Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory to both isoenzymes: p-methylsulfonylfluoride, tosyl-L-phenylalaninchlormethylketon, N-bromosuccinamide, tosyl-L-lysinechlormethylketon | Naja kaouthia | |
p-bromophenacylbromide | i.e. pBPB, 3.3 mM, 95% inhibition of isoenzyme NK-PLA2-I, 89% inhibition of isoenzyme NK-PLA2-II | Naja kaouthia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13100 | - |
PAGE | Naja kaouthia |
13346 | - |
1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS | Naja kaouthia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Naja kaouthia | both isoenzymes NK-PLA2-I and NK-PLA2-II cause significantly more damage to mitochondrial membranes as compared to erythrocyte membranes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Naja kaouthia | - |
isoenzymes NK-PLA2-I and NK-PLA2-II | - |
Purification (Comment) | Organism |
---|---|
isoenzyme NK-PLA2-II | Naja kaouthia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Naja kaouthia | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
61 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C | Naja kaouthia |
69.2 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
76.3 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C | Naja kaouthia |
79 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C | Naja kaouthia |
89.3 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C | Naja kaouthia |
96.3 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
101.2 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
133 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C | Naja kaouthia |
153.9 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C | Naja kaouthia |
178.4 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
202.3 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C | Naja kaouthia |
218.4 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C | Naja kaouthia |
220.5 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C | Naja kaouthia |
229.9 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
243.1 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C | Naja kaouthia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | both isoenzymes NK-PLA2-I and NK-PLA2-II cause significantly more damage to mitochondrial membranes as compared to erythrocyte membranes | Naja kaouthia | ? | - |
? | |
additional information | isoenzyme NK-PLA2-I does not discriminate between saturated and unsaturated fatty acids whereas isoenzyme NK-PLA2-II shows a preference for unsaturated fatty acids | Naja kaouthia | ? | - |
? | |
phosphatidylcholine + H2O | preferred substrate for both isoenzymes, NK-PLA2-I and NK-PLA2-II | Naja kaouthia | 1-acylglycerophosphocholine + fatty acid | - |
? | |
phosphatidylethanolamine + H2O | - |
Naja kaouthia | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
phosphatidylserine + H2O | - |
Naja kaouthia | 1-acylglycerophosphoserine + fatty acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS | Naja kaouthia |
More | N-terminal amino acid sequence is identical for both isoenzymes NK-PLA2-I and NK-PLA2-II | Naja kaouthia |
Synonyms | Comment | Organism |
---|---|---|
NK-PLA2-I | - |
Naja kaouthia |
NK-PLA2-II | - |
Naja kaouthia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
45 min, isoenzyme NK-PLA2-I, 50% residual activity, isoenzyme NK-PLA2-II, 43% residual activity | Naja kaouthia |