Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Q258N | substitution introduced to enhance the glycosylation activity upon expression in Pichia pastoris | Escherichia coli |
Q258N/Q349N | substitutions introduced to enhance the glycosylation activity upon expression in Pichia pastoris. Mutant displays 40% enhancement in thermostability compared to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
myo-inositol hexakisphosphate | mutant Q258N/Q349N, pH 4.5, 37°C | Escherichia coli | |
0.48 | - |
myo-inositol hexakisphosphate | wild-type, pH 4.5, 37°C | Escherichia coli | |
0.52 | - |
myo-inositol hexakisphosphate | mutant Q258N, pH 4.5, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE, wild-type | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | after treatment with EndoH, protein gives one band of 50 kDa in SDS-PAGE | Escherichia coli |
Purification (Comment) | Organism |
---|---|
recombinant protein | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3137 | - |
mutant Q258N/Q349N, pH 4.5, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Escherichia coli | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE, wild-type | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AppA | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
67.5 | - |
melting temperature, wild-type | Escherichia coli |
72.2 | - |
melting temperature, mutant Q258N/Q349N | Escherichia coli |
85 | - |
10 min, 60% residual activity for mutant Q258N/Q349N, 5 min, 30% residual activiy for wild-type | Escherichia coli |
100 | - |
10 min, 40% residual activity for mutant Q258N/Q349N | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2660 | - |
myo-inositol hexakisphosphate | mutant Q258N, pH 4.5, 37°C | Escherichia coli | |
3058 | - |
myo-inositol hexakisphosphate | mutant Q258N/Q349N, pH 4.5, 37°C | Escherichia coli | |
3397 | - |
myo-inositol hexakisphosphate | wild-type, pH 4.5, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
both wild-typ and mutants Q258N and Q258N/Q349N | Escherichia coli |