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0.49
1-L-myo-inositol 1,2,3,4,5-pentakisphosphate
-
pH 5.5, 37°C
0.51
1-L-myo-inositol 1,2,3,5,6-pentakisphosphate
-
pH 5.5, 37°C
0.49 - 250
1-naphthyl phosphate
0.667 - 0.881
2-glycerophosphate
0.512 - 230
2-naphthyl phosphate
1.562 - 3.495
4-methylumbelliferyl phosphate
0.04 - 120
4-nitrophenyl phosphate
0.703
4-nitrophenylphosphate
-
37°C, pH 4.5
2.2
D-fructose 1,6-bisphosphate
-
pH 5.5, 37°C
0.14 - 1.1
D-fructose 1,6-diphosphate
1.9
D-Fructose 1-phosphate
-
pH 5.5, 37°C
0.478 - 0.676
D-fructose 6-phosphate
0.26 - 0.28
D-glucose 1-phosphate
0.27 - 2.3
D-glucose 6-phosphate
1.2 - 9.8
D-ribose 5-phosphate
0.17
di-sodium phenyl phosphate dihydrate
-
at 62°C, in 50 mM sodium acetate buffer (pH 5)
0.229 - 18
fructose 1,6-diphosphate
56
fructose 6-phosphate
-
-
20
glucose 6-phosphate
-
-
110
Glycerol 2-phosphate
-
-
2
inositol bisphosphate
-
-
2
inositol monophosphate
-
-
2
inositol pentaphosphate
-
-
2
inositol tetraphosphate
-
-
2
inositol triphosphate
-
-
0.0104 - 114.8
myo-inositol hexakisphosphate
0.14 - 0.15
myo-inositol hexakisphosphate disodium salt
0.08 - 0.16
myo-inositol hexakisphosphate sodium salt
0.035 - 8.442
myo-inositol-1,2,3,4,5,6-hexakisphosphate
0.74 - 11
O-phospho-L-serine
0.52 - 18.16
p-nitrophenyl phosphate
0.801
phosphoenolpyruvate
-
37°C, pH 4.5
0.776 - 0.915
pyridoxal phosphate
additional information
additional information
-
0.49 - 2
1-naphthyl phosphate
-
pH 5.0, 35°C, phytase LP2
0.516
1-naphthyl phosphate
-
pH 5.0, 35°C, phytase LP11
0.718
1-naphthyl phosphate
-
pH 5.0, 35°C, phytase LP12
1.6
1-naphthyl phosphate
-
-
3.8
1-naphthyl phosphate
-
pH 5.5, 50°C
250
1-naphthyl phosphate
-
-
0.667
2-glycerophosphate
-
pH 5.0, 35°C, phytase LP11
0.704
2-glycerophosphate
-
pH 5.0, 35°C, phytase LP2
0.881
2-glycerophosphate
-
pH 5.0, 35°C, phytase LP12
0.512
2-naphthyl phosphate
-
pH 5.0, 35°C, phytase LP2
0.634
2-naphthyl phosphate
-
pH 5.0, 35°C, phytase LP12
0.653
2-naphthyl phosphate
-
pH 5.0, 35°C, phytase LP11
230
2-naphthyl phosphate
-
-
1.562
4-methylumbelliferyl phosphate
recombinant mutant T44V/K45E, pH 6.6, 37°C
1.598
4-methylumbelliferyl phosphate
recombinant mutant T44I/K45E, pH 6.6, 37°C
3.495
4-methylumbelliferyl phosphate
recombinant wild-type enzyme, pH 6.6, 37°C
0.04
4-nitrophenyl phosphate
-
pH 5.0, 45°C
0.123
4-nitrophenyl phosphate
-
pH 5.0, 35°C, phytase LP12
0.152
4-nitrophenyl phosphate
-
pH 5.0, 35°C, phytase LP11
0.176
4-nitrophenyl phosphate
-
pH 5.0, 35°C, phytase LP2
0.265
4-nitrophenyl phosphate
-
-
0.93
4-nitrophenyl phosphate
-
pH 5.5, 50°C
1.38
4-nitrophenyl phosphate
-
-
1.38
4-nitrophenyl phosphate
-
pH 2.5
2.2
4-nitrophenyl phosphate
-
pH 5.6
4.3
4-nitrophenyl phosphate
-
-
10.1
4-nitrophenyl phosphate
-
pH 5.5, 50°C
120
4-nitrophenyl phosphate
-
-
0.398
ADP
-
pH 5.0, 35°C, phytase LP12
0.403
ADP
-
pH 5.0, 35°C, phytase LP11
0.451
ADP
-
pH 5.0, 35°C, phytase LP2
0.315
AMP
-
pH 5.0, 35°C, phytase LP12
0.319
AMP
-
pH 5.0, 35°C, phytase LP2
0.365
AMP
-
pH 5.0, 35°C, phytase LP11
0.07
ATP
-
at 62°C, in 50 mM sodium acetate buffer (pH 5)
0.412
ATP
-
pH 5.0, 35°C, phytase LP12
0.531
ATP
-
pH 5.0, 35°C, phytase LP11
0.596
ATP
-
pH 5.0, 35°C, phytase LP2
0.14
D-fructose 1,6-diphosphate
-
at 62°C, in 50 mM sodium acetate buffer (pH 5)
0.359
D-fructose 1,6-diphosphate
-
pH 5.0, 35°C, phytase LP2
0.731
D-fructose 1,6-diphosphate
-
pH 5.0, 35°C, phytase LP12
1.1
D-fructose 1,6-diphosphate
-
-
0.478
D-fructose 6-phosphate
-
pH 5.0, 35°C, phytase LP11
0.531
D-fructose 6-phosphate
-
pH 5.0, 35°C, phytase LP2
0.676
D-fructose 6-phosphate
-
pH 5.0, 35°C, phytase LP12
0.26
D-glucose 1-phosphate
-
pH 5.5, 37°C
0.26
D-glucose 1-phosphate
-
37°C, pH 4.5
0.28
D-glucose 1-phosphate
-
at 62°C, in 50 mM sodium acetate buffer (pH 5)
0.27
D-glucose 6-phosphate
-
at 62°C, in 50 mM sodium acetate buffer (pH 5)
0.302
D-glucose 6-phosphate
-
pH 5.0, 35°C, phytase LP2
0.401
D-glucose 6-phosphate
-
pH 5.0, 35°C, phytase LP11
0.634
D-glucose 6-phosphate
-
pH 5.0, 35°C, phytase LP12
1.3
D-glucose 6-phosphate
-
pH 5.5, 37°C
2.3
D-glucose 6-phosphate
-
-
1.2
D-ribose 5-phosphate
-
-
9.8
D-ribose 5-phosphate
-
pH 5.5, 37°C
0.445
diphosphate
-
pH 5.0, 35°C, phytase LP11
0.691
diphosphate
-
pH 5.0, 35°C, phytase LP12
0.812
diphosphate
-
pH 5.0, 35°C, phytase LP2
0.229
fructose 1,6-diphosphate
-
pH 5.0, 35°C, phytase LP11
18
fructose 1,6-diphosphate
-
-
0.217
GTP
-
pH 5.0, 35°C, phytase LP2
0.398
GTP
-
pH 5.0, 35°C, phytase LP11
0.423
GTP
-
pH 5.0, 35°C, phytase LP12
0.0104
myo-inositol hexakisphosphate
-
pH 5.5
0.01073
myo-inositol hexakisphosphate
recombinant mutant Q368E/K432R, pH 5.5, 37°C
0.0139
myo-inositol hexakisphosphate
-
in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C
0.0147
myo-inositol hexakisphosphate
-
in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C
0.01547
myo-inositol hexakisphosphate
recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C
0.016
myo-inositol hexakisphosphate
-
-
0.01794
myo-inositol hexakisphosphate
recombinant mutant Q172R, pH 5.5, 37°C
0.01927
myo-inositol hexakisphosphate
recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C
0.02075
myo-inositol hexakisphosphate
recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C
0.021
myo-inositol hexakisphosphate
-
at 62°C, in 50 mM sodium acetate buffer (pH 5)
0.02189
myo-inositol hexakisphosphate
recombinant mutant Q172R/K432R, pH 5.5, 37°C
0.027
myo-inositol hexakisphosphate
-
-
0.027
myo-inositol hexakisphosphate
-
native fungal enzyme
0.027
myo-inositol hexakisphosphate
-
pH 5.0, 58°C, native enzyme
0.02769
myo-inositol hexakisphosphate
recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C
0.03
myo-inositol hexakisphosphate
-
pH 5.0, 58°C
0.03073
myo-inositol hexakisphosphate
recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C
0.034
myo-inositol hexakisphosphate
-
pH 3.5, 37°C
0.038
myo-inositol hexakisphosphate
-
-
0.04
myo-inositol hexakisphosphate
-
-
0.04
myo-inositol hexakisphosphate
-
-
0.04
myo-inositol hexakisphosphate
Schwanniomyces castellii
-
-
0.04
myo-inositol hexakisphosphate
-
strain NRRL 3135
0.04
myo-inositol hexakisphosphate
strain NRRL 3135
0.04
myo-inositol hexakisphosphate
-
pH 5.0, 58°C
0.045
myo-inositol hexakisphosphate
mutant C435G, pH 2.5, 42°C
0.05
myo-inositol hexakisphosphate
-
pH 7.0
0.05
myo-inositol hexakisphosphate
-
at pH 7.5
0.05
myo-inositol hexakisphosphate
-
pH 5.0, 58°C, recombinant enzyme
0.056
myo-inositol hexakisphosphate
mutant C264G, pH 6.0, 37°C
0.06
myo-inositol hexakisphosphate
-
at pH 6.5
0.061
myo-inositol hexakisphosphate
-
-
0.062
myo-inositol hexakisphosphate
-
high molecular weight form
0.065
myo-inositol hexakisphosphate
-
recombinant enzyme
0.07
myo-inositol hexakisphosphate
wild-type, pH 5.0, 58°C
0.074
myo-inositol hexakisphosphate
-
pH 3.5, 37°C
0.078
myo-inositol hexakisphosphate
-
pH 4.5, 55°C
0.08
myo-inositol hexakisphosphate
-
pH 5.0, 35°C, phytase LP11
0.0827
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K, at pH 4.0
0.0864
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P, at pH 4.0
0.0934
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P/F131L/K112R/K195R, at pH 4.0
0.1
myo-inositol hexakisphosphate
-
-
0.1
myo-inositol hexakisphosphate
-
at pH 6
0.1
myo-inositol hexakisphosphate
-
at pH 5.3
0.1064
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R, at 37°C, in 0.2 M citrate buffer, pH 5.5
0.1076
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P, at pH 5.5
0.1083
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K, at pH 5.5
0.11
myo-inositol hexakisphosphate
-
-
0.114
myo-inositol hexakisphosphate
-
low molecular weight form
0.114
myo-inositol hexakisphosphate
in 200 mM sodium acetate buffer (pH 5.5), at 37°C
0.1224
myo-inositol hexakisphosphate
-
wild type enzyme, at pH 4.0
0.124
myo-inositol hexakisphosphate
-
pH 5.0, 58°C, recombinant enzyme
0.13
myo-inositol hexakisphosphate
-
-
0.13
myo-inositol hexakisphosphate
-
pH 5.0, 35°C, phytase LP2
0.1304
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S, at 37°C, in 0.2 M citrate buffer, pH 5.5
0.135
myo-inositol hexakisphosphate
-
-
0.135
myo-inositol hexakisphosphate
-
immobilized enzyme
0.1363
myo-inositol hexakisphosphate
wild-type, pH 9.0, temperature not specified in the publication
0.138
myo-inositol hexakisphosphate
wild-type, pH 7.0, temperature not specified in the publication
0.143
myo-inositol hexakisphosphate
wild-type, pH 8.0, temperature not specified in the publication
0.143
myo-inositol hexakisphosphate
recombinant enzyme, pH 5.0, 60°C
0.145
myo-inositol hexakisphosphate
mutant C215S, pH 5.0, 37°C
0.146
myo-inositol hexakisphosphate
wild-type, pH 6.0, temperature not specified in the publication
0.147
myo-inositol hexakisphosphate
calcium phytate, pH 5.0, 60°C
0.15
myo-inositol hexakisphosphate
-
-
0.15
myo-inositol hexakisphosphate
-
at pH 5.5
0.151
myo-inositol hexakisphosphate
wild-type, pH 5.0, temperature not specified in the publication
0.154
myo-inositol hexakisphosphate
-
mutant enzyme A58E/Q191R, at 37°C, in 0.2 M citrate buffer, pH 5.5
0.156
myo-inositol hexakisphosphate
mutant D308E, pH 7.0, temperature not specified in the publication
0.156
myo-inositol hexakisphosphate
native enzyme, pH 5.0, 60°C
0.156
myo-inositol hexakisphosphate
native enzyme, pH 5.0, 60°C
0.159
myo-inositol hexakisphosphate
wild-type, pH 4.0, temperature not specified in the publication
0.159
myo-inositol hexakisphosphate
-
substrate sodium phytate, pH 5.0, 60°C, recombinant enzyme
0.16
myo-inositol hexakisphosphate
-
pH 5.0
0.16
myo-inositol hexakisphosphate
-
pH 5.0, 50°C
0.1623
myo-inositol hexakisphosphate
-
mutant enzyme A58E/Q191R/T271R, at 37°C, in 0.2 M citrate buffer, pH 5.5
0.167
myo-inositol hexakisphosphate
mutant D308A, pH 7.0, temperature not specified in the publication
0.1675
myo-inositol hexakisphosphate
-
wild type enzyme, at 37°C, in 0.2 M citrate buffer, pH 5.5
0.17
myo-inositol hexakisphosphate
-
pH 5.0, 37°C
0.1719
myo-inositol hexakisphosphate
-
wild type enzyme, at pH 5.5
0.18
myo-inositol hexakisphosphate
mutant C31G, pH 5.5, 53°C
0.18
myo-inositol hexakisphosphate
pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli
0.189
myo-inositol hexakisphosphate
mutant D56E, pH 7.0, temperature not specified in the publication
0.196
myo-inositol hexakisphosphate
pH 4.5, 37°C
0.196
myo-inositol hexakisphosphate
in 1 M sodium acetate buffer pH 5.5, at 37°C
0.2
myo-inositol hexakisphosphate
-
-
0.2
myo-inositol hexakisphosphate
-
-
0.2
myo-inositol hexakisphosphate
-
at pH 2.5
0.206
myo-inositol hexakisphosphate
pH 4.5, 37°C
0.208
myo-inositol hexakisphosphate
-
substrate potassium phytate, pH 5.0, 60°C, recombinant enzyme
0.21
myo-inositol hexakisphosphate
-
pH 2.5, 60°C
0.218
myo-inositol hexakisphosphate
mutant D56L, pH 7.0, temperature not specified in the publication
0.22
myo-inositol hexakisphosphate
pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris
0.24
myo-inositol hexakisphosphate
native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C
0.247
myo-inositol hexakisphosphate
-
pH 5.0, 37°C
0.248
myo-inositol hexakisphosphate
mutant D56A, pH 7.0, temperature not specified in the publication
0.25
myo-inositol hexakisphosphate
-
-
0.25
myo-inositol hexakisphosphate
-
-
0.25
myo-inositol hexakisphosphate
-
-
0.252
myo-inositol hexakisphosphate
-
pH 5.0, 37°C
0.262
myo-inositol hexakisphosphate
KM873028
recombinant enzyme, pH 3.9, 52.5°C
0.278
myo-inositol hexakisphosphate
-
37°C, pH 4.5
0.28
myo-inositol hexakisphosphate
recombinant His-tagged enzyme, pH and temperature not specified in the publication
0.293
myo-inositol hexakisphosphate
pH 7.0, 50°C, sodium phytate
0.3
myo-inositol hexakisphosphate
-
-
0.3
myo-inositol hexakisphosphate
-
pH 5.0, 35°C, phytase LP12
0.3
myo-inositol hexakisphosphate
-
37°C, pH 3.5, enzyme expressed in Schizosaccharomyces pombe. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
0.32
myo-inositol hexakisphosphate
-
sodium phytate, pH 5.0, 60°C
0.326
myo-inositol hexakisphosphate
KM873028
recombinant enzyme, pH 3.9, 37°C
0.33
myo-inositol hexakisphosphate
-
-
0.34
myo-inositol hexakisphosphate
-
0.34
myo-inositol hexakisphosphate
-
pH 5.5, 37°C
0.34
myo-inositol hexakisphosphate
-
37°C, pH 4.5
0.35
myo-inositol hexakisphosphate
-
-
0.36
myo-inositol hexakisphosphate
pH 7.4, 37°C, presence of Ca2+
0.38
myo-inositol hexakisphosphate
-
pH 5.5, 40°C
0.382
myo-inositol hexakisphosphate
-
recombinant wild-type enzyme, pH 2.5, 40°C
0.3847
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P/F131L/K112R/K195R, at pH 5.5
0.39
myo-inositol hexakisphosphate
-
-
0.39
myo-inositol hexakisphosphate
37°C, pH 7.0
0.39
myo-inositol hexakisphosphate
pH 4.5, 37°C, recombinant mutant D24G/K70R/K111E/N121S
0.405
myo-inositol hexakisphosphate
-
recombinant mutant enzyme, pH 2.5, 40°C
0.42
myo-inositol hexakisphosphate
pH 4.5, 50°C
0.43
myo-inositol hexakisphosphate
-
mutant Q258N/Q349N, pH 4.5, 37°C
0.44
myo-inositol hexakisphosphate
-
-
0.44
myo-inositol hexakisphosphate
-
strain 92
0.44
myo-inositol hexakisphosphate
strain 92
0.45
myo-inositol hexakisphosphate
-
-
0.46
myo-inositol hexakisphosphate
-
pH 4, 37°C
0.48
myo-inositol hexakisphosphate
-
strain IIIAn/8
0.48
myo-inositol hexakisphosphate
-
wild-type, pH 4.5, 37°C
0.48 - 0.545
myo-inositol hexakisphosphate
pH 5.5, 55°C, sodium phytate
0.49
myo-inositol hexakisphosphate
pH 4.5, 37°C, recombinant mutant D24G/K265N
0.5
myo-inositol hexakisphosphate
-
Bacillus subtilis var. natto
0.51
myo-inositol hexakisphosphate
-
pH 2.0, 37°C, recombinant enzyme
0.52
myo-inositol hexakisphosphate
pH 7.5, 55°C
0.52
myo-inositol hexakisphosphate
-
mutant Q258N, pH 4.5, 37°C
0.532
myo-inositol hexakisphosphate
-
in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37°C
0.532
myo-inositol hexakisphosphate
recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C
0.54
myo-inositol hexakisphosphate
pH 4.5, 37°C, recombinant mutant D24G
0.545
myo-inositol hexakisphosphate
pH 4.5, 55°C
0.55
myo-inositol hexakisphosphate
-
-
0.58
myo-inositol hexakisphosphate
-
sodium salt, pH 5.5, 50°C
0.59
myo-inositol hexakisphosphate
-
calcium salt, pH 5.5, 50°C
0.606
myo-inositol hexakisphosphate
-
pH 2.5, 55°C. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
0.63
myo-inositol hexakisphosphate
-
-
0.667
myo-inositol hexakisphosphate
-
substrate calcium phytate, pH 5.0, 60°C, recombinant enzyme
0.7
myo-inositol hexakisphosphate
-
37°C, pH 3.5, enzyme expressed in Pichia pastoris (pPICZalphaA). The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
0.72
myo-inositol hexakisphosphate
-
pH 4.5, 45°C
0.757
myo-inositol hexakisphosphate
pH 5.0, 55°C, recombinant His-tagged enzyme
0.78
myo-inositol hexakisphosphate
pH 4.5, 37°C, recombinant wild-type enzyme
0.8
myo-inositol hexakisphosphate
-
37°C, pH 3.5, enzyme expressed in Pichia pastoris (pGAPZalphaA). The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
0.856
myo-inositol hexakisphosphate
pH 6.0, 40°C, recombinant His-tagged C-terminal domain
0.858
myo-inositol hexakisphosphate
pH 6.0, 40°C, recombinant His-tagged full-length enzyme
0.91
myo-inositol hexakisphosphate
-
-
0.98
myo-inositol hexakisphosphate
-
-
1
myo-inositol hexakisphosphate
-
immobilized enzyme
1.073
myo-inositol hexakisphosphate
pH 7.0, 65°C
1.125
myo-inositol hexakisphosphate
pH 7.5, 70°C
1.2
myo-inositol hexakisphosphate
-
37°C, pH 3.5, enzyme expressed in Saccharomyces cerevisiae. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
1.4
myo-inositol hexakisphosphate
pH 7.0, 37°C, recombinant mutant D24G
1.45
myo-inositol hexakisphosphate
-
5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60°C
1.5
myo-inositol hexakisphosphate
-
37°C, pH 5.5, enzyme expressed in Schizosaccharomyces pombe. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
1.5
myo-inositol hexakisphosphate
pH 7.0, 37°C, recombinant mutant D24G/K70R/K111E/N121S
1.77
myo-inositol hexakisphosphate
pH 7.0, 37°C, recombinant mutant D24G/K265N
1.8
myo-inositol hexakisphosphate
-
37°C, pH 5.5, enzyme expressed in Pichia pastoris (pGAPZalphaA) or Pichia pastoris (pPICZalphaA). The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
2
myo-inositol hexakisphosphate
-
-
2.1
myo-inositol hexakisphosphate
-
-
2.1
myo-inositol hexakisphosphate
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37°C, pH 5.5, enzyme expressed in Saccharomyces cerevisiae. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
2.19
myo-inositol hexakisphosphate
pH 7.0, 37°C, recombinant wild-type enzyme
2.8
myo-inositol hexakisphosphate
-
-
2.84
myo-inositol hexakisphosphate
-
-
2.84
myo-inositol hexakisphosphate
-
pH 5.5
3
myo-inositol hexakisphosphate
-
pH 2.5, 15°C
114.8
myo-inositol hexakisphosphate
-
potassium salt, pH 5.5, 50°C
0.14
myo-inositol hexakisphosphate disodium salt
pH 5.5, 50°C
0.15
myo-inositol hexakisphosphate disodium salt
pH 5.5, 50°C
0.08
myo-inositol hexakisphosphate sodium salt
pH 5.5, 50°C
0.16
myo-inositol hexakisphosphate sodium salt
pH 5.5, 50°C
0.035
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme a1, pH 5.0, 36°C
0.036
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme a, pH 5.0, 36°C
0.045
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 2.5, 42°C, recombinant mutant C435G
0.045
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme b1, pH 5.0, 36°C
0.046
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme b2, pH 5.0, 36°C
0.048
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant isozyme b, pH 5.0, 36°C
0.056
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 6.0, 37°C, recombinant mutant C264G
0.07
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 5.0, 58°C, recombinant wild-type enzyme
0.13
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
isozyme RO2, pH 4.5, 40°C
0.145
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 5.0, 37°C, recombinant mutant C215S
0.18
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 5.5, 53°C, recombinant mutant C31G
0.19
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant wild-type enzyme, pH 4.5, 37°C
0.191
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase 168PhyA, pH 7.0, 55°C
0.2
myo-inositol-1,2,3,4,5,6-hexakisphosphate
pH 4.0, 60°C
0.24
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase 168PhyA, pH 7.0, 55°C
0.42
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
pH 7.0, 37°C
0.45
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant enzyme mutant R79L, pH 4.5, 37°C
0.5
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
dual domain phytase PhyH, pH 7.0, 35°C
0.52
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
pH 5.5, 50°C
0.55
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant enzyme mutant R79S, pH 4.5, 37°C
0.69
myo-inositol-1,2,3,4,5,6-hexakisphosphate
recombinant enzyme mutant R79G, pH 4.5, 37°C
1.086
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase PhyP, pH 7.0, 37°C
1.28
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase PhyP, pH 7.0, 37°C
1.432
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DII, pH 7.0, 35°C
1.6
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
isozyme RO1, pH 4.5, 40°C
1.98
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant E121F, pH 5.0, 37°C
3.024
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant K41E, pH 5.0, 37°C
8.442
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C
0.74
O-phospho-L-serine
-
pH 5.0, 35°C, phytase LP12
0.8 - 11
O-phospho-L-serine
-
pH 5.0, 35°C, phytase LP2
0.891
O-phospho-L-serine
-
pH 5.0, 35°C, phytase LP11
0.52
p-nitrophenyl phosphate
-
at 62°C, in 50 mM sodium acetate buffer (pH 5)
2.27
p-nitrophenyl phosphate
-
in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37°C
12.4
p-nitrophenyl phosphate
-
37°C, pH 4.5
18.16
p-nitrophenyl phosphate
in 1 M sodium acetate buffer pH 5.5, at 37°C
0.087 - 0.324
phytate
-
pH 2.5, 55°C
0.138
phytate
-
wild-type enzyme
0.14
phytate
pH 5.0, 37°C
0.25 - 0.425
phytate
pH 4.0, 55°C
0.67
phytate
pH 7.5, 37°C
0.747
phytate
-
mutant enzyme E227A
0.914
phytate
-
mutant enzyme D55A
0.934
phytate
-
mutant enzyme Y159H
0.967
phytate
-
mutant enzyme K76E
2.505
phytate
-
mutant enzyme R122E
3.046
phytate
-
mutant enzyme R122K
3.542
phytate
-
mutant enzyme D258A
8.661
phytate
-
mutant enzyme Y159A
11.99
phytate
-
mutant enzyme K76R
0.776
pyridoxal phosphate
-
pH 5.0, 35°C, phytase LP11
0.876
pyridoxal phosphate
-
pH 5.0, 35°C, phytase LP12
0.915
pyridoxal phosphate
-
pH 5.0, 35°C, phytase LP2
additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
KM873028
Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
thermodynamics
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additional information
additional information
kinetics and thermodynamics, overview
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additional information
additional information
substrate specificity and kinetics of recombinant isozymes, overview
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additional information
additional information
substrate specificity and kinetics of recombinant isozymes, overview
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additional information
additional information
substrate specificity and kinetics of recombinant isozymes, overview
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additional information
additional information
substrate specificity and kinetics of recombinant isozymes, overview
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additional information
additional information
substrate specificity and kinetics of recombinant isozymes, overview
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additional information
additional information
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typical Michaelis-Menten kinetics
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additional information
additional information
typical Michaelis-Menten kinetics
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additional information
additional information
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typical Michaelis-Menten kinetics
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additional information
additional information
Lineweaver-Burk kinetics
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additional information
additional information
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Lineweaver-Burk plots
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additional information
additional information
enzyme kinetics, also with other substrates, such as AMP, NADP, pNPP, 1-naphthyl phosphate, glucose phosphates, beta-glycerol phosphate, and diphosphate, overview
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additional information
additional information
kinetics and thermodynamics of recombinant full-length enzyme and C-terminal domain, overview
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additional information
additional information
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kinetics and thermodynamics of recombinant full-length enzyme and C-terminal domain, overview
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additional information
additional information
Line-Weaver Burk plot
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additional information
additional information
Michaelis-Menten kinetics and Lineweaver-Burk plots
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additional information
additional information
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Michealis-Menten kinetics
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additional information
additional information
thermodynamics of the recombinant enzyme
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