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Literature summary for 3.1.3.8 extracted from
- Mining of ruminant microbial phytase (RPHY1) from metagenomic data of Mehsani buffalo breed identification, gene cloning, and characterization (2016), J. Mol. Microbiol. Biotechnol., 26, 252-260 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | strong activation | Prevotella ruminicola |  |
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | the RPHY1 gene mined from rumen proves its promising candidature as a feed supplement enzyme in animal farming | Prevotella ruminicola |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Prevotella ruminicola |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight inhibition | Prevotella ruminicola | |
| K+ | strong inhibition | Prevotella ruminicola | |
| Mg2+ | strong inhibition | Prevotella ruminicola | |
| Mn2+ | slight inhibition | Prevotella ruminicola | |
| Na+ | slight inhibition | Prevotella ruminicola | |
| SDS | - |
Prevotella ruminicola | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Prevotella ruminicola | |
| 0.757 | - |
myo-inositol hexakisphosphate | pH 5.0, 55°C, recombinant His-tagged enzyme | Prevotella ruminicola | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | Prevotella ruminicola | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prevotella ruminicola | A0A125R6G8 | Bacteroides ruminicola, isolated from Mehsani buffalo rumen | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Prevotella ruminicola |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| purified and acetone-precipitated protein is obtained using denaturing and refolding on a nickel affinity chromatography column | Prevotella ruminicola |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1952 | - |
purified recombinant His-tagged enzyme, pH 5.0, 55°C | Prevotella ruminicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Prevotella ruminicola | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 47750, sequence calculation | Prevotella ruminicola |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | cf. EC 3.1.3.26 | Prevotella ruminicola |
| myo-inositol hexakisphosphate hydrolase | - |
Prevotella ruminicola |
| phytase | - |
Prevotella ruminicola |
| RPHY1 | - |
Prevotella ruminicola |
| ruminant microbial phytase | - |
Prevotella ruminicola |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
recombinant His-tagged enzyme | Prevotella ruminicola |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | activity range, profile overview | Prevotella ruminicola |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
15 | purified recombinant His-tagged enzyme, over 50% activity remaining | Prevotella ruminicola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant His-tagged enzyme | Prevotella ruminicola |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 10 | activity range, profile overview | Prevotella ruminicola |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 7 | purified recombinant His-tagged enzyme, over 50% activity remaining | Prevotella ruminicola |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Prevotella ruminicola | sequence calculation | - |
7.15 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme structure possesses a phosphate-binding pocket with residues R54, H55, R58, I61, R146, W272, H315, E316, and V317 involved in phosphate ligand binding | Prevotella ruminicola |
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