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Literature summary for 3.1.3.8 extracted from

  • Lung, S.C.; Leung, A.; Kuang, R.; Wang, Y.; Leung, P.; Lim, B.L.
    Phytase activity in tobacco (Nicotiana tabacum) root exudates is exhibited by a purple acid phosphatase (2008), Phytochemistry, 69, 365-373.
    View publication on PubMed
Search for more literature for 3.1.3.8

Inhibitors

Inhibitors Comment Organism Structure
molybdate 95% inhibition at 0.0625 mM Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0139
-
 myo-inositol hexakisphosphate in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C Nicotiana tabacum
0.0147
-
 myo-inositol hexakisphosphate in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 gel filtration Nicotiana tabacum
64000
-
 SDS-PAGE Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono S column chromatography and Superose 12 HR 10/30 gel filtration Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.744
-
 root exsudate, at 37°C Nicotiana tabacum
361.7
-
 after 486fold purification, at 37°C Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phosphoglycerate + H2O 168% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum glycerate + phosphate
-
 ?
ADP + H2O 206% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum AMP + phosphate
-
 ?
AMP + H2O 30% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum adenosine + phosphate
-
 ?
ATP + H2O 365% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum ADP + phosphate
-
 ?
CTP + H2O 352% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum CDP + phosphate
-
 ?
D-fructose 6-phosphate + H2O 75% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum D-fructose + phosphate
-
 ?
D-glucose 1-phosphate + H2O 5% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum D-glucose + phosphate
-
 ?
D-glucose 3-phosphate + H2O 75% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 57% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum D-glucose + phosphate
-
 ?
D-ribose 5-phosphate + H2O 75% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum D-ribose + phosphate
-
 ?
dATP + H2O 338% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum dADP + phosphate
-
 ?
dCTP + H2O 367% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum dCDP + phosphate
-
 ?
dGTP + H2O 189% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum dGDP + phosphate
-
 ?
dTTP + H2O 389% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum dTDP + phosphate
-
 ?
GTP + H2O 271% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum GDP + phosphate
-
 ?
L-tyrosine phosphate + H2O 142% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum L-tyrosine + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O 100% relative activity Nicotiana tabacum 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
p-nitrophenyl phosphate + H2O 311% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum p-nitrophenol + phosphate
-
 ?
phenyl phosphate + H2O 188% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum phenol + phosphate
-
 ?
phosphoenolpyruvate + H2O 328% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum pyruvate + phosphate
-
 ?
UTP + H2O 350% relative activity compared to myo-inositol hexakisphosphate Nicotiana tabacum UDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 56000, gel filtration Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Nicotiana tabacum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5 6
-
 Nicotiana tabacum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 60 the enzyme is stable at or below 55°C exhibiting at least 80% of residual activity, and is completely denatured at 60°C or above Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
908
-
 myo-inositol hexakisphosphate in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C Nicotiana tabacum
2056
-
 myo-inositol hexakisphosphate in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C Nicotiana tabacum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 complete abolishment of enzyme activity at or above pH 6.5 Nicotiana tabacum