Cloned (Comment) | Organism |
---|---|
isozyme LPPbeta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree | Arabidopsis thaliana |
isozyme LPPepsilon1, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation | Arabidopsis thaliana |
isozyme LPPepsilon2, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic ARabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation | Arabidopsis thaliana |
isozyme LPPgamma, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of isozyme LPPgamma in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation | Arabidopsis thaliana |
isozymes LPPdelta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a lppepsilon1lppepsilon2 double knock-out mutant shows no significant changes in lipid composition | Arabidopsis thaliana |
additional information | a lppgamma homozygous mutant is isolated only under ectopic overexpression of LPPgamma, suggesting that loss of LPPgamma may cause lethal effect on plant viability | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | about 75% inhibition at 20 mM, isozyme LPPgamma; about 95% inhibition at 20 mM, isozyme LPPepsilon1 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the homolog of cyanobacterial LPP, isozyme LPPepsilon1, is localized to chloroplasts and contains a plastidic transit peptide | Arabidopsis thaliana | 9507 | - |
chloroplast | the homolog of cyanobacterial LPP, isozyme LPPepsilon2, is localized to chloroplasts and contains a plastidic transit peptide | Arabidopsis thaliana | 9507 | - |
chloroplast | the homolog of cyanobacterial LPP, isozyme LPPgamma, is localized to chloroplasts and contains a plastidic transit peptide | Arabidopsis thaliana | 9507 | - |
additional information | isozyme LPPbeta contains no plastidic transit peptide | Arabidopsis thaliana | - |
- |
additional information | isozyme LPPdelta contains no plastidic transit peptide | Arabidopsis thaliana | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 50% inhibition at 10-20 mM, isozyme LPPepsilon2 | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23371 | - |
x * 23371, isozyme LPPdelta, sequence calculation | Arabidopsis thaliana |
25736 | - |
x * 25736, isozyme LPPgamma, sequence calculation | Arabidopsis thaliana |
30578 | - |
x * 30578, isozyme LPPepsilon1, sequence calculation | Arabidopsis thaliana |
31512 | - |
x * 31512, isozyme LPPepsilon2, sequence calculation | Arabidopsis thaliana |
46151 | - |
x * 46151, isozyme LPPdelta, sequence calculation | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidic acid + H2O | Arabidopsis thaliana | - |
1,2-sn-diacylglycerol + phosphate | - |
? | |
phosphatidic acid + H2O | Arabidopsis thaliana | the plastidic phosphatidic acid phosphatase dephosphorylates phosphatidic acid to yield diacylglycerol, which is a precursor for galactolipids, a primary and indispensable component of photosynthetic membranes | 1,2-sn-diacylglycerol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
This UniProt-ID has been deleted; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP | - |
Arabidopsis thaliana | Q0WUC2 | isozyme LPPdelta; ecotype Columbia-0 | - |
Arabidopsis thaliana | Q6NLA5 | isozyme LPPgamma; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP | - |
Arabidopsis thaliana | Q6NQL6 | isozyme LPPepsilon2; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP | - |
Arabidopsis thaliana | Q9SUW4 | isozyme LPPbeta; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP | - |
Purification (Comment) | Organism |
---|---|
chloroplast preparation from native and transgenic plants | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
shoot | - |
Arabidopsis thaliana | - |
shoot | high expression of isozyme LPPgamma | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidic acid + H2O | - |
Arabidopsis thaliana | 1,2-sn-diacylglycerol + phosphate | - |
? | |
phosphatidic acid + H2O | the plastidic phosphatidic acid phosphatase dephosphorylates phosphatidic acid to yield diacylglycerol, which is a precursor for galactolipids, a primary and indispensable component of photosynthetic membranes | Arabidopsis thaliana | 1,2-sn-diacylglycerol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23371, isozyme LPPdelta, sequence calculation | Arabidopsis thaliana |
? | x * 25736, isozyme LPPgamma, sequence calculation | Arabidopsis thaliana |
? | x * 30578, isozyme LPPepsilon1, sequence calculation | Arabidopsis thaliana |
? | x * 31512, isozyme LPPepsilon2, sequence calculation | Arabidopsis thaliana |
? | x * 46151, isozyme LPPdelta, sequence calculation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
lipid phosphate phosphatase | - |
Arabidopsis thaliana |
LPP | - |
Arabidopsis thaliana |
More | plastidic PAPs in Arabidopsis belong to a distinct lipid phosphate phosphatase LPP subfamily with prokaryotic origin | Arabidopsis thaliana |
PAP | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | isozyme LPPgamma, broad optimum | Arabidopsis thaliana |
7 | - |
isozyme LPPepsilon1 | Arabidopsis thaliana |
7 | - |
isozyme LPPepsilon2 | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | isozyme LPPepsilon1 | Arabidopsis thaliana |
5 | 10 | isozyme LPPepsilon2 | Arabidopsis thaliana |
5 | 10 | isozyme LPPgamma | Arabidopsis thaliana |