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3.1.1.4: phospholipase A2

This is an abbreviated version!
For detailed information about phospholipase A2, go to the full flat file.

Word Map on EC 3.1.1.4

Reaction

phosphatidylcholine
+
H2O
=
1-acylglycerophosphocholine
 +
a carboxylate

Synonyms

1-CysPrx, 1-cysteine peroxiredoxin, 14 kDa phospholipase A2, A2-IIA, Acanmyotoxin-1, acidic Asp49 phospholipase A2, acidic Ca2+-independent phospholipase A2, acidic phospholipase A2, ACS, adiponutrin, AdPLA, Agkistrotoxin, AgkTx-II, aiPLA2, alpha-type phospholipase A2, amdI1, Ammodytin I2, ammodytoxin A, APLA, APP-D-49, Arg49 phospholipase A2, Arg49 phospholipase A2 homologue, Asp49 basic myotoxic PLA2, Asp49 PLA2, Asp49-PLA2, ASPLA1, ASPLA10, ASPLA11, ASPLA12, ASPLA13, ASPLA14, ASPLA15, ASPLA16, ASPLA17, ASPLA2, ASPLA3, ASPLA4, ASPLA5, ASPLA6, ASPLA7, ASPLA8, ASPLA9, Ats-PLA2-alpha, AtsPLA2-alpha, AtsPLA2-gamma, ATX, BaltPLA2, basic Asp49 PLA2, Basic protein I/II, BaTX, BinTx-I, BinTx-II, BJ-PLA2, Bj-V, BJUPLA2, Bothropstoxin-I, BPI/BPII, BthTX, BthTX-1, BthTX-I, BthTX-II, bvPLA2, Ca+2-independent PLA2, Ca2+ independent PLA2, Ca2+-independent iPLA2, Ca2+-independent phospholipase A2, Ca2+-independent PLA2, CaI-PLA2, calcium-d-independent cytosolic PLA2, calcium-dependent cytosolic PLA2, calcium-independent group VIA iPLA2, calcium-independent phospholipase A2, Caudoxin, Cdt PLA2, class XIB phospholipase A2, ColTx-I, cPLA2, cPLA2 alpha, cPLA2-alpha, cPLA2-gamma, cPLA2-zeta2, cPLA2alpha, cPLA2beta, cPLA2delta, cPLA2epsilon, cPLA2zeta, cPm09, Cr-IV 1, Cro, crotapotin, crotoxin, CTX, cytoplasmic phospholipase A2, cytosolic calcium-dependent PLA2, cytosolic cPLA2, cytosolic gIVaPLA2, cytosolic group IVA cPLA2, cytosolic group IVa phospholipase A2, cytosolic phospholipase A2, cytosolic phospholipase A2 alpha, cytosolic phospholipase A2-alpha, cytosolic phospholipase A2alpha, cytosolic PLA2, cytotoxin ExoU, Daboxin P, DrK-aI, DrK-aII, DrK-bI, DrK-bII, DrPLA2, DsM-bI, DsM-S1, Enhancing factor, exoenzyme U, ExoU, ExoU-specific PLA2, GIA cobra venom PLA2, GIIA, GIIA PLA2, GIIA sPLA2, GIIC sPLA2, GIID, GIID sPLA2, GIIE, GIIE sPLA2, GIIF, GIIF sPLA2, GIII sPLA2, GIIIsPLA2, GIVA, GIVA cPLA2, GIVA phospholipase A2, GIVA PLA2, GIVD cPLA2delta, GIVF, Gln49-PLA2, group IA PLA2, Group IB phospholipase A2, group IB PLA2, group II snake venom phospholipase A2, Group IIA phospholipase A2, group IIA PLA2, group IIA secretory phospholipase A2, group IIA secretory PLA2, group IID secretory phospholipase A2, group III Glu62-phospholipase A2, group III PLA2, group III sPLA2, group IV cPLA2, group IVA cPLA2, group IVA cytosolic phospholipase A2, group IVA phospholipase A2, group IVA PLA2, group IVB phospholipase A2, Group V phospholipase A2, group V secreted phospholipase A2, Group VI phospholipase A2, Group VIA Ankyrin-1, Group VIA Ankyrin-2, group VIA calcium-independent phospholipase A, group VIA cPLA2, group VIA phospholipase A2, Group VIA PLA2, Group VIA-3, group X secreted phospholipase A2, GS2, GV sPLA2, GVI PLA2, GVIA, GVIA iPLA2, GVIA PLA2, GVIA-iPLA2, GVIIA PLA2, GX, GX sPLA2, GXII sPLA2, GXIIA, GXIII sPLA2, hGX sPLA2, hIBPLA2, hIIAPLA2, human group IB PLA2, human group IIA phospholipase A2, IB PLA2, immune-associated phospholipase A2, imperatoxin, intercro, IPLA-1, iPLA2, iPLA2-gamma, jerdoxin, lecithinase A, lipoprotein associated phospholipase A2, lipoprotein-associated phospholipase A2, lipoprotein-associated PLA2, LLPL, LM-PLA2-I, LM-PLA2-II, LmTX-I, LmTX-II, Lp-PLA2, Lpla2, Lys49 phospholipase A2 homologue, Lys49-phospholipase A2 homologue, Lys49-PLA2, lysophospholipase, lysosomal phospholipase A2, lysosomal PLA2, mAoPlaA, marine snail digestive phospholipase A2, megacin A-216, MiDCA1, milleporin-1, MjTX-II, More, MP-III 4R, mSDPL, MsPLA2-I, Muscarinic inhibitor, mycotoxin II, myotoxic Asp49-phospholipase A2, myotoxic phospholipase A2, Myotoxin, myotoxin I, NAJPLA-2A, NAJPLA-2B, NAJPLA-2C, natratoxin, neuropathy target esterase, neutral anticoagulant secretory phospholipase A2, Nigexine, NK-PLA2-I, NK-PLA2-II, NN-X-PLA2, NN-XI-PLA2, NN-XIa-PLA2, NND-IV-PLA2, Non-pancreatic secretory phospholipase A2, Notechis 11'2, Notexin, notexinII-1, NPLA, NPS-PLA2, OHV A-PLA2, OHV-APLA2, OPLA2, PAF acetyl hydrolase/oxidized lipid LpPLA2, PAF-AH, pancreatic phospholipase A2, pancreatic-type PLA2, patatin, peroxiredoxin 6, pgPLA 1a/pgPLA 2a, PhlA, phosphatidase, phosphatide 2-acylhydrolase, phosphatidolipase, Phosphatidylcholine 2-acylhydrolase, Phosphatidylcholine 2-acylhydrolase GIIC, Phosphatidylcholine 2-acylhydrolase GIID, Phosphatidylcholine 2-acylhydrolase GIIE, Phosphatidylcholine 2-acylhydrolase GIIF, Phosphatidylcholine 2-acylhydrolase GIII, Phosphatidylcholine 2-acylhydrolase GX, Phosphatidylcholine 2-acylhydrolase GXII, Phosphatidylcholine 2-acylhydrolase GXIII, phospholipase A, phospholipase A2, phospholipase A2 D49, phospholipase A2 gamma, Phospholipase A2 inhibitor, phospholipase A2 neurotoxin, phospholipase A2 PA-11, phospholipase A2alpha, phospholipase A2IValpha, phospholipase A2s, phospholipin, pkP5, Pla, PLA1, PLA2, PLA2 CM2, PLA2 neurotoxin, PLA2-10, PLA2-1B, PLA2-2A, PLA2-2C, PLA2-H, PLA2-I, PLA2-L, PLA2-V, PLA2-VI, PLA2-VII, PLA2alpha, PLA2G1B, PLA2G2D, Pla2g4a, Pla2g4b, PLA2G4C, PLA2G4D, PLA2G6, PLA2IID, PLA2III, PLA2IValpha, PLA2s, platelet activating factor acetyl hydrolase, platelet activating factor acetyl hydrolase/oxidized lipid LpPLA2, platelet-activating factor acetylhydrolase, platlet-activating factor acetylhydrolase, plpA, ppPLA2, Prdx6, promutoxin, PrTX-1, PrTX-I, PrTX-III, Pt-PLA1, Pt-PLA2, R49 PLA2, RVV acidic PLA2-I, RVV-7, RVVA-PLA2-I, SCO1048 protein, Scol/Pla, secreted group IID phospholipase A2, secreted human phospholipase A2, secreted phospholipase A2, secreted phospholipaseA2 neurotoxin, secreted phospholipases A2, secreted PLA2, secreted sPLA2, secretory Ca2+-dependent PLA2, secretory phospholipase, secretory phospholipase A2, secretory phospholipase A2 group IIA, secretory phospholipase A2 type IB, secretory phospholipase A2 type IIA, secretory phospholipase A2-alpha, secretory PLA2s, Secretory-type PLA, stroma-associated homolog, snake presynoptic phospholipase A2 neurotoxin, SPAN, specific phospholipase A2, sPLA(2), sPLA(2)-IID, sPLA(2)-IIE, sPLA(2)-IIF, sPLA-V, sPLA2, sPLA2 GIB, sPLA2 GIIA, sPLA2 IB, sPLA2 type IIA, sPLA2(IIA), sPLA2-IB, sPLA2-IIA, sPLA2-IID, sPLA2-IIE, sPLA2-IIF, sPLA2-V, sPLA2-X, sPLA2:, sPLA2IB2, svPLA2, taipoxin, textilotoxin, thrombin inhibitor from Naja haje, TI-Nh, TMV-K49, toxin I, Toxin VI, Toxin VI:5, TPP, trimorphin, TTS-2.2, Tx-1, TX-I, type III cytotoxin, type VIIA PLA2, zhaoermiatoxin

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.4 phospholipase A2

Specific Activity

Specific Activity on EC 3.1.1.4 - phospholipase A2

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000006
-
isoform cPLA2delta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.00001
-
cytosolic colon mucosa PLA2, substrate: 1-palmitoyl-2-oleolylphosphatidylcholine
0.0000115
-
particulate associated hormonally regulated PLA2, substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine, mixed micelles with Triton X-100
0.000015
0.000019
-
cytosolic colon mucosa PLA2, substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine
0.00002
-
microsomal colon mucosa PLA2, substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine
0.000021
-
isoform cPLA2epsilon, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.00004
-
isoform cPLA2epsilon, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.0000538
-
particulate associated hormonally regulated PLA2, substrate: micellar 1-palmitoyl-2-arachidonylphosphatidylcholine
0.00006
-
isoform cPLA2delta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.000145
-
isoform cPLA2epsilon, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.00021
-
isoform cPLA2delta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.000245
-
isoform cPLA2beta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.000295
-
isoform cPLA2beta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.0003
0.0011
-
isoform cPLA2alpha, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.0014
-
oleate labelled Escherichia coli membrane
0.0016
-
arachidonate labelled Escherichia coli membrane
0.0021
-
arachidonate labelled Escherichia coli membrane
0.0023
-
oleate labelled Escherichia coli membrane
0.00245
-
isoform cPLA2zeta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.0056
-
-
0.0062
-
sigmoid colon mucosa, substrate: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
0.0069
-
-
0.0071
-
cecal mucosa, substrate: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
0.0072
-
ascending colon mucosa, substrate: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
0.0078
-
transverse colon mucosa, substrate: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
0.0092
-
descending colon mucosa, substrate: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
0.0106
1-palmitoyl-2-arachidonylphosphocholine as substrate
0.0156
1-stearoyl-2-arachidonylphosphocholine as substrate
0.01587
pH 8.0, 37°C
0.0172
-
substrate: 1-acyl-arachidonoyl-sn-glycero-3-phosphoethanolamine
0.0192
-
substrate: phosphatidylcholine
0.0225
0.027
-
acidic iPLA2, substrate: 1-O-hexadecyl-2-arachidonoyl-glycero-3-phosphocholine
0.031
-
MiPLA-3 isoform
0.0379
-
substrate: phosphatidylethanolamine
0.04
-
renatured enzyme, substrate: 1,2-dipalmitoyl-phosphatidylcholine
0.05
-
isoform cPLA2gamma, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.0509
-
ileal mucosa, substrate: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
0.058
-
isoform cPLA2gamma, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.06
-
isoform cPLA2zeta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.0609
-
substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine
0.064
-
acidic iPLA2, substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine or 1,2-dipalmitoyl-phosphatidylcholine
0.065
-
acidic iPLA2, substrate: oxidized 1-palmitoyl-2-arachidonylphosphatidylcholine
0.08
-
acidic iPLA2, pH 4.0, Ca2+-free medium, substrate: 1,2-dipalmitoyl-phosphatidylcholine
0.12
-
pancreas, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate
0.121
-
purified enzyme
0.126
-
isoform cPLA2alpha, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.14
-
substrate 1-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine
0.145
-
isoform cPLA2alpha, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.2
-
extraction at pH 8.5, in 10 mM Tris/HCl buffer, 10 mM CaCl2, 0.15 M NaCl
0.211
-
pancreas, substrate: 1-O-hexadecyl-oleolyl-sn-glycero-3-phosphocholine
0.23
-
isoform cPLA2alpha, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.259
-
substrate: 1-acyl-2-arachidonoyl-glycerophosphocholine
0.3
-
crude enzyme, in 10 mM Tris-HCl, 10 mM CaCl2 and 100 mM NaCl, pH 8.0, at 37°C
0.347
-
crude venom preparation
0.353
-
MiPLA-4 isoform
0.365
-
isoform cPLA2beta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.3695
-
isoform cPLA2zeta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C
0.372
-
isoform cPLA2zeta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.381
-
isoform cPLA2beta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C
0.4
-
substrate: phosphatidylcholine
0.45
-
antrum, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate
0.51
-
substrate: standardized commercial phospholipid emulsion
0.56
-
native enzyme, substrate: 1,2-dipalmitoyl-phosphatidylcholine
0.6
-
iPLA2 in a 30000fold purification, substrate: 1-stearoyl-2-arachidonoylphosphatidylcholine
0.72
-
crude extract, at 50°C and pH 8.5, with phosphatidylcholine liposomes as a substrate in the presence of 4 mM sodium taurodeoxycholate and 6 mM CaCl2
0.837
-
MiPLA-2 isoform
0.847
1 - 10
-
phospholipid/Triton X-100: 1/2,1/3,und 1/4, phospholipid concentration up to 30 mM
1.05
-
substrate: phosphatidylcholine
1.1
-
isoform Tpu-E6b, presence of Triton X-100, 37°C, pH 7.4
1.6
-
PLA2 zymogen, substrate: egg yolk emulsion
1.8
-
substrate: 1-palmitoyl-2-linoleoylphosphatidylcholine
101.2
-
isoenzyme NK-PLA2-I, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C
1030
-
gastric mucosa, substrate: 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine
1034
-
crude venom, pH and temperature not specified in the publication
1050
-
10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42
109
-
1,2-dinonanoyl-sn-glycero-3-phosphocholine as substrate
11
-
isoform Tpu-E6a, presence of deoxycholate, 37°C, pH 7.4
11.7
-
isoform Tbo-E6, presence of deoxycholate, 37°C, pH 7.4
110
-
recombinant enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine
1129
-
substrate: 1,2-diacyl-phosphatidylcholine
1140
-
substrate: egg yolk emulsion
11400
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine
1143
-
isoform G-2 PLA2
1150
-
substrate: egg yolk emulsion, PLA2 isoenzyme
118
-
substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine
1180
-
substrate: egg yolk emulsion, PLA2 isoenzyme
119000
purified wild-type enzyme
12.6
-
substrate: phosphatidylcholine
1220
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol
123
-
isoform NN-XI-PLA2, 37°C
124
-
substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine
125
-
substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine
127
-
1,2-diocytanoyl-sn-glycero-3-phosphocholine as substrate
1270
-
active PLA2, substrate: egg yolk emulsion
1293
-
isoform G-3 PLA2
1294
-
isoform G-1 PLA2
1300
-
egg yolk lipoprotein assay
133
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C
138
-
isoform NN-XIa-PLA2, 37°C
14.4
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dioctanoyl-sn-glycero-3-phosphocholine
1450
-
substrate: phosphatidylcholine
15
-
4-nitrophenylcaprate as substrate
15.3
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dioleoyl-sn-glycero-3-phosphorac-(1-glycerol)
15.6
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
150
-
5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.36
1500
-
de-/renatured acidic PLA2, PC for substrate
153.9
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C
155
-
substrate: dioleolyl-sn-phosphatidylethanolamine
159.9
-
purified enzyme, pH and temperature not specified in the publication
16.5
-
purified recombinant AtsPLA2-alpha, substrate 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
16.7
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dioleoyl-sn-glycero-3-phosphocholine
1600
-
de-/renatured basic-acidic PLA2, PA for substrate
16000
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol
162
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine
1648
-
pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, isoenzyme of e-amidinated PLA, 0.71 mM Ca2+
1670
-
phospholipid/Triton X-100: 1/3
1693
-
pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.42 mM Ca2+
17
-
native enzyme
17.8
-
purified recombinant AtsPLA2-alpha, substrate 1-oleoyl-2-stearoyl-sn-glycero-3-phosphocholine
171
-
substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine
172
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine
1750
-
de-/renatured basic PLA2, PA for substrate
178.4
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C
18
-
purified recombinant AtsPLA2-alpha, substrate 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine
180
-
250fold purified enzyme, at 50°C and pH 8.5, with phosphatidylcholine liposomes as a substrate in the presence of 4 mM sodium taurodeoxycholate and 6 mM CaCl2
19.5
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dilinoleoyl-sn-glycero-3-phosphocholine
2
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-arachidonoylphosphatidylcholine
2.08
25°C, pH not specified in the publication
20
-
fundus, substrate: mixed micelles of 1-palmitoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine and deoxycholate
20.6
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
202.3
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C
2038
-
pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.64 mM Ca2+
208
-
substrate: dioleolyl phosphatidylglycerol
210
-
substrate: dioleolyl-sn-phosphatidylethanolamine
2100
-
phospholipid/Triton X-100: 1/2
2170
-
pH8, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, iso-AMPA, 0.30 mM Ca2+
218.4
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C
220.5
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C
221
-
natural enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine
229.9
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C
2312
-
purified enzyme, pH and temperature not specified in the publication
243.1
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C
249
-
90°C, pH 8.0
25
-
substrate: dioleolyl-sn-phosphatidylcholine
25.3
-
purified recombinant AtsPLA2-alpha, substrate 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine
27.3
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dimyristoyl-sn-glycero-3-phosphocholine
27.7
-
pH 7.5, 22°C
270
-
isoform SIIISPIIIA
2725
-
pH8, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 2.0 mM Ca2+
279
-
isoform SIIISPIIB
29.3
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dilauroyl-sn-glycero-3-phosphocholine
2910
-
pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 8.1 mM Ca2+
295
-
substrate: dioleolyl-sn-phosphatidylethanolamine
3
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-oleolylphosphatidylcholine
3.13
-
isoform Tpu-E6b, presence of deoxycholate, 37°C, pH 7.4
3.22
-
substrate: 1,2-dipalmitoyl-phosphatidylcholine
30
-
substrate: 1,2-dipalmitoyl-phosphatidylcholine
306
-
purified PLA2 from ileal mucosa, substrate: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
3150
-
substrate: phosphatidylcholine
320
-
substrate: dioleolyl-sn-phosphatidylcholine
3235
-
substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine
3250
-
substrate: phosphatidylcholine
33
construct A (Leu-1 mutated to an alanine) and construct B (extra glycine inserted between the start methionine and Leu-1)
330
-
freshly dialyzed PLA2
34
-
pH 7.5
35
-
HSD-PLA2, 21fold purification, platelet activating factor for substrate
35.5
-
purified recombinant AtsPLA2-alpha, substrate 1,2-didecanoyl-sn-glycero-3-phosphocholine
37.08
Bothrops spp.
-
BthTX-II
38 - 61
-
enzyme from intervertebral disc
383
-
5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67
4.3
-
iPLA2 in a 30000fold purification, substrate: 1,2-dipalmitoyl-phosphatidylcholine
4.5
-
isoform Tbo-G6D49, presence of deoxycholate, 37°C, pH 7.4
4.65
-
isoform Tpu-G6D49, presence of deoxycholate, 37°C, pH 7.4
4.725
-
substrate: phosphatidylcholine
400
-
2000fold purified enzyme, at pH 9.5, 37°C, in the presence of 1 mM sodium taurodeoxycholate, 4 mM CaCl2 with phosphatidylcholine as substrate
408.3
-
after 1361.7fold purification, in 10 mM Tris-HCl, 10 mM CaCl2 and 100 mM NaCl, pH 8.0, at 37°C
410
-
substrate: dioleolyl-sn-phosphatidylcholine
460
-
7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67
4650
-
substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine
500
-
de-/renatured basic PLA2, PC for substrate
5000
-
substrate: phosphatidylethanolamine
520
-
10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67
530
-
substrate: dioleolyl phosphatidylglycerol
57
-
substrate: dimyristoyl-sn-phosphatidylcholine
573
-
pH 8, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.013 mM Ca2+
576
-
5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0
6.5
-
isoform Tpu-E6a, presence of Triton X-100, 37°C, pH 7.4
600
-
purified enzyme
615
-
substrate: dioleolyl phosphatidylglycerol
62000
-
pH 8.0, 30°C
628.1
purified recombinant His-tagged Lp-PLA2
63
-
in 20 mM HEPES buffer, 20 mM NaCl, 1 mM CaCl2, pH and temperature not specified in the publication
637.9
purified native Lp-PLA2
6410
-
substrate: egg yolk suspension
650
-
de-/renatured basic-acidic PLA2, PC for substrate
6671
-
pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.79 mM Ca2+
68
-
substrate: dimyristoyl-sn-phosphatidylcholine
69.2
-
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C
69.3
-
enzyme from crude venom, pH and temperature not specified in the publication
7.9
-
isoform Tbo-E6, presence of Triton X-100, 37°C, pH 7.4
74.3
-
isoform Tpu-E6c, presence of Triton X-100, 37°C, pH 7.4
741
-
5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42
76.3
-
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C
775
-
7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0
79
-
isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C
8.3
-
isoform Tbo-G6D49, presence of Triton X-100, 37°C, pH 7.4
8.56
-
substrate 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
80
-
substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine
800
-
isoenzyme b-PLA2, substrate: egg yolk emulsion
804
-
10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0
81
-
substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine
82
-
5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.20
840
-
purified enzyme
870
-
7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42
8870
-
substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine
89.3
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C
9.9
-
purified recombinant AtsPLA2-alpha, substrate 1,2-dioleoyl-sn-glycero-3-phosphoserine
9300
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol
933
-
5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 2.0
950
purified mutant enzyme
96.3
-
isoenzyme NK-PLA2-II, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C
97
-
isoform SIIISPIIIB
additional information