Literature summary for 3.1.1.4 extracted from

Karray, A.; Frikha, F.; Ben Bacha, A.; Ben Ali, Y.; Gargouri, Y.; Bezzine, S.
Biochemical and molecular characterization of purified chicken pancreatic phospholipase A2 (2009), FEBS J., 276, 4545-4554.

Search for more literature for 3.1.1.4

Activating Compound

Activating Compound	Comment	Organism	Structure
sodium taurodeoxycholate	maximum phospholipase activity at 1 mm sodium taurodeoxycholate. Displays 50% of its maximum activity in the absence of bile salt dependent	Gallus gallus

Cloned(Commentary)

Cloned (Comment)	Organism
ligated into PCR-Blunt vector and transformed into Escherichia coli DH5alpha cells	Gallus gallus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	essential for both catalysis and enzyme binding to the substrate. The catalytic Ca2+ may be coordinated in a tetrahedral environment formed by the oxygen atoms of Tyr28, Gly30, Gly32 and Asp49. Second Ca2+-binding site is conserved and is formed by four residues: Glu71, Ile72, Asn89, and Glu92. Optimal Ca2+ concentration is about 4 mM	Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14000	-	gel filtration	Gallus gallus
14170	-	calculated from sequence	Gallus gallus

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by ammonium sulfate and ethanol precipitation, followed by gel filtration and gel filtration, to homogeneity, with a yield of 16%, 2000fold	Gallus gallus

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Gallus gallus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.2	-	extraction at pH 8.5, in 10 mM Tris/HCl buffer, 10 mM CaCl2, 0.15 M NaCl	Gallus gallus
400	-	2000fold purified enzyme, at pH 9.5, 37°C, in the presence of 1 mM sodium taurodeoxycholate, 4 mM CaCl2 with phosphatidylcholine as substrate	Gallus gallus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphatidylcholine + H2O	in the presence of substrate, the enzyme binds to the interface as an active form. This crucial step is mediated by a planar surface region of the protein. The corresponding hydrophobic surface involves hydrophobic residues (Leu2, Trp3, Phe19, Leu20, Leu31, Ile64, Leu65, Tyr69, Ile72, Tyr75, and Leu118), hydrophobic surface involved in lipid binding increases	Gallus gallus	1-acylglycerophosphocholine + a carboxylate	-	?

Subunits

Subunits	Comment	Organism
monomer	non-glycosylated protein, SDS-PAGE	Gallus gallus

Synonyms

Synonyms	Comment	Organism
group IB PLA2	-	Gallus gallus
phospholipase A2	-	Gallus gallus
PLA2	-	Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	with phosphatidylcholine as substrate in the presence of 4 mM Ca2+ and 1 mM sodium taurodeoxycholate	Gallus gallus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
37	40	loses ca. 80% of its activity after 20 min of incubation at 40°C	Gallus gallus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	with phosphatidylcholine as substrate in the presence of 4 mM Ca2+ and 1 mM sodium taurodeoxycholate	Gallus gallus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	9.5	-	Gallus gallus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	-	is inactivated after incubation for 30 min at pH values lower than 4	Gallus gallus

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Release 2023.1 (January 2023)