Application | Comment | Organism |
---|---|---|
food industry | PLA2 stability in the presence of organic solvents, as well as in acidic and alkaline pH and at high temperature makes it a good candidate for its application in food industry | Dasyatis pastinaca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
phosphatidylcholine | at pH 8.5 and 40°C | Dasyatis pastinaca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
1 * 14000, SDS-PAGE | Dasyatis pastinaca |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 97% and 93% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
Acetone | 95% and 92% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
acetonitrile | 105% activity at 50% (v/v) after 1 h and 2 h incubation | Dasyatis pastinaca |
Ethanol | 116% and 100% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
Methanol | 98% and 95% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dasyatis pastinaca | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Dasyatis pastinaca |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ethanol fractionation, Mono-S Sepharose column chromatography, Sephadex G-50 gel filtration, RP-HPLC C-8 column chromatography, and Mono-Q column chromatography | Dasyatis pastinaca |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Dasyatis pastinaca | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
750 | - |
purified enzyme, in the presence of 4 mM sodium taurodeoxycholate and 8 mM CaCl2 using phosphatidylcholine as substrate, at pH 8.0 and 40°C | Dasyatis pastinaca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Dasyatis pastinaca | 1-acylglycerophosphocholine + a carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 14000, SDS-PAGE | Dasyatis pastinaca |
Synonyms | Comment | Organism |
---|---|---|
pancreatic-type PLA2 | - |
Dasyatis pastinaca |
PLA2 | - |
Dasyatis pastinaca |
sPLA2-IB | - |
Dasyatis pastinaca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Dasyatis pastinaca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
187 | - |
phosphatidylcholine | at pH 8.5 and 40°C | Dasyatis pastinaca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Dasyatis pastinaca |