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Literature summary for 3.1.3.8 extracted from

  • Huang, H.; Luo, H.; Wang, Y.; Fu, D.; Shao, N.; Wang, G.; Yang, P.; Yao, B.
    A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under low pH and strong pepsin conditions (2008), Appl. Microbiol. Biotechnol., 80, 417-426.
    View publication on PubMed

Application

Application Comment Organism
nutrition use of Yersinia rohdei phytase as an attractive additive to animal feed. Compared with the major commercial phytases from Aspergillus niger, Escherichia coli, and a potential commercial phytase from Yersinia intermedia, the Yersinia rohdei phytase is more resistant to pepsin, retains more activity under gastric conditions, and releases more inorganic phosphorus from soybean meal under simulated gastric conditions Yersinia rohdei

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Yersinia rohdei
expression in Pichia pastoris Yersinia pestis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.196
-
myo-inositol hexakisphosphate pH 4.5, 37°C Yersinia pestis
0.206
-
myo-inositol hexakisphosphate pH 4.5, 37°C Yersinia rohdei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45200
-
x * 46000, SDS-PAGE, x * 45200, calculated Yersinia pestis
46000
-
x * 46000, SDS-PAGE, x * 45200, calculated Yersinia pestis
46000
-
x * 46000, SDS-PAGE, x * 46100, calculated Yersinia rohdei
46100
-
x * 46000, SDS-PAGE, x * 46100, calculated Yersinia rohdei

Organism

Organism UniProt Comment Textmining
Yersinia pestis Q7CIZ7
-
-
Yersinia rohdei B4X9S4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Yersinia rohdei
recombinant protein Yersinia pestis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2344
-
pH 4.5, 37°C Yersinia pestis
2456
-
pH 4.5, 37°C Yersinia rohdei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
Yersinia rohdei 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Yersinia pestis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE, x * 45200, calculated Yersinia pestis
? x * 46000, SDS-PAGE, x * 46100, calculated Yersinia rohdei

Synonyms

Synonyms Comment Organism
AppA
-
Yersinia pestis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Yersinia rohdei
55
-
-
Yersinia pestis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
2 min, 35% residual activity Yersinia pestis
80
-
2 min, 50% residual activity Yersinia rohdei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2283
-
myo-inositol hexakisphosphate pH 4.5, 37°C Yersinia pestis
2531
-
myo-inositol hexakisphosphate pH 4.5, 37°C Yersinia rohdei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Yersinia rohdei
4.5
-
-
Yersinia pestis

pH Range

pH Minimum pH Maximum Comment Organism
2 5.5
-
Yersinia pestis
2 5.5 more than 80% of maximum activity Yersinia rohdei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.6
-
myo-inositol hexakisphosphate pH 4.5, 37°C Yersinia pestis
12.3
-
myo-inositol hexakisphosphate pH 4.5, 37°C Yersinia rohdei