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Literature summary for 3.1.3.8 extracted from

  • Sajidan, A.; Farouk, A.; Greiner, R.; Jungblut, P.; Muller, E.C.; Borriss, R.
    Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp. ASR1 (2004), Appl. Microbiol. Biotechnol., 65, 110-117.
    View publication on PubMed
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Klebsiella sp.

Organism

Organism UniProt Comment Textmining
Klebsiella sp.
-
 ASR1
-
Klebsiella sp. ASR1
-
 ASR1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
169
-
-
 Klebsiella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O 2.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 1-naphthol + phosphate
-
 ?
2-glycerophosphate + H2O 19.8% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. glycerate + phosphate
-
 ?
2-naphthyl phosphate + H2O 4.9% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 2-naphthol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 23.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. 4-nitrophenol + phosphate
-
 ?
ADP + H2O 7.5% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ?
-
 ?
ADP + H2O 7.5% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 ?
-
 ?
AMP + H2O 9.6% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. adenosine + phosphate
-
 ?
ATP + H2O 5.6% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ?
-
 ?
D-fructose 6-phosphate + H2O 8.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. D-fructose + phosphate
-
 ?
D-fructose 6-phosphate + H2O 8.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 D-fructose + phosphate
-
 ?
fructose 1,6-diphosphate + H2O 12.3% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ?
-
 ?
fructose 1,6-diphosphate + H2O 12.3% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 ?
-
 ?
Glucose 6-phosphate + H2O 2.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. Glucose + phosphate
-
 ?
Glucose 6-phosphate + H2O 2.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. ASR1 Glucose + phosphate
-
 ?
additional information no activity with GTP and diphosphate Klebsiella sp. ?
-
 ?
additional information no activity with GTP and diphosphate Klebsiella sp. ASR1 ?
-
 ?
myo-inositol hexakisphosphate + H2O step-wise dephosphorylation occurs via 1. D-myo-inositol 1,2,4,5,6-pentakisphosphate, 2. D-myo-inositol 1,2,5,6-tetrakisphosphate or D-myo-inositol 2,4,5,6-tetrakisphosphate, 3. D-myo-inositol 1,2,6-trisphosphate, D-myo-inositol 1,2,3-trisphosphate or D-myo-inositol 1,4,5-trisphosphate, 4. myo-inositol 1,2-bisphosphate or myo-inositol 2,5-bisphosphate, myo-inositol 4,5-bisphosphate or myo-inositol 2,4-bisphosphate. Myo-inositol 2-monophosphate is the final product Klebsiella sp. D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
NADP+ + H2O 58.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme Klebsiella sp. NAD+ + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Klebsiella sp.

Synonyms

Synonyms Comment Organism
PhyK
-
 Klebsiella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Klebsiella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 15 min, stable below Klebsiella sp.
65
-
 15 min, complete inactivation Klebsiella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Klebsiella sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 7 enzyme is virtually inactive below pH 4.0 and above pH 7.0 Klebsiella sp.