Literature summary for 3.1.1.4 extracted from

Verlotta, A.; Trono, D.
Expression, purification and refolding of active durum wheat (Triticum durum Desf.) secretory phospholipase A2 from inclusion bodies of Escherichia coli (2014), Protein Expr. Purif., 101, 28-36.

Search for more literature for 3.1.1.4

Application

Application	Comment	Organism
synthesis	protocol for the overexpression ofisoform PLA2III in Escherichia coli in the form of inclusion bodies, and for its purification and refolding using the Gateway system (Invitrogen) for the expression of a large quantity of the mature with an N-terminal His-tag, and Ni-affinity chromatography for purification. The purified recombinant PLA2III fusion protein is then refolded using a step-wise dialysis approach. About 40 mg purified and active protein is obtained from 1 l of cell culture	Triticum turgidum subsp. durum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Triticum turgidum subsp. durum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Triticum turgidum subsp. durum	M1GL46	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine + H2O	-	Triticum turgidum subsp. durum	1-palmitoyl-sn-glycero-3-phosphocholine + linoleic acid	-	?

Synonyms

Synonyms	Comment	Organism
PLA2III	-	Triticum turgidum subsp. durum
secretory phospholipase A2	-	Triticum turgidum subsp. durum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)