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Literature summary for 3.1.3.4 extracted from

  • Pillai, A.N.; Shukla, S.; Gautam, S.; Rahaman, A.
    Small phosphatidate phosphatase (TtPAH2) of Tetrahymena complements respiratory function and not membrane biogenesis function of yeast PAH1 (2017), J. Biosci., 42, 613-621 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-(DE3) cells Tetrahymena thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Tetrahymena thermophila

Organism

Organism UniProt Comment Textmining
Tetrahymena thermophila
-
-
-
Tetrahymena thermophila B2086
-
-
-
Tetrahymena thermophila CU428
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
Tetrahymena thermophila

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Tetrahymena thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidic acid + H2O
-
Tetrahymena thermophila 1,2-diacyl-sn-glycerol + phosphate
-
?
phosphatidic acid + H2O
-
Tetrahymena thermophila CU428 1,2-diacyl-sn-glycerol + phosphate
-
?
phosphatidic acid + H2O
-
Tetrahymena thermophila B2086 1,2-diacyl-sn-glycerol + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Tetrahymena thermophila

Synonyms

Synonyms Comment Organism
PAH2
-
Tetrahymena thermophila
PAP1
-
Tetrahymena thermophila

General Information

General Information Comment Organism
physiological function isoform PAH2 possesses the minimal function of phosphatidate phosphatase protein family in respiration. The enzyme is not essential for the normal growth and survival of Tetrahymena Tetrahymena thermophila