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Literature summary for 3.1.1.4 extracted from
- Functional and structural characterization of a thermostable phospholipase A2 from a Sparidae Fish (Diplodus annularis) (2017), J. Agric. Food Chem., 65, 2468-2480 .
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the enzyme is active and stable at higher temperatures, which suggests its great potential in biotechnological applications | Diplodus annularis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21 (DE3) | Diplodus annularis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Diplodus annularis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | maximal activity in the presence of 6 mM Ca2+ | Diplodus annularis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
enzyme expressed in Escherichia coli and refolded, MALDI-TOF spectrometry | Diplodus annularis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diplodus annularis | A0A1L4FQZ9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Diplodus annularis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O | - |
Diplodus annularis | 1-palmitoyl-sn-glycero-3-phosphocholine + oleate | - |
? | |
| additional information | the enzyme is able to hydrolyze mainly the sn-2 position of phospholipids | Diplodus annularis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PLA2 | - |
Diplodus annularis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Diplodus annularis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 40% of maximal activity, 60°C: about 35% of maximal activity | Diplodus annularis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
15 min, no loss of activity | Diplodus annularis |
| 60 | - |
15 min, 20% loss of activity | Diplodus annularis |
| 70 | - |
15 min, 80% loss of activity | Diplodus annularis |
| 80 | - |
15 min, 95% loss of activity | Diplodus annularis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Diplodus annularis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6.0: about 75% of maximal activity, pH 10.0: about 80% of maximal activity | Diplodus annularis |
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