Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Bothrocophias hyoprora | |
Mg2+ | - |
Bothrocophias hyoprora | |
Mn2+ | - |
Bothrocophias hyoprora | |
Zn2+ | - |
Bothrocophias hyoprora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.96 | - |
4-nitro-3-(octanoyloxy) benzoic acid | at pH 8.0, 37°C | Bothrocophias hyoprora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on Ca2+, full activity at 10 mM | Bothrocophias hyoprora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14249 | - |
x * 14249, MALDI-TOF mass spectrometry | Bothrocophias hyoprora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrocophias hyoprora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gamma-Bondapack C18 column chromatography | Bothrocophias hyoprora |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
crude enzyme, in 10 mM Tris-HCl, 10 mM CaCl2 and 100 mM NaCl, pH 8.0, at 37°C | Bothrocophias hyoprora |
408.3 | - |
after 1361.7fold purification, in 10 mM Tris-HCl, 10 mM CaCl2 and 100 mM NaCl, pH 8.0, at 37°C | Bothrocophias hyoprora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitro-3-(octanoyloxy) benzoic acid + H2O | - |
Bothrocophias hyoprora | 3-hydroxy-4-nitrobenzoate + octanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 14249, MALDI-TOF mass spectrometry | Bothrocophias hyoprora |
Synonyms | Comment | Organism |
---|---|---|
myotoxic phospholipase A2 | - |
Bothrocophias hyoprora |
PLA2 | - |
Bothrocophias hyoprora |
TX-I | - |
Bothrocophias hyoprora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | - |
Bothrocophias hyoprora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bothrocophias hyoprora |
General Information | Comment | Organism |
---|---|---|
physiological function | ex vivo, whole venom and TX-I phospholipases A2 cause blockade of the neuromuscular transmission in young chick biventer cervicis preparations similar to other isolated snake venom toxins from the Bothrops genus. In vivo, both induce local myotoxicity and systemic interleukin-6 response upon intramuscular injection, additionally, induce moderate footpad edema. In vitro, both induce low cytotoxicity in skeletal muscle myoblasts, however TX-I phospholipases A2 is able to lyse myotubes | Bothrocophias hyoprora |