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3.1.3.4: phosphatidate phosphatase

This is an abbreviated version!
For detailed information about phosphatidate phosphatase, go to the full flat file.

Word Map on EC 3.1.3.4

Reaction

a 1,2-diacylglycerol 3-phosphate
+
H2O
=
a 1,2-diacyl-sn-glycerol
 +
phosphate

Synonyms

3-sn-phosphatidate phosphohydrolase, acid phosphatidyl phosphatase, APP1, DGPP phosphatase, diacylglycerol diphosphate phosphatase, diacylglycerol pyrophosphate phosphatase, DPP1, Dpp1p, DPPL1, DPPL2, Dri-42, Dri42, ecto-PAPase, ecto-phosphatidic acid phosphohydrolase, EtLPP1, EtLPP2, EtLPP3, Germ cell guidance factor, Ippalpha, Ippbeta, Laza, Lazaro phosphatidic acid phosphatase, lipid phosphate phosphatase, lipid phosphate phosphatase 1, lipid phosphate phosphatase 3, lipid phosphate phosphatase-1, lipid phosphate phosphatase-2, lipid phosphate phosphatase-3, lipid phosphate phosphatase-related protein, lipid phosphate phosphatase-related protein type 1, lipid phosphate phosphatase-related protein type 2, lipid phosphate phosphatase-related protein type 3, lipid phosphate phosphatase-related protein type 4, lipid phosphate phosphohydrolase, lipidphosphatase-related protein 1, lipin, lipin 1, lipin 1beta, lipin 2, lipin 3, lipin Pah1p/Smp2p, lipin-1, lipin-1alpha, lipin-1beta, lipin-1gamma, lipin-2, lipin-3, Lipin1, lipin1alpha, lipin1beta, LLP1, LLP2, LPIN1, LPP, LPP-1, LPP1, LPP1-encoded lipid phosphatase, Lpp1p, LPP2, LPP3, LPPR2, LPR, LPR-1, LPR-2, LPR-3, LPR-4, LPR1, Mg2+-dependent PA1 phosphatase, Mg2+-dependent phosphatidate phosphatase, Mg2+-dependent phosphatidic acid phosphatase, More, PA phosphatase, PA-P, PA-PSP, PA2 phosphatase, PAH, pah1, Pah1p, Pah1p/Smp2p, PAH2, PAP, PAP-1, PAP-2, PAP-2b, PAP1, PAP2, PAP2-like protein, PAP2a, PAP2d, PAP2L2, PAP3, PAPase, phosphatidate phosphatase, phosphatidate phosphatase type-1, phosphatidate phosphatase-1, phosphatidate phosphohydrolase, phosphatidic acid phosphatase, phosphatidic acid phosphatase 2a, phosphatidic acid phosphatase type 2, phosphatidic acid phosphatase-1, phosphatidic acid phosphohydrolase, phosphatidic acid phosphohydrolase 1, phosphatidic acid phosphohydrolase-1, plasticity related gene 1, plasticity related gene 3, plasticity related gene 4, presqualene diphosphate phosphatase, PRG-1, PRG-3, PRG3, RHA1_ro00075, SCO1102, SCO1753, SMP2, type 2b phosphatidic acid phosphatase, wun, wun2, WunD, Wunen, Wunen protein, wunen-2, Wunen2

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.4 phosphatidate phosphatase

Cloned

Cloned on EC 3.1.3.4 - phosphatidate phosphatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of two Mg2+-independent isoenzymes, PAP-2a and PAP-2b
-
co-overexpression of lipin with murine LPP-1 in Escherichia coli strain BL21(DE3) as His-tagged enzymes
-
coding sequence of lipin 1b subcloned downstream of the GST tag in the pEBG expression vector, transfected into 293T cells. Adenoviral constructs for HA-tagged lipin 1b
-
expressed as untagged proteins in HUVEC cells or in HEK-293 cells
-
expressed either as untagged proteins or as Flag- or EGFP-tagged enzymes in HUVEC cells or in HEK-293 cells
-
expressed in Escherichia coli BL21-(DE3) cells
-
expressed in HEK293 cells as a green fluorescent fusion protein
-
expressed in P-388D1 cells
-
expression analysis of lipin-1 and lipin-2, recombinant expression of lipin-2 in Hep-G2 cells using an adenoviral vector
-
expression in Escherichia coli
expression in HEK-293 cell
expression of hemagglutinin epitope-tagged phosphatidic acid phosphohydrolase PAP-2a, PAP-2b and PAP-2c in HEK293 cells. Expression of phosphatidic acid phosphohydrolase PAP-2a, PAP-2b and PAP-2c in baculovirus-infected Sf9 insect cells. Expression of PAP-2a but not PAP-2b or PAP-2c results in high levels of cell surface PAP activity in intact insect cells
-
expression of His6-tagged EtLPP1 in HEK293 cells produces immunoreactive proteins with variable molecular sizes, suggesting the presence of multiple forms of enzyme
expression of isozyme DPPL1 in Spodoptera frugiperda Sf9 cells
expression of isozyme DPPL2 in Spodoptera frugiperda Sf9 cells
expression of lipin-1 in HeLa M cells
expression of lipin-1, lipin-2, and lipin-3 in HEK-293 cells
-
expression of lipin-2 in HeLa M cells
-
expression of lipins 1-3 in HEK-293 cells
-
expression of LPP1 in Drosophila melanogaster cells S2
-
expression of Myc-tagged and/or FLAG-tagged LPP1, LLP2, and LLP3 in HEK-293 cells, overview
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expression of PAP2a in Saos-2 cells, promoter determination and anaylsis, expression in HEK-293T cells, expression regulation anaylsis, overview
-
expression of presqualene diphosphate phosphatase in COS-7 cells, co-expression with CTP:phosphocholine cytidylyltransferase-alpha
-
expression of the gene encoding the enzyme under control of chicken beta-actin promoter in mice embryos
-
expression of wild-type and mutant enzymes in rat2 fibroblasts
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full-length cDNAs for human lipin-1 transferred to the pCMV/V5-DEST vector. Plasmid DNA transformed and amplified using DH5alpha bacteria, and transfection of L6 myocytes
-
full-length coding sequence of PAH1 and PAH2 cloned into the pDO105 vector at NotI/MluI sites for PAH1 and NotI/PstI sites for PAH2. Vector constructs introduced into a Saccharomyces cerevisiae DELTAdpp1DELTAlpp1DELTApah1 mutant. Transgenic pah1pah2 plants that harbor either 35S::PAH1-GFP or 35S::PAH2-GFP transgenes
-
full-length lipin1beta cDNA amplified and cloned into the pcDNA3.1-V5/His-TOPO expression vector and pSG5-FLAG-tagged expression vector. Peroxisome proliferator-activated receptor gamma2 and lipin1 overexpressed in NIH3T3 cells
-
full-length of the cDNA of lipin1
full-length triple HA-tagged lipin 2 cloned into pCDNA3 expression construct, subcloned into the Ad-track cytomegalovirus vector, and recombined into the Ad-EASY system. Full-length cDNA for lipin 3 from pSPORT-lipin 3 expression vector
-
functional overexpression of GFP-tagged LPPs in rat2 fibroblasts, the transformed cells show reduced proliferation, overview
-
gene DPP1, functional overexpression of HA-tagged enzyme in the DPP1-deficient mutant Saccharomyces cerevisiae strain DTY1 in vacuole membranes, the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase
-
gene laza, genetic structure and organiszation, expression analysis and pathway regulation, overview
-
gene LPIN2 is located on chromosome 18p11
-
gene Lpp1, stable overexpression of wild-type and mutant LPP1 enzymes in Rat2 fibroblasts using a retroviral vector and puromycin selection
-
gene PAH1, expression in Escherichia coli
-
gene PAH1, overexpression of His-tagged wild-type and mutant isozyme PAH1 in Escherichia coli strain BL21(DE3), co-expression with human lipin, subcloning in Escherichia coli strain DH5alpha
-
genes Lpin1, Lpin2, and Lpin3, expression analysis reveals distinct gene regulation in the hepatocytes
genes VuPAPalpha and VuPAPbeta, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of VuPAPalpha in Escherichia coli
-
genomic library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His6-tagged PAP2L2 in Escherichia coli
HA-tagged forms of wild-type and mutant lipin proteins overexpressed in HEK-293 cells. Expression of HA-lipin in 3T3-L1 adipocytes by adenoviral mediated gene transfer
HEK293 cells or 3T3-L1 adipocytes transfected with wild-type or mutant lipin-1alpha lacking the serine-rich domain
-
isozyme LPPbeta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree
isozyme LPPepsilon1, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation
isozyme LPPepsilon2, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic ARabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation
isozyme LPPgamma, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of isozyme LPPgamma in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation
isozymes LPPdelta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree
lipin-1 adenovirus overexpression
-
lpin-1 coding sequences (with introns) and 3' UTR sequences amplified and recombined into pDONR P1P3. Final product pAG-107 reacted with pCR319 (contains the unc-119(+) gene for transformation rescue of unc-119(ed3) mutants) and pAG-108 (including the lpin-1 promoter) to produce pAG-126
-
LPIN1 reporter plasmid transiently transfected into Hep-G2 cells
-
LPP1 and LPR1 expressed in Sf9 cells using a baculovirus vector. Recombinant LPR1 and mutants incorporating a C-terminal EGFP tag expressed in HeLa and COS7 cells. Overexpression of EGFP-LPR1 in SK-OV-3 cells
LPP1 wild-type or mutants inserted into vector pcDNA3.1 with a C-terminal Myc epitope tag or FLAG epitope tag. HEK-293 cells transfected with plasmid construct encoding catalytically deficient Myc-tagged R127K LPP1 and/or FLAG-tagged H223L LPP1 and/or FLAG-tagged and/or Myc-tagged wild-type
-
LPP1, LPP2 and LPP3
-
LPP2 and LPP3 inserted into vector pcDNA3.1 with a C-terminal Myc epitope tag or FLAG epitope tag and transiently transfected into HEK-293 cells
-
NIH3T3 cells stably expressing LPP1-CFP or LPP3-CFP. MDCK cells stably expressing LPP1-GFP and LPP3-GFP. COS-7 and PC-12 cells transiently expressing LPP1
-
overexpression in HEK-293 cells
-
overexpression in Sf-9 insect cells
-
overexpression in Spodoptera frugiperda Sf9 cells using the baculovirus expression system, and in Saccharomyces cerevisiae using multicopy plasmids
-
overexpression of isozyme LPP1 in Rat2 fibroblasts
-
overexpression of LPP-3 in HEK293 cells and in Xenopus laevis dorsal blastomeres of embryos causing a mild ventralizing effect
overexpression of LPP3 and LPP2 in HEK-293 cells, LPP2 and LPP3 are constitutively co-localized with sphingosine kinase 1, SK1, in cytoplasmic vesicles in HEK-293 cells, LPP2, not LPP3, prevents SK1 from being recruited to the perinuclear space upon induction of phospholipase D1
-
overexpression of LPPs in HEK293 cells, expression of LPP3 in Drosophila melanogaster cells S2
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rat2 fibroblasts infected with adenoviral vectors containing LPP1-myc or LPP1(R217K)-myc. Rat2 fibroblasts stably overexpressing LPP1
-
rat2 fibroblasts transduced and overexpressed with LPP1 and mLPP1-GFP
-
rat2 fibroblasts transduced and overexpressed with LPP2, LPP2-GFP and mutant LPP2(R214K)-GFP
-
rat2 fibroblasts transduced and overexpressed with LPP3-GFP or myc-LPP3
-
separate overexpression of isozymes LPP1, LPP2, or LPP3 in HEK-293 cells reducing lysophosphatidic acid-stimulated p42/p44 MAPK activation
-
subcloning and expression of wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3)
-
transcript PAP2d_v1, cDNA library construction, DNA and amino acid sequence determination and analysis, genomic localization of the two splicing variants, expression patterns, expression in Escherichia coli strain DH5alpha
transcript PAP2d_v2, cDNA library construction, DNA and amino acid sequence determination and analysis, genomic localization of the two splicing variants, expression patterns, expression in Escherichia coli strain DH5alpha
transient and stable overexpression in HEK-293 cells, baculovirus vectors for recombinant expression of LPPs in cultured Sf9 cells
-
transient expression of lipin-1 in McA-RH7777 cells specifically influences the rate of incorporation of methionine into apolipoprotein B100, and stimulates its synthesis and secretion, overview
-
V5 epitope-tagged lipin-1 and lipin-2 expression plasmids, transiently transfected into Hepa 1–6 cells. Wild-type lipin-1A and lipin-2 and mutants lipin-1A-S724L and lipin-2-S731L expressed in HEK-293 cells
-
V5-tagged lipin-1alpha, lipin-1beta, lipin-2 and lipin-3 expression vectors. HEK-293A cells transfected with plasmids expressing V5-tagged proteins. HeLa cells transfected with expression plasmids for V5-lipin-1beta and CFP-SUMO-1 or their mutants. SH-SY5Y cells stably expressing pcDNA3, lipin-1alpha-V5 or lipin-1alpha-K566R/K596R-V5. Cerebrocortical neurons from embryonic day 17 rat embryos transfected either with V5-tagged lipin-1alpha or lipin-1beta, or with the corresponding double sumoylation site mutants
-
wild-type and mutant LPP1, LPP2, and LPP3 cDNAs subcloned into the pLNCX2 retroviral vector directly downstream of the human cytomegalovirus immediate-early promoter. Constructs transfected inot human dermal microvascular endothelial cells. HA-tagged wild-type LPP3 (pLNCX2-HA-WT-hLPP3) stably transfected into HEK-293 cells
-
wild-type transfected inot human dermal microvascular endothelial cells
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