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3.1.1.4: phospholipase A2

This is an abbreviated version!
For detailed information about phospholipase A2, go to the full flat file.

Word Map on EC 3.1.1.4

Reaction

phosphatidylcholine
+
H2O
=
1-acylglycerophosphocholine
 +
a carboxylate

Synonyms

1-CysPrx, 1-cysteine peroxiredoxin, 14 kDa phospholipase A2, A2-IIA, Acanmyotoxin-1, acidic Asp49 phospholipase A2, acidic Ca2+-independent phospholipase A2, acidic phospholipase A2, ACS, adiponutrin, AdPLA, Agkistrotoxin, AgkTx-II, aiPLA2, alpha-type phospholipase A2, amdI1, Ammodytin I2, ammodytoxin A, APLA, APP-D-49, Arg49 phospholipase A2, Arg49 phospholipase A2 homologue, Asp49 basic myotoxic PLA2, Asp49 PLA2, Asp49-PLA2, ASPLA1, ASPLA10, ASPLA11, ASPLA12, ASPLA13, ASPLA14, ASPLA15, ASPLA16, ASPLA17, ASPLA2, ASPLA3, ASPLA4, ASPLA5, ASPLA6, ASPLA7, ASPLA8, ASPLA9, Ats-PLA2-alpha, AtsPLA2-alpha, AtsPLA2-gamma, ATX, BaltPLA2, basic Asp49 PLA2, Basic protein I/II, BaTX, BinTx-I, BinTx-II, BJ-PLA2, Bj-V, BJUPLA2, Bothropstoxin-I, BPI/BPII, BthTX, BthTX-1, BthTX-I, BthTX-II, bvPLA2, Ca+2-independent PLA2, Ca2+ independent PLA2, Ca2+-independent iPLA2, Ca2+-independent phospholipase A2, Ca2+-independent PLA2, CaI-PLA2, calcium-d-independent cytosolic PLA2, calcium-dependent cytosolic PLA2, calcium-independent group VIA iPLA2, calcium-independent phospholipase A2, Caudoxin, Cdt PLA2, class XIB phospholipase A2, ColTx-I, cPLA2, cPLA2 alpha, cPLA2-alpha, cPLA2-gamma, cPLA2-zeta2, cPLA2alpha, cPLA2beta, cPLA2delta, cPLA2epsilon, cPLA2zeta, cPm09, Cr-IV 1, Cro, crotapotin, crotoxin, CTX, cytoplasmic phospholipase A2, cytosolic calcium-dependent PLA2, cytosolic cPLA2, cytosolic gIVaPLA2, cytosolic group IVA cPLA2, cytosolic group IVa phospholipase A2, cytosolic phospholipase A2, cytosolic phospholipase A2 alpha, cytosolic phospholipase A2-alpha, cytosolic phospholipase A2alpha, cytosolic PLA2, cytotoxin ExoU, Daboxin P, DrK-aI, DrK-aII, DrK-bI, DrK-bII, DrPLA2, DsM-bI, DsM-S1, Enhancing factor, exoenzyme U, ExoU, ExoU-specific PLA2, GIA cobra venom PLA2, GIIA, GIIA PLA2, GIIA sPLA2, GIIC sPLA2, GIID, GIID sPLA2, GIIE, GIIE sPLA2, GIIF, GIIF sPLA2, GIII sPLA2, GIIIsPLA2, GIVA, GIVA cPLA2, GIVA phospholipase A2, GIVA PLA2, GIVD cPLA2delta, GIVF, Gln49-PLA2, group IA PLA2, Group IB phospholipase A2, group IB PLA2, group II snake venom phospholipase A2, Group IIA phospholipase A2, group IIA PLA2, group IIA secretory phospholipase A2, group IIA secretory PLA2, group IID secretory phospholipase A2, group III Glu62-phospholipase A2, group III PLA2, group III sPLA2, group IV cPLA2, group IVA cPLA2, group IVA cytosolic phospholipase A2, group IVA phospholipase A2, group IVA PLA2, group IVB phospholipase A2, Group V phospholipase A2, group V secreted phospholipase A2, Group VI phospholipase A2, Group VIA Ankyrin-1, Group VIA Ankyrin-2, group VIA calcium-independent phospholipase A, group VIA cPLA2, group VIA phospholipase A2, Group VIA PLA2, Group VIA-3, group X secreted phospholipase A2, GS2, GV sPLA2, GVI PLA2, GVIA, GVIA iPLA2, GVIA PLA2, GVIA-iPLA2, GVIIA PLA2, GX, GX sPLA2, GXII sPLA2, GXIIA, GXIII sPLA2, hGX sPLA2, hIBPLA2, hIIAPLA2, human group IB PLA2, human group IIA phospholipase A2, IB PLA2, immune-associated phospholipase A2, imperatoxin, intercro, IPLA-1, iPLA2, iPLA2-gamma, jerdoxin, lecithinase A, lipoprotein associated phospholipase A2, lipoprotein-associated phospholipase A2, lipoprotein-associated PLA2, LLPL, LM-PLA2-I, LM-PLA2-II, LmTX-I, LmTX-II, Lp-PLA2, Lpla2, Lys49 phospholipase A2 homologue, Lys49-phospholipase A2 homologue, Lys49-PLA2, lysophospholipase, lysosomal phospholipase A2, lysosomal PLA2, mAoPlaA, marine snail digestive phospholipase A2, megacin A-216, MiDCA1, milleporin-1, MjTX-II, More, MP-III 4R, mSDPL, MsPLA2-I, Muscarinic inhibitor, mycotoxin II, myotoxic Asp49-phospholipase A2, myotoxic phospholipase A2, Myotoxin, myotoxin I, NAJPLA-2A, NAJPLA-2B, NAJPLA-2C, natratoxin, neuropathy target esterase, neutral anticoagulant secretory phospholipase A2, Nigexine, NK-PLA2-I, NK-PLA2-II, NN-X-PLA2, NN-XI-PLA2, NN-XIa-PLA2, NND-IV-PLA2, Non-pancreatic secretory phospholipase A2, Notechis 11'2, Notexin, notexinII-1, NPLA, NPS-PLA2, OHV A-PLA2, OHV-APLA2, OPLA2, PAF acetyl hydrolase/oxidized lipid LpPLA2, PAF-AH, pancreatic phospholipase A2, pancreatic-type PLA2, patatin, peroxiredoxin 6, pgPLA 1a/pgPLA 2a, PhlA, phosphatidase, phosphatide 2-acylhydrolase, phosphatidolipase, Phosphatidylcholine 2-acylhydrolase, Phosphatidylcholine 2-acylhydrolase GIIC, Phosphatidylcholine 2-acylhydrolase GIID, Phosphatidylcholine 2-acylhydrolase GIIE, Phosphatidylcholine 2-acylhydrolase GIIF, Phosphatidylcholine 2-acylhydrolase GIII, Phosphatidylcholine 2-acylhydrolase GX, Phosphatidylcholine 2-acylhydrolase GXII, Phosphatidylcholine 2-acylhydrolase GXIII, phospholipase A, phospholipase A2, phospholipase A2 D49, phospholipase A2 gamma, Phospholipase A2 inhibitor, phospholipase A2 neurotoxin, phospholipase A2 PA-11, phospholipase A2alpha, phospholipase A2IValpha, phospholipase A2s, phospholipin, pkP5, Pla, PLA1, PLA2, PLA2 CM2, PLA2 neurotoxin, PLA2-10, PLA2-1B, PLA2-2A, PLA2-2C, PLA2-H, PLA2-I, PLA2-L, PLA2-V, PLA2-VI, PLA2-VII, PLA2alpha, PLA2G1B, PLA2G2D, Pla2g4a, Pla2g4b, PLA2G4C, PLA2G4D, PLA2G6, PLA2IID, PLA2III, PLA2IValpha, PLA2s, platelet activating factor acetyl hydrolase, platelet activating factor acetyl hydrolase/oxidized lipid LpPLA2, platelet-activating factor acetylhydrolase, platlet-activating factor acetylhydrolase, plpA, ppPLA2, Prdx6, promutoxin, PrTX-1, PrTX-I, PrTX-III, Pt-PLA1, Pt-PLA2, R49 PLA2, RVV acidic PLA2-I, RVV-7, RVVA-PLA2-I, SCO1048 protein, Scol/Pla, secreted group IID phospholipase A2, secreted human phospholipase A2, secreted phospholipase A2, secreted phospholipaseA2 neurotoxin, secreted phospholipases A2, secreted PLA2, secreted sPLA2, secretory Ca2+-dependent PLA2, secretory phospholipase, secretory phospholipase A2, secretory phospholipase A2 group IIA, secretory phospholipase A2 type IB, secretory phospholipase A2 type IIA, secretory phospholipase A2-alpha, secretory PLA2s, Secretory-type PLA, stroma-associated homolog, snake presynoptic phospholipase A2 neurotoxin, SPAN, specific phospholipase A2, sPLA(2), sPLA(2)-IID, sPLA(2)-IIE, sPLA(2)-IIF, sPLA-V, sPLA2, sPLA2 GIB, sPLA2 GIIA, sPLA2 IB, sPLA2 type IIA, sPLA2(IIA), sPLA2-IB, sPLA2-IIA, sPLA2-IID, sPLA2-IIE, sPLA2-IIF, sPLA2-V, sPLA2-X, sPLA2:, sPLA2IB2, svPLA2, taipoxin, textilotoxin, thrombin inhibitor from Naja haje, TI-Nh, TMV-K49, toxin I, Toxin VI, Toxin VI:5, TPP, trimorphin, TTS-2.2, Tx-1, TX-I, type III cytotoxin, type VIIA PLA2, zhaoermiatoxin

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.4 phospholipase A2

Cloned

Cloned on EC 3.1.1.4 - phospholipase A2

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
basic-acidic hybrid PLA2 cDNA, cloned into the expression vector pBLMVL2 for later transformation in Escherichia coli
-
C-terminal His6-tagged GIVA PLA2 expressed using recombinant baculovirus in a suspension culture of Sf9 insect cells
-
cDNA cloned into the pGEM-T vector and transformed into Escherichia coli strain DH5alpha
cDNA coding for the predicted mature PLA2 protein, amino acids 1-128 without the signal peptide, designed with optimized codons for high level expression in Escherichia coli strain Tuner (DE3) LacI
CHO-K1 cells or HEK cells stably expressing N-terminal enhanced green fluorescent protein-cPLA2alpha fusion protein
-
chromosomal location at 1G1, phylogenetic tree
cPLA2-alpha and sPLA2s
cPLA2gamma, expressed in Sf9 cells, lung fibroblasts and KEK293 cells
-
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozymes sPLA2-X in Spodoptery frugiperda Sf9 cells using the baculovirus transfection system, expression of wild-type and D47K mutant isozymes sPLA2-X in HEK-293 cells
-
DNA-coding sequence subcloned into the vector pET3a, and expressed as inclusion bodies in Escherichia coli strain BL21(DE3)[pLysS]
-
expressed in Escherichia coli
expressed in Escherichia coli Top10 cells
expressed in insect cells (Sf9)
expressed in Nicotiana tabacum leaves
-
expressed in Sf9 insect cells
expression in COS-7 cell
expression in Escherichia coli
expression in HEK293 cells
expression in Pichia pastoris GS115 under the control of AOX1 promoter
expression in yeast
-
expression of AtsPLA2-alpha in Escherichia coli strain Bl21(DE3) in inclusion bodies
-
expression of full-length wild-type and mutant cPLA2alphas containing the affinity peptide YHHHHHH fused to the C-terminal Ala749, involving a recombinant adenovirus
-
expression of His-tagged wild-type and mutant enzymes
-
expression of isozyme hIBPLA2 and wild-type and mutant isozymes hIIAPLA2 in Escherichia coli strain BL21(DE3)
expression of isozyme sPLA2IB2 in HEK293 cells
expression of isozymes in HEK293 cells
expression of sPLA2s from groups IIE, III, V, and X in HEK-293T and CHO-K1 cells and secretion of the recombinant protein. Enhancement of expression and secretion of the isozymes by co-expression of several different engineered His6-tagged human small ubiquitin-like modifier, i.e. SUMO, proteins, overview
expression of untagged enzyme
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies
-
for construction of the cPLA2-luc plasmid, cPLA2 promoter, a region spanning -2,375 to -75 bp, cloned into pGL3-basic vector
-
from cDNA library prepared from mRNA of the venomous glands
-
full length human cPLA2, residues 1-749, expressed in E5-CHO-cells
-
gene lpla2, overexpression in MDKC cells
-
GIVA PLA2 and GVIIA PLA2
-
group-IB sPLA2 expressed from Escherichia coli
GVIA-2 iPLA2 is membrane-associated when overexpressed in COS-7 cells as well as rat vascular smooth muscle cells. The human group VIA PLA2 gene yields multiple splice variants, including GVIA-1, GVIA-2, GVIA-3 PLA2, GVIA ankyrin-1 and GVIA ankyrin-2
-
into baculovirus, transfection of SF9 insect cells
-
into M13-vector mp8 for site directed mutagenesis, into pUEX 1 vector for tranformation of Escherichia coli
-
into pAcYM1, transfer into baculovirus, transfection of Sf9 S.frugiperda cells
-
into pBR322 and pCI1857, transformation of Escherichia coli strains HB101 and AB1157
into pcDNA3.1 for later transfections
into pET21a vector for transformation of Escherichia coli strain BL21(DE3) and into pFasBac baculovirus-vector for transfection of Sf9 S. frugiperda cells
-
into pET21a vector, transformation of Escherichia coli strain BL21(DE3)
-
into pET28a(+) vector, transformation of Escherichia coli strain BL21
-
into pQE-30 of Escherichia coli
-
isoform G-3 PLA2
-
isozyme IPLA-1, expression of GST-fusion enzyme in Escherichia coli strain Rosetta2 (DE3)
-
isozymes BinTx-I and BinTx-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis, sequence comparisons, overview
isozymes DrK-aI, DrK-aII, DrK-bI, and DrK-bII, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
isozymes DsM-bI and DsM-S1, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
ligated into PCR-Blunt vector and transformed into Escherichia coli DH5alpha cells
-
Lp-PLA2 gene, cloning from differentiated THP-1 cells, DNA and amino acid sequence determination and anaylsis, expression of C-terminally His6-tagged enzyme in Pichia pastoris strain GS115 and Escherichia coli
macrophages transfected with the construct EGFP-cPLA2alpha, ECFP-S505A or ECFP-S505E
-
overexpression in Escherichia coli BL21 (DE3)
overexpression of His-tagged and/or fluoresence-tagged phospholipase A2 in COS-7 cells, perinuclear region, membranes and cytosol, or 3T3-L1 preadipocytic cells
-
overexpression of type IIa human PLA2 in mice correlating with elevated atherosclerotic lesions, membranes with elevated cholesterol content can be generated by PLA2 activation, mechanism, overview
-
PCR product recombined into the pET-28a expression vector with the coding region in frame with the 6 × His tag, expressed in Escherichia coli BL-21 (DE3)
Phenotypes (megacin production and effect of UV irradiation) of the primary clone carrying the recombinant plasmid pMGC1 are similar to those observed with the parental Bacillus megaterium 216 strain. The Bacillus megaterium clone (pMGC3) carrying the 5494-bp HindIII subfragment of the original 10 kb insert exhibits a wider inhibitory zone around noninduced colonies.
PLA2 injected (subcutaneous) into the right hind paw of mice
-
secreted phospholipase A2, DNA and amino acid sequence determination and analysis
secreted phospholipase A2, DNA and amino acid sequence determination and analysis, expression of in Escherichia coli strain BL21(DE3) in inclusion bodies
-
wild type and mutant cPLA2s overexpression in HEK293 EcR or HEK293-BLT1 cells, expression of fluorescence of FLAG-tagged cPLA2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method
-