Application | Comment | Organism |
---|---|---|
agriculture | the phytase has the potential to be useful as an animal feed supplement. Among all the feed samples, mustard oil cake is dephytinized more efficiently than other feed samples | Aspergillus oryzae |
General Stability | Organism |
---|---|
substrate calcium phytate stablizes the enzyme, while sucrose, glycerol, and starch do not | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Butanedione | inhibits 28% at 1 mM and 52% at 5 mM | Aspergillus oryzae | |
2-mercaptoethanol | inhibits 20% at 1 mM and 60% at 5 mM | Aspergillus oryzae | |
ethylmaleimide | inhibits about 10% at 1-5 mM | Aspergillus oryzae | |
Fe2+ | inhibits 97% at 1 mM and 99% at 5 mM | Aspergillus oryzae | |
Fe3+ | inhibits 30% at 1 mM and 83% at 5 mM | Aspergillus oryzae | |
iodoacetamide | inhibits 32% at 1 mM and 36% at 5 mM | Aspergillus oryzae | |
isoamyl alcohol | inhibits 31% at 2% v/v and 38% at 5% v/v | Aspergillus oryzae | |
KI | inhibits 31% at 1 M and 44% at 2 M | Aspergillus oryzae | |
Mn2+ | activates 40% at 1 mM and inhibits 65% at 5 mM | Aspergillus oryzae | |
additional information | the phytase of Aspergillus oryzae shows protease resistance. Poor effect by urea at 1-2 M and by EDTA at 1-5 mM | Aspergillus oryzae | |
SDS | inhibits 42% at 0.5% | Aspergillus oryzae | |
Sodium molybdate | inhibits 54% at 1 mM and 80% at 5 mM | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus oryzae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
acetone | activates by 35% at 5% v/v | Aspergillus oryzae | |
butanol | activates by 39% at 5% v/v | Aspergillus oryzae | |
Ca2+ | activates | Aspergillus oryzae | |
Co2+ | activates | Aspergillus oryzae | |
ethanol | activates by 54% at 5% v/v | Aspergillus oryzae | |
methanol | activates by 54% at 5% v/v | Aspergillus oryzae | |
Mg2+ | activates | Aspergillus oryzae | |
Mn2+ | activates 40% at 1 mM and inhibits 65% at 5 mM | Aspergillus oryzae | |
NaCl | activates at 1 mM by 58%, inhibits at 5 mM by 18% | Aspergillus oryzae | |
Triton X-100 | activates at 0.5% | Aspergillus oryzae | |
Tween 60 | activates at 0.1-0.5% | Aspergillus oryzae | |
Tween 80 | activates at 0.1-0.5% | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65500 | - |
gel filtration | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Aspergillus oryzae | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | Aspergillus oryzae SBS50 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q1KNE3 | isolated from a soil sample collected from Rohtak, Haryana (India) | - |
Aspergillus oryzae SBS50 | Q1KNE3 | isolated from a soil sample collected from Rohtak, Haryana (India) | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 10.68fold by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration to homogeneity | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.63 | - |
purified enzyme, pH 5.0, 50°C, substrate calcium phytate | Aspergillus oryzae |
Storage Stability | Organism |
---|---|
4°C, pH 5.0, the purified enzyme retains 80% after 6 months | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows a broad substrate specificity with highest activity for calcium phytate, the enzyme is also active with ADP, ATP, glycerol beta-phosphate, glucose 6-phosphate, and 4-nitrophenyl phosphate. Among all the feed samples, mustard oil cake is dephytinized more efficiently than otherfeed samples, e.g. cotton oil cake | Aspergillus oryzae | ? | - |
? | |
additional information | the enzyme shows a broad substrate specificity with highest activity for calcium phytate, the enzyme is also active with ADP, ATP, glycerol beta-phosphate, glucose 6-phosphate, and 4-nitrophenyl phosphate. Among all the feed samples, mustard oil cake is dephytinized more efficiently than otherfeed samples, e.g. cotton oil cake | Aspergillus oryzae SBS50 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus oryzae | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | best substrate is calcium phytate, sodium phytate results in 60% activity compared to calcium phytate | Aspergillus oryzae | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus oryzae SBS50 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | best substrate is calcium phytate, sodium phytate results in 60% activity compared to calcium phytate | Aspergillus oryzae SBS50 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 80000, native PAGE | Aspergillus oryzae |
monomer | 1 * 80000, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
PhyA | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | activity range, profile overview | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified extracellular enzyme, half-life is 300 min | Aspergillus oryzae |
80 | - |
purified extracellular enzyme, about 35 min, inactivation | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | activity range, profile overview | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | purified extracellular enzyme, 120 min, 50°C, loss of 20% activity | Aspergillus oryzae |
7 | - |
purified extracellular enzyme, 120 min, 50°C, loss of 60% activity | Aspergillus oryzae |