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Literature summary for 3.1.1.4 extracted from

  • Almeida, J.R.; Resende, L.M.; Silva, A.G.; Ribeiro, R.I.; Stabeli, R.G.; Soares, A.M.; Calderon, L.A.; Marangoni, S.; Da Silva, S.L.
    Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from Crotalus oreganus lutosus (Great Basin rattlesnake) snake venom (2016), Toxicon, 117, 1-12 .
    View publication on PubMed
Search for more literature for 3.1.1.4

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 10 mM, notable inhibition even in the presence of 10 mM Ca2+ Crotalus oreganus lutosus
Zn2+ 10 mM, notable inhibition even in the presence of 10 mM Ca2+ Crotalus oreganus lutosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.09
-
 4-nitro-3-octanoyloxy benzoic acid pH 8.0, 37°C Crotalus oreganus lutosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activity is dependent on Ca2+ (10 mM). ColTx-I shows low activity when the enzymatic assay is performed using only the Tris-HCl buffer (pH 8.0) with no added calcium. None of the other tested ions (Cd2+, Mg2+, Mn2+, Zn2+) activates the purified toxin Crotalus oreganus lutosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 SDS-PAGE Crotalus oreganus lutosus
14145
-
 calculated from sequence Crotalus oreganus lutosus

Organism

Organism UniProt Comment Textmining
Crotalus oreganus lutosus C0HK05
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Crotalus oreganus lutosus

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Crotalus oreganus lutosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01587
-
 pH 8.0, 37°C Crotalus oreganus lutosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitro-3-octanoyloxy benzoic acid + H2O
-
 Crotalus oreganus lutosus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 14000, SDS-PAGE Crotalus oreganus lutosus
monomer 1 * 14145, calculated from sequence Crotalus oreganus lutosus

Synonyms

Synonyms Comment Organism
ColTx-I
-
 Crotalus oreganus lutosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Crotalus oreganus lutosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 25°C: about 65% of maximal activity, 60°C: about 55% of maximal activity Crotalus oreganus lutosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Crotalus oreganus lutosus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 10% of maximal activity, pH 9.0: about 12.5% of maximal activity Crotalus oreganus lutosus

General Information

General Information Comment Organism
physiological function phospholipases A2 (PLA2s) play key roles in the pathogenesis of the local tissue damage characteristic of crotaline and viperine snake envenomations. ColTx-I induces a complex series of degenerative events associated with edema, inflammatory infiltrate and skeletal muscle necrosis. IT plays a relevant role in Crotalus oreganus lutosus envenomation Crotalus oreganus lutosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01597
-
 4-nitro-3-octanoyloxy benzoic acid pH 8.0, 37°C Crotalus oreganus lutosus