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Literature summary for 3.1.3.8 extracted from

  • Rocky-Salimi, K.; Hashemi, M.; Safari, M.; Mousivand, M.
    A novel phytase characterized by thermostability and high pH tolerance from rice phyllosphere isolated Bacillus subtilis B.S.46 (2016), J. Adv. Res., 7, 381-390 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information the enzyme is useful as animal feed additive, in dephytinization of food ingredients, and bioremediation of phosphorous pollution in the environment Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 47% activation at 1 mM, 54% inhibition at 5 mM, 90% at 10 mM Bacillus subtilis
Cu2+ 8% inhibition at 1 mM, 92% inhibition at 5 mM; complete inhibition Bacillus subtilis
Fe2+ 30% inhibition at 1 mM, complete inhibition at 2 mM; complete inhibition Bacillus subtilis
Hg2+ complete inhibition; complete inhibition at 1-10 mM Bacillus subtilis
K+ about 10% inhibition at 2-10 mM Bacillus subtilis
Mg2+ 6% inhibition at 1 mM, 90% inhibition at 5 mM; complete inhibition Bacillus subtilis
Mn2+ 92% inhibition at 1 mM, complete inhibition at 5 mM; complete inhibition Bacillus subtilis
Zn2+ 63% inhibition at 1 mM, 88% inhibition at 5 mM; complete inhibition Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus subtilis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 47% activation at 1 mM, 54% inhibition at 5 mM, 90% at 10 mM Bacillus subtilis
Li+ activates at 1-10 mM, 20% at 2 mM Bacillus subtilis
additional information poor inhibition by Na+ at 1-10 mM Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Bacillus subtilis
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Bacillus subtilis B.S.46
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
isolated from Oryza sativa phyllosphere
-
Bacillus subtilis B.S.46
-
isolated from Oryza sativa phyllosphere
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by ammonium sulfate fractionation and dialysis Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.45
-
partially purified enzyme, pH 7.0-7.5, 50-55°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
Bacillus subtilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O highest activity at 2.05 mM substrate Bacillus subtilis 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Bacillus subtilis B.S.46 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O highest activity at 2.05 mM substrate Bacillus subtilis B.S.46 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
myo-inositol 1,2,3,4,5,6-hexakisphosphate phosphohydrolase
-
Bacillus subtilis
phytase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
56.5
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
47 68 60-73% of maximal activity within this range Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
partially purified enzyme, retains 83% and 60% of its initial activity after 90 and 120 min, respectively Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
6.3 8 60-73% of maximal activity within this range Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5 partially purified enzyme, 30 min, complete inactivation Bacillus subtilis
6 7 partially purified enzyme, 30 min, completely stable at, 85% activity remains after 24 h Bacillus subtilis
8 10 partially purified enzyme, 30 min, 1.16fold activation, completely stable for 24 h Bacillus subtilis