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Literature summary for 3.1.3.8 extracted from

  • Sapna, C.M.; Singh, B.
    Purification and characterization of a protease-resistant phytase of Aspergillus oryzae SBS50 whose properties make it exceptionally useful as a feed supplement (2017), Int. J. Biol. Macromol., 103, 458-466 .
    View publication on PubMed

Application

Application Comment Organism
agriculture the phytase has the potential to be useful as an animal feed supplement. Among all the feed samples, mustard oil cake is dephytinized more efficiently than other feed samples Aspergillus oryzae

General Stability

General Stability Organism
substrate calcium phytate stablizes the enzyme, while sucrose, glycerol, and starch do not Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione inhibits 28% at 1 mM and 52% at 5 mM Aspergillus oryzae
2-mercaptoethanol inhibits 20% at 1 mM and 60% at 5 mM Aspergillus oryzae
ethylmaleimide inhibits about 10% at 1-5 mM Aspergillus oryzae
Fe2+ inhibits 97% at 1 mM and 99% at 5 mM Aspergillus oryzae
Fe3+ inhibits 30% at 1 mM and 83% at 5 mM Aspergillus oryzae
iodoacetamide inhibits 32% at 1 mM and 36% at 5 mM Aspergillus oryzae
isoamyl alcohol inhibits 31% at 2% v/v and 38% at 5% v/v Aspergillus oryzae
KI inhibits 31% at 1 M and 44% at 2 M Aspergillus oryzae
Mn2+ activates 40% at 1 mM and inhibits 65% at 5 mM Aspergillus oryzae
additional information the phytase of Aspergillus oryzae shows protease resistance. Poor effect by urea at 1-2 M and by EDTA at 1-5 mM Aspergillus oryzae
SDS inhibits 42% at 0.5% Aspergillus oryzae
Sodium molybdate inhibits 54% at 1 mM and 80% at 5 mM Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus oryzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
acetone activates by 35% at 5% v/v Aspergillus oryzae
butanol activates by 39% at 5% v/v Aspergillus oryzae
Ca2+ activates Aspergillus oryzae
Co2+ activates Aspergillus oryzae
ethanol activates by 54% at 5% v/v Aspergillus oryzae
methanol activates by 54% at 5% v/v Aspergillus oryzae
Mg2+ activates Aspergillus oryzae
Mn2+ activates 40% at 1 mM and inhibits 65% at 5 mM Aspergillus oryzae
NaCl activates at 1 mM by 58%, inhibits at 5 mM by 18% Aspergillus oryzae
Triton X-100 activates at 0.5% Aspergillus oryzae
Tween 60 activates at 0.1-0.5% Aspergillus oryzae
Tween 80 activates at 0.1-0.5% Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65500
-
gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Aspergillus oryzae
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Aspergillus oryzae SBS50
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q1KNE3 isolated from a soil sample collected from Rohtak, Haryana (India)
-
Aspergillus oryzae SBS50 Q1KNE3 isolated from a soil sample collected from Rohtak, Haryana (India)
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 10.68fold by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration to homogeneity Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.63
-
purified enzyme, pH 5.0, 50°C, substrate calcium phytate Aspergillus oryzae

Storage Stability

Storage Stability Organism
4°C, pH 5.0, the purified enzyme retains 80% after 6 months Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows a broad substrate specificity with highest activity for calcium phytate, the enzyme is also active with ADP, ATP, glycerol beta-phosphate, glucose 6-phosphate, and 4-nitrophenyl phosphate. Among all the feed samples, mustard oil cake is dephytinized more efficiently than otherfeed samples, e.g. cotton oil cake Aspergillus oryzae ?
-
?
additional information the enzyme shows a broad substrate specificity with highest activity for calcium phytate, the enzyme is also active with ADP, ATP, glycerol beta-phosphate, glucose 6-phosphate, and 4-nitrophenyl phosphate. Among all the feed samples, mustard oil cake is dephytinized more efficiently than otherfeed samples, e.g. cotton oil cake Aspergillus oryzae SBS50 ?
-
?
myo-inositol hexakisphosphate + H2O
-
Aspergillus oryzae 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O best substrate is calcium phytate, sodium phytate results in 60% activity compared to calcium phytate Aspergillus oryzae 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Aspergillus oryzae SBS50 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O best substrate is calcium phytate, sodium phytate results in 60% activity compared to calcium phytate Aspergillus oryzae SBS50 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 80000, native PAGE Aspergillus oryzae
monomer 1 * 80000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
PhyA
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range, profile overview Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified extracellular enzyme, half-life is 300 min Aspergillus oryzae
80
-
purified extracellular enzyme, about 35 min, inactivation Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
3 8 activity range, profile overview Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5 purified extracellular enzyme, 120 min, 50°C, loss of 20% activity Aspergillus oryzae
7
-
purified extracellular enzyme, 120 min, 50°C, loss of 60% activity Aspergillus oryzae