Cloned (Comment) | Organism |
---|---|
expression of full-length wild-type and mutant cPLA2alphas containing the affinity peptide YHHHHHH fused to the C-terminal Ala749, involving a recombinant adenovirus | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A206K | site-directed mutagenesis | Homo sapiens |
D43N | site-directed mutagenesis | Homo sapiens |
K488N/K543N/K544N | site-directed mutagenesis, the mutant shows 2fold increased activity compared to the wild-type enzyme | Homo sapiens |
additional information | reconstitution of immortalized mouse lung fibroblasts lacking endogenous cPLA2alpha, IMLF-/-, with human wild-type and cPLA2alpha mutants, overview | Homo sapiens |
S505A | site-directed mutagenesis | Homo sapiens |
S505A/S727A | site-directed mutagenesis | Homo sapiens |
S505A/S727A/S515A | site-directed mutagenesis | Homo sapiens |
S515A | site-directed mutagenesis | Homo sapiens |
S727A | site-directed mutagenesis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | calcium, phosphorylation at Ser505 and the putative phosphatidylinositol 4,5-bisphosphate binding site regulate translocation and arachidonic acid release, overview | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | calcium, phosphorylation at Ser505 and the putative phosphatidylinositol 4,5-bisphosphate binding site regulate translocation and arachidonic acid release, overview. Influx of extracellular calcium is essential for stable binding of cPLA2alpha to Golgi and arachidonic acid release. phosphatidylinositol 4,5-bisphosphate improves Ca2+ binding by about 2fold | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | cPLA2alpha is regulated by phosphorylation at Ser505 and calcium-induced translocation to membranes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | calcium, phosphorylation at Ser505 and the putative phosphatidylinositol 4,5-bisphosphate binding site regulate translocation and arachidonic acid release, overview. Preparation of phosphorylated cPLA2alpha forms: cPLA2alpha-505P and cPLA2alpha-515P | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant full-length wild-type and mutant cPLA2alphas containing the affinity peptide YHHHHHH | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood platelet | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) + H2O | - |
Homo sapiens | 1-palmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) + palmitic acid | - |
? | |
1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphocholine + H2O | - |
Homo sapiens | 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonic acid | - |
? | |
1-stearoyl-2-arachidonyl-phosphatidylinositol 4,5-bisphosphate + H2O | - |
Homo sapiens | 1-stearoyl-sn-glycero-3-phosphoinositol 4,5-bisphosphate + arachidonic acid | - |
? | |
additional information | cPLA2alpha is regulated by phosphorylation at Ser505 and calcium-induced translocation to membranes | Homo sapiens | ? | - |
? | |
additional information | interfacial binding and kinetics of cPLA2 and its phosphorylated forms in vitro, lipid vesicle formation, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cPLA2alpha | - |
Homo sapiens |
group IVA cytosolic phospholipase A2 | - |
Homo sapiens |
phospholipase A2alpha | - |
Homo sapiens |
PLA2alpha | - |
Homo sapiens |