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Literature summary for 3.1.3.8 extracted from
- Characterization of recombinant fungal phytase (phyA) expressed in tobacco leaves (1999), Biochem. Biophys. Res. Commun., 264, 201-206.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Nicotiana tabacum leaves | Aspergillus ficuum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| myo-inositol hexasulfate | - |
Aspergillus ficuum |  |
| Phenylglyoxal | - |
Aspergillus ficuum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
myo-inositol hexakisphosphate | native fungal enzyme | Aspergillus ficuum | |
| 0.065 | - |
myo-inositol hexakisphosphate | recombinant enzyme | Aspergillus ficuum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus ficuum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein | Aspergillus ficuum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Aspergillus ficuum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus ficuum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
recombinant and native enzyme | Aspergillus ficuum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 348 | - |
myo-inositol hexakisphosphate | native fungal enzyme | Aspergillus ficuum | |
| 360 | - |
myo-inositol hexakisphosphate | recombinant enzyme | Aspergillus ficuum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
recombinant enzyme | Aspergillus ficuum |
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Release 2023.1 (January 2023)
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