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Literature summary for 3.1.3.8 extracted from

  • Rodriguez, E.; Mullaney, E.J.; Lei, X.G.
    Expression of the Aspergillus fumigatus phytase gene in Pichia pastoris and characterization of the recombinant enzyme (2000), Biochem. Biophys. Res. Commun., 268, 373-378.
    View publication on PubMed
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.38
-
 4-nitrophenyl phosphate
-
 Aspergillus fumigatus
1.38
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
2.84
-
 myo-inositol hexakisphosphate
-
 Aspergillus fumigatus
2.84
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 x * 49000, SDS-PAGE after deglycosylation Aspergillus fumigatus
76000
-
 1 * 76000, SDS-PAGE Aspergillus fumigatus
76000
-
 x * 76000, SDS-PAGE before deglycosylation Aspergillus fumigatus
92000
-
 nondenaturing PAGE Aspergillus fumigatus
92000
-
 non-denaturing PAGE Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 36% glycosylation Aspergillus fumigatus
side-chain modification 36% glycosylation Aspergillus fumigatus

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Aspergillus fumigatus 4-nitrophenol + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus ? + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE before deglycosylation Aspergillus fumigatus
? x * 49000, SDS-PAGE after deglycosylation Aspergillus fumigatus
monomer 1 * 76000, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 80 about 45% of maximal activity at 37°C and at 80°C Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 better thermostability at 65°C and at 90°C when sodium acetate is used as the buffer, pH 5.5, than that of sodium citrate at either low, 10 mM or high, 0.2 M, concentration Aspergillus fumigatus
37
-
 20 min, less than 5% of activity of enzyme in 0.01 M sodium citrate buffer, about 5% loss of activity in 0.2 M sodium citrate buffer, about 10% loss of activity in 0.2 M sodium acetate buffer, about 15% loss of activity in 0.01 sodium acetate bugffer Aspergillus fumigatus
65
-
 20 min, about 35% loss of activity in 0.01 M sodium acetate buffer, about 40% loss of activity in 0.2 M acetate buffer, about 45% loss of activity in 0.01 M sodium citrate buffer, about 65% loss of activity in 0.2 M sodium citrate buffer Aspergillus fumigatus
90
-
 20 min, about 35% loss of activity in 0.01 M sodium acetate buffer, about 40% loss of activity in 0.2 M acetate buffer, about 45% loss of activity in 0.01 M sodium citrate buffer, about 65% loss of activity in 0.2 M sodium citrate buffer Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
99
-
 4-nitrophenyl phosphate
-
 Aspergillus fumigatus
114
-
 myo-inositol hexakisphosphate
-
 Aspergillus fumigatus
357500
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
358000
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
411700
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus
412000
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus fumigatus
6
-
 and a second lower optimum at pH 4.0, reaction with phytate Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7 pH 3.5: about 35% of maximal activity, pH 7.0: about 70% of maximal activity Aspergillus fumigatus
3.5 7 pH 3.5: about 35% of maximal activity, pH 7.0: about 70% of maximal activity, reaction with phytate Aspergillus fumigatus