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Literature summary for 3.1.1.4 extracted from
- Cloning, expression, and purification of lipoprotein-associated phospholipase A2 in Pichia pastoris (2006), Mol. Biotechnol., 33, 29-36.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | Lp-PLA2 is proposed as a promising target for drug discovery | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Lp-PLA2 gene, cloning from differentiated THP-1 cells, DNA and amino acid sequence determination and anaylsis, expression of C-terminally His6-tagged enzyme in Pichia pastoris strain GS115 and Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| SB435495 | specific inhibition of isozyme Lp-PLA2, used for inhibitor screening | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0137 | - |
1-hexadecyl-2-acetyl-sn-glycerol-3-phosphocholine | pH 7.4, 37°C, native Lp-PLA2 | Homo sapiens | |
| 0.0142 | - |
1-hexadecyl-2-acetyl-sn-glycerol-3-phosphocholine | pH 7.4, 37°C, recombinant His-tagged Lp-PLA2 | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hexadecyl-2-acetyl-sn-glycerol-3-phosphocholine + H2O | Homo sapiens | i.e. platelet-activating factor | 1-hexadecyl-sn-glycerol-3-phosphocholine + acetate | - |
? | |
| additional information | Homo sapiens | the lipoprotein-associated PLA2 plays a crucial role in atherosclerosis, and is proposed as a promising target for drug discovery | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q13093 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged Lp-PLA2 from Pichia pastoris by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| macrophage | - |
Homo sapiens | - |
| mast cell | - |
Homo sapiens | - |
| monocyte | - |
Homo sapiens | - |
| T-lymphocyte | - |
Homo sapiens | - |
| THP-1 cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 628.1 | - |
purified recombinant His-tagged Lp-PLA2 | Homo sapiens |
| 637.9 | - |
purified native Lp-PLA2 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hexadecyl-2-acetyl-sn-glycerol-3-phosphocholine + H2O | i.e. platelet-activating factor | Homo sapiens | 1-hexadecyl-sn-glycerol-3-phosphocholine + acetate | - |
? | |
| additional information | the lipoprotein-associated PLA2 plays a crucial role in atherosclerosis, and is proposed as a promising target for drug discovery | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipoprotein-associated phospholipase A2 | - |
Homo sapiens |
| Lp-PLA2 | - |
Homo sapiens |
| More | cf. EC 3.1.1.47, Lp-PLA2 is a member of the phospholipase A2 superfamily and belongs to the VII phospholipase A2 subgroup | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0159 | - |
SB435495 | isozyme Lp-PLA2 | Homo sapiens | |
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