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Literature summary for 3.1.1.4 extracted from

  • Pan, F.M.; Chao, S.C.; Wu, S.H.; Chang, W.C.; Chiou, S.H.
    Characterization of phospholipase A2 (PLA2) from Taiwan Cobra: Isoenzymes and their site-directed mutants (1998), Biochem. Biophys. Res. Commun., 250, 154-160.
    View publication on PubMed
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
into pQE-30 of Escherichia coli Naja atra

Protein Variants

Protein Variants Comment Organism
D97N reduced activity of acidic secretory isoforms PLA2-1 and PLA2-2 Naja atra
H47A no activity of acidic secretory isoforms PLA2-1 and PLA2-2 Naja atra
H47R no activity of acidic secretory isoforms PLA2-1 and PLA2-2 Naja atra

Localization

Localization Comment Organism GeneOntology No. Textmining
secretory granule
-
 Naja atra 30141
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13220
-
 PLA2-2, SDS-PAGE and mass spectrometry Naja atra
13260
-
 PLA2-1, SDS-PAGE and mass spectrometry Naja atra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Naja atra myotoxicity ?
-
 ?
phospholipids + H2O Naja atra presynaptic neurotoxicity of neutral PLA2 ?
-
 ?
phospholipids + H2O Naja atra cardiac toxicity ?
-
 ?
phospholipids + H2O Naja atra hemolytic activity of basic PLA2 ?
-
 ?
phospholipids + H2O Naja atra influence on platelet aggregation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Naja atra
-
 Taiwan cobra
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity chromatography Naja atra

Renatured (Commentary)

Renatured (Comment) Organism
-
 Naja atra

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Naja atra
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Naja atra 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
phospholipids + H2O myotoxicity Naja atra ?
-
 ?
phospholipids + H2O presynaptic neurotoxicity of neutral PLA2 Naja atra ?
-
 ?
phospholipids + H2O cardiac toxicity Naja atra ?
-
 ?
phospholipids + H2O hemolytic activity of basic PLA2 Naja atra ?
-
 ?
phospholipids + H2O influence on platelet aggregation Naja atra ?
-
 ?