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Literature summary for 3.1.1.4 extracted from

  • Tucker, D.E.; Ghosh, M.; Ghomashchi, F.; Loper, R.; Suram, S.; St John, B.; Girotti, M.; Bollinger, J.G.; Gelb, M.H.; Leslie, C.C.
    Role of phosphorylation and basic residues in the catalytic domain of cytosolic phospholipase A2alpha in regulating interfacial kinetics and binding and cellular function (2009), J. Biol. Chem., 284, 9596-9611.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length wild-type and mutant cPLA2alphas containing the affinity peptide YHHHHHH fused to the C-terminal Ala749, involving a recombinant adenovirus Homo sapiens

Protein Variants

Protein Variants Comment Organism
A206K site-directed mutagenesis Homo sapiens
D43N site-directed mutagenesis Homo sapiens
K488N/K543N/K544N site-directed mutagenesis, the mutant shows 2fold increased activity compared to the wild-type enzyme Homo sapiens
additional information reconstitution of immortalized mouse lung fibroblasts lacking endogenous cPLA2alpha, IMLF-/-, with human wild-type and cPLA2alpha mutants, overview Homo sapiens
S505A site-directed mutagenesis Homo sapiens
S505A/S727A site-directed mutagenesis Homo sapiens
S505A/S727A/S515A site-directed mutagenesis Homo sapiens
S515A site-directed mutagenesis Homo sapiens
S727A site-directed mutagenesis Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane calcium, phosphorylation at Ser505 and the putative phosphatidylinositol 4,5-bisphosphate binding site regulate translocation and arachidonic acid release, overview Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ calcium, phosphorylation at Ser505 and the putative phosphatidylinositol 4,5-bisphosphate binding site regulate translocation and arachidonic acid release, overview. Influx of extracellular calcium is essential for stable binding of cPLA2alpha to Golgi and arachidonic acid release. phosphatidylinositol 4,5-bisphosphate improves Ca2+ binding by about 2fold Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens cPLA2alpha is regulated by phosphorylation at Ser505 and calcium-induced translocation to membranes ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein calcium, phosphorylation at Ser505 and the putative phosphatidylinositol 4,5-bisphosphate binding site regulate translocation and arachidonic acid release, overview. Preparation of phosphorylated cPLA2alpha forms: cPLA2alpha-505P and cPLA2alpha-515P Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant full-length wild-type and mutant cPLA2alphas containing the affinity peptide YHHHHHH Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) + H2O
-
Homo sapiens 1-palmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) + palmitic acid
-
?
1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphocholine + H2O
-
Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonic acid
-
?
1-stearoyl-2-arachidonyl-phosphatidylinositol 4,5-bisphosphate + H2O
-
Homo sapiens 1-stearoyl-sn-glycero-3-phosphoinositol 4,5-bisphosphate + arachidonic acid
-
?
additional information cPLA2alpha is regulated by phosphorylation at Ser505 and calcium-induced translocation to membranes Homo sapiens ?
-
?
additional information interfacial binding and kinetics of cPLA2 and its phosphorylated forms in vitro, lipid vesicle formation, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
cPLA2alpha
-
Homo sapiens
group IVA cytosolic phospholipase A2
-
Homo sapiens
phospholipase A2alpha
-
Homo sapiens
PLA2alpha
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Homo sapiens