Cloned (Comment) | Organism |
---|---|
expression of Myc-tagged and/or FLAG-tagged LPP1, LLP2, and LLP3 in HEK-293 cells, overview | Mus musculus |
LPP1 wild-type or mutants inserted into vector pcDNA3.1 with a C-terminal Myc epitope tag or FLAG epitope tag. HEK-293 cells transfected with plasmid construct encoding catalytically deficient Myc-tagged R127K LPP1 and/or FLAG-tagged H223L LPP1 and/or FLAG-tagged and/or Myc-tagged wild-type | Cavia porcellus |
LPP2 and LPP3 inserted into vector pcDNA3.1 with a C-terminal Myc epitope tag or FLAG epitope tag and transiently transfected into HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H171L | site-directed mutagenesis of the C2 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
H223L | site-directed mutagenesis of the C3 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
H223L | mutated in C3 domain, catalytically deficient Myc-tagged R127K LPP1 and FLAG-tagged H223L LPP1 can form oligomers | Cavia porcellus |
K120R | site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
P128I | site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
R127K | site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
R127K | mutated in C1 domain, catalytically deficient Myc-tagged R127K LPP1 and FLAG-tagged H223L LPP1 can form oligomers | Cavia porcellus |
R217K | site-directed mutagenesis of the C3 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
S169T | site-directed mutagenesis of the C2 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | Myc-gpLPP1-FLAG-LPP2, FLAG-gpLPP1-Myc-LPP3 and Myc-LPP2-FLAG-LPP3 are co-localized at the plasma membrane and in a perinuclear region of HEK-293 cells | Homo sapiens | - |
- |
additional information | Myc-LPP1-FLAG-hLPP2 and FLAG-LPP1-Myc-hLPP3 are co-localized at the plasma membrane and in a perinuclear region of HEK-293 cells | Cavia porcellus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 | Cavia porcellus |
32000 | - |
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 | Cricetulus griseus |
32000 | - |
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
33000 | - |
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 | Cricetulus griseus |
33000 | - |
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
34000 | - |
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 | Cricetulus griseus |
34000 | - |
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
34500 | - |
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 | Cavia porcellus |
35000 | - |
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 | Cavia porcellus |
36000 | - |
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Cricetulus griseus | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO cell | - |
Cricetulus griseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | endogenous LPP2 and LPP3 form a complex | Mus musculus | ? | - |
? | |
phosphatidic acid + H2O | - |
Cavia porcellus | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
phosphatidic acid + H2O | - |
Homo sapiens | 1,2-diacyl-sn-glycerol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000-36000, recombinant Myc-tagged and/or FLAG-tagged LPP1 or LLP3, SDS-PAGE | Mus musculus |
? | x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 | Cavia porcellus |
? | x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 | Cricetulus griseus |
? | x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
More | LPP1 monomers might exist in equilibrium with oligomeric LPP1, the LPP1 monomer is inactive, endogenous LPP2 and LPP3 form a complex | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
lipid phosphate phosphatase | - |
Cricetulus griseus |
lipid phosphate phosphatase | - |
Cavia porcellus |
lipid phosphate phosphatase | - |
Mus musculus |
lipid phosphate phosphatase | - |
Homo sapiens |
LLP2 | - |
Mus musculus |
LPP1 | - |
Cavia porcellus |
LPP1 | - |
Mus musculus |
LPP2 | - |
Cricetulus griseus |
LPP2 | - |
Homo sapiens |
LPP3 | - |
Cricetulus griseus |
LPP3 | - |
Mus musculus |
LPP3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | catalytically deficient FLAG-tagged H223L LPP1 mutant can form an oligomer with wild-type LPP1, whereby wild-type LPP1 activity is preserved in the oligomer | Cavia porcellus |
physiological function | endogenous LPP2 and LPP3 form a complex. Endogenous LPP2 and LPP3 form homo- and hetero-oligomers, which differ in their subcellular localization and which may confer differing spatial regulation of phosphatidic acid and sphingosine 1-phosphate signalling | Homo sapiens |
physiological function | LPP1 forms both homo- and hetero-oligomers. Full catalytic activity is not required for oligomerization | Cavia porcellus |
physiological function | LPP2 forms a complex with the 32 kDa form of LPP3, but not with the 34 kDa form. LPP oligomers may regulate compartmentalized pools of sphingosine 1-phosphate and phosphatidic acid and contribute to the spatial signalling by these lipids within cells | Cricetulus griseus |