinant mutant K77R, pH 7.0, 70°C
7.5
-
recombinant mutant K177R/K179R, pH 7.0, 70°C
72.97
-
purified enzyme, pH 6.5, 40°C
79.3
-
purified enzyme, pH 7.0, 37°C
792
-
pH 5.0, 37°C
8.61
purified native enzyme, pH 7.0, 50°C
8.82
-
purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C
8.9
recombinant enzyme secreted from heterologously expressing Pichia pastoris, pH 5.5, 37°C
8000
purified recombinant enzyme, pH 5.0, 60°C
805.98
-
purified enzyme, pH 2.5, 60°C
823.3
-
after 7.2fold purification, at 60°C
86.1
-
purified native enzyme, pH 5.5, 50°C, substrate phytate
870
-
pH 6.5, 37°C
89.4
recombinant enzyme mutant R79S, pH 4.5, 37°C
9.5
purified recombinant alkaline beta-propeller phosphatase PhyH49, pH 7.5, 37°C, substrate phytate
967
purified recombinant glycosylated enzyme expressed from Pichia pastoris, pH 5.5, 37°C
additional information