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Literature summary for 3.1.1.4 extracted from
- Cloning and characterization of the DNA region responsible for Megacin A-216 production in Bacillus megaterium 216 (2008), J. Bacteriol., 190, 6448-6457.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Phenotypes (megacin production and effect of UV irradiation) of the primary clone carrying the recombinant plasmid pMGC1 are similar to those observed with the parental Bacillus megaterium 216 strain. The Bacillus megaterium clone (pMGC3) carrying the 5494-bp HindIII subfragment of the original 10 kb insert exhibits a wider inhibitory zone around noninduced colonies. | Priestia megaterium |
General Stability
| General Stability | Organism |
|---|---|
| stored at -80°C for several months with only slight loss of biological activity | Priestia megaterium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration, native molecular mass is consistent with an alpha2beta2, alpha beta gamma or gamma2 composition. The largest protein (gamma chain) is the full-length product of ORF293A, whereas the shorter alpha and beta chains appear to be products of proteolytic cleavage between Gln-185 and Val-186 of the gamma chain. | Priestia megaterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Priestia megaterium | - |
additional information | Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills Bacillus megaterium and a few other bacterial species. | ? |  |
| additional information | Priestia megaterium 216 | - |
additional information | Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills Bacillus megaterium and a few other bacterial species. | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | A7LGL2 | gene meg A | - |
| Priestia megaterium 216 | A7LGL2 | gene meg A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| all steps are performed at 4°C, precipitation, gel filtration | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Priestia megaterium | additional information | Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills Bacillus megaterium and a few other bacterial species. | ? | |
| additional information | - |
Priestia megaterium 216 | additional information | Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills Bacillus megaterium and a few other bacterial species. | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | Purified biologically active megacin A-216 preparations contain three proteins. The largest protein is the full-length translation product of the megA gene, whereas the two shorter proteins are fragments of the long protein created by cleavage between Gln-185 and Val-186. | Priestia megaterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| megacin A-216 | - |
Priestia megaterium |
| phospholipase A2 | - |
Priestia megaterium |
| PLA2 | - |
Priestia megaterium |
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