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Literature summary for 3.1.3.4 extracted from

  • Long, J.S.; Pyne, N.J.; Pyne, S.
    Lipid phosphate phosphatases form homo- and hetero-oligomers: catalytic competency, subcellular distribution and function (2008), Biochem. J., 411, 371-377.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of Myc-tagged and/or FLAG-tagged LPP1, LLP2, and LLP3 in HEK-293 cells, overview Mus musculus
LPP1 wild-type or mutants inserted into vector pcDNA3.1 with a C-terminal Myc epitope tag or FLAG epitope tag. HEK-293 cells transfected with plasmid construct encoding catalytically deficient Myc-tagged R127K LPP1 and/or FLAG-tagged H223L LPP1 and/or FLAG-tagged and/or Myc-tagged wild-type Cavia porcellus
LPP2 and LPP3 inserted into vector pcDNA3.1 with a C-terminal Myc epitope tag or FLAG epitope tag and transiently transfected into HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H171L site-directed mutagenesis of the C2 domain conserved amino acid residue leads to reduced activity of LPP1 Mus musculus
H223L site-directed mutagenesis of the C3 domain conserved amino acid residue leads to reduced activity of LPP1 Mus musculus
H223L mutated in C3 domain, catalytically deficient Myc-tagged R127K LPP1 and FLAG-tagged H223L LPP1 can form oligomers Cavia porcellus
K120R site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 Mus musculus
P128I site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 Mus musculus
R127K site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 Mus musculus
R127K mutated in C1 domain, catalytically deficient Myc-tagged R127K LPP1 and FLAG-tagged H223L LPP1 can form oligomers Cavia porcellus
R217K site-directed mutagenesis of the C3 domain conserved amino acid residue leads to reduced activity of LPP1 Mus musculus
S169T site-directed mutagenesis of the C2 domain conserved amino acid residue leads to reduced activity of LPP1 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information Myc-gpLPP1-FLAG-LPP2, FLAG-gpLPP1-Myc-LPP3 and Myc-LPP2-FLAG-LPP3 are co-localized at the plasma membrane and in a perinuclear region of HEK-293 cells Homo sapiens
-
-
additional information Myc-LPP1-FLAG-hLPP2 and FLAG-LPP1-Myc-hLPP3 are co-localized at the plasma membrane and in a perinuclear region of HEK-293 cells Cavia porcellus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 Cavia porcellus
32000
-
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 Cricetulus griseus
32000
-
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 Homo sapiens
33000
-
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 Cricetulus griseus
33000
-
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 Homo sapiens
34000
-
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 Cricetulus griseus
34000
-
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 Homo sapiens
34500
-
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 Cavia porcellus
35000
-
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 Cavia porcellus
36000
-
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 Homo sapiens

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Cricetulus griseus
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information endogenous LPP2 and LPP3 form a complex Mus musculus ?
-
?
phosphatidic acid + H2O
-
Cavia porcellus 1,2-diacyl-sn-glycerol + phosphate
-
?
phosphatidic acid + H2O
-
Homo sapiens 1,2-diacyl-sn-glycerol + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 31000-36000, recombinant Myc-tagged and/or FLAG-tagged LPP1 or LLP3, SDS-PAGE Mus musculus
? x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 Cavia porcellus
? x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 Cricetulus griseus
? x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 Homo sapiens
More LPP1 monomers might exist in equilibrium with oligomeric LPP1, the LPP1 monomer is inactive, endogenous LPP2 and LPP3 form a complex Mus musculus

Synonyms

Synonyms Comment Organism
lipid phosphate phosphatase
-
Cricetulus griseus
lipid phosphate phosphatase
-
Cavia porcellus
lipid phosphate phosphatase
-
Mus musculus
lipid phosphate phosphatase
-
Homo sapiens
LLP2
-
Mus musculus
LPP1
-
Cavia porcellus
LPP1
-
Mus musculus
LPP2
-
Cricetulus griseus
LPP2
-
Homo sapiens
LPP3
-
Cricetulus griseus
LPP3
-
Mus musculus
LPP3
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mus musculus

General Information

General Information Comment Organism
malfunction catalytically deficient FLAG-tagged H223L LPP1 mutant can form an oligomer with wild-type LPP1, whereby wild-type LPP1 activity is preserved in the oligomer Cavia porcellus
physiological function endogenous LPP2 and LPP3 form a complex. Endogenous LPP2 and LPP3 form homo- and hetero-oligomers, which differ in their subcellular localization and which may confer differing spatial regulation of phosphatidic acid and sphingosine 1-phosphate signalling Homo sapiens
physiological function LPP1 forms both homo- and hetero-oligomers. Full catalytic activity is not required for oligomerization Cavia porcellus
physiological function LPP2 forms a complex with the 32 kDa form of LPP3, but not with the 34 kDa form. LPP oligomers may regulate compartmentalized pools of sphingosine 1-phosphate and phosphatidic acid and contribute to the spatial signalling by these lipids within cells Cricetulus griseus