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3.1.1.4: phospholipase A2

This is an abbreviated version!
For detailed information about phospholipase A2, go to the full flat file.

Word Map on EC 3.1.1.4

Reaction

phosphatidylcholine
+
H2O
=
1-acylglycerophosphocholine
 +
a carboxylate

Synonyms

1-CysPrx, 1-cysteine peroxiredoxin, 14 kDa phospholipase A2, A2-IIA, Acanmyotoxin-1, acidic Asp49 phospholipase A2, acidic Ca2+-independent phospholipase A2, acidic phospholipase A2, ACS, adiponutrin, AdPLA, Agkistrotoxin, AgkTx-II, aiPLA2, alpha-type phospholipase A2, amdI1, Ammodytin I2, ammodytoxin A, APLA, APP-D-49, Arg49 phospholipase A2, Arg49 phospholipase A2 homologue, Asp49 basic myotoxic PLA2, Asp49 PLA2, Asp49-PLA2, ASPLA1, ASPLA10, ASPLA11, ASPLA12, ASPLA13, ASPLA14, ASPLA15, ASPLA16, ASPLA17, ASPLA2, ASPLA3, ASPLA4, ASPLA5, ASPLA6, ASPLA7, ASPLA8, ASPLA9, Ats-PLA2-alpha, AtsPLA2-alpha, AtsPLA2-gamma, ATX, BaltPLA2, basic Asp49 PLA2, Basic protein I/II, BaTX, BinTx-I, BinTx-II, BJ-PLA2, Bj-V, BJUPLA2, Bothropstoxin-I, BPI/BPII, BthTX, BthTX-1, BthTX-I, BthTX-II, bvPLA2, Ca+2-independent PLA2, Ca2+ independent PLA2, Ca2+-independent iPLA2, Ca2+-independent phospholipase A2, Ca2+-independent PLA2, CaI-PLA2, calcium-d-independent cytosolic PLA2, calcium-dependent cytosolic PLA2, calcium-independent group VIA iPLA2, calcium-independent phospholipase A2, Caudoxin, Cdt PLA2, class XIB phospholipase A2, ColTx-I, cPLA2, cPLA2 alpha, cPLA2-alpha, cPLA2-gamma, cPLA2-zeta2, cPLA2alpha, cPLA2beta, cPLA2delta, cPLA2epsilon, cPLA2zeta, cPm09, Cr-IV 1, Cro, crotapotin, crotoxin, CTX, cytoplasmic phospholipase A2, cytosolic calcium-dependent PLA2, cytosolic cPLA2, cytosolic gIVaPLA2, cytosolic group IVA cPLA2, cytosolic group IVa phospholipase A2, cytosolic phospholipase A2, cytosolic phospholipase A2 alpha, cytosolic phospholipase A2-alpha, cytosolic phospholipase A2alpha, cytosolic PLA2, cytotoxin ExoU, Daboxin P, DrK-aI, DrK-aII, DrK-bI, DrK-bII, DrPLA2, DsM-bI, DsM-S1, Enhancing factor, exoenzyme U, ExoU, ExoU-specific PLA2, GIA cobra venom PLA2, GIIA, GIIA PLA2, GIIA sPLA2, GIIC sPLA2, GIID, GIID sPLA2, GIIE, GIIE sPLA2, GIIF, GIIF sPLA2, GIII sPLA2, GIIIsPLA2, GIVA, GIVA cPLA2, GIVA phospholipase A2, GIVA PLA2, GIVD cPLA2delta, GIVF, Gln49-PLA2, group IA PLA2, Group IB phospholipase A2, group IB PLA2, group II snake venom phospholipase A2, Group IIA phospholipase A2, group IIA PLA2, group IIA secretory phospholipase A2, group IIA secretory PLA2, group IID secretory phospholipase A2, group III Glu62-phospholipase A2, group III PLA2, group III sPLA2, group IV cPLA2, group IVA cPLA2, group IVA cytosolic phospholipase A2, group IVA phospholipase A2, group IVA PLA2, group IVB phospholipase A2, Group V phospholipase A2, group V secreted phospholipase A2, Group VI phospholipase A2, Group VIA Ankyrin-1, Group VIA Ankyrin-2, group VIA calcium-independent phospholipase A, group VIA cPLA2, group VIA phospholipase A2, Group VIA PLA2, Group VIA-3, group X secreted phospholipase A2, GS2, GV sPLA2, GVI PLA2, GVIA, GVIA iPLA2, GVIA PLA2, GVIA-iPLA2, GVIIA PLA2, GX, GX sPLA2, GXII sPLA2, GXIIA, GXIII sPLA2, hGX sPLA2, hIBPLA2, hIIAPLA2, human group IB PLA2, human group IIA phospholipase A2, IB PLA2, immune-associated phospholipase A2, imperatoxin, intercro, IPLA-1, iPLA2, iPLA2-gamma, jerdoxin, lecithinase A, lipoprotein associated phospholipase A2, lipoprotein-associated phospholipase A2, lipoprotein-associated PLA2, LLPL, LM-PLA2-I, LM-PLA2-II, LmTX-I, LmTX-II, Lp-PLA2, Lpla2, Lys49 phospholipase A2 homologue, Lys49-phospholipase A2 homologue, Lys49-PLA2, lysophospholipase, lysosomal phospholipase A2, lysosomal PLA2, mAoPlaA, marine snail digestive phospholipase A2, megacin A-216, MiDCA1, milleporin-1, MjTX-II, More, MP-III 4R, mSDPL, MsPLA2-I, Muscarinic inhibitor, mycotoxin II, myotoxic Asp49-phospholipase A2, myotoxic phospholipase A2, Myotoxin, myotoxin I, NAJPLA-2A, NAJPLA-2B, NAJPLA-2C, natratoxin, neuropathy target esterase, neutral anticoagulant secretory phospholipase A2, Nigexine, NK-PLA2-I, NK-PLA2-II, NN-X-PLA2, NN-XI-PLA2, NN-XIa-PLA2, NND-IV-PLA2, Non-pancreatic secretory phospholipase A2, Notechis 11'2, Notexin, notexinII-1, NPLA, NPS-PLA2, OHV A-PLA2, OHV-APLA2, OPLA2, PAF acetyl hydrolase/oxidized lipid LpPLA2, PAF-AH, pancreatic phospholipase A2, pancreatic-type PLA2, patatin, peroxiredoxin 6, pgPLA 1a/pgPLA 2a, PhlA, phosphatidase, phosphatide 2-acylhydrolase, phosphatidolipase, Phosphatidylcholine 2-acylhydrolase, Phosphatidylcholine 2-acylhydrolase GIIC, Phosphatidylcholine 2-acylhydrolase GIID, Phosphatidylcholine 2-acylhydrolase GIIE, Phosphatidylcholine 2-acylhydrolase GIIF, Phosphatidylcholine 2-acylhydrolase GIII, Phosphatidylcholine 2-acylhydrolase GX, Phosphatidylcholine 2-acylhydrolase GXII, Phosphatidylcholine 2-acylhydrolase GXIII, phospholipase A, phospholipase A2, phospholipase A2 D49, phospholipase A2 gamma, Phospholipase A2 inhibitor, phospholipase A2 neurotoxin, phospholipase A2 PA-11, phospholipase A2alpha, phospholipase A2IValpha, phospholipase A2s, phospholipin, pkP5, Pla, PLA1, PLA2, PLA2 CM2, PLA2 neurotoxin, PLA2-10, PLA2-1B, PLA2-2A, PLA2-2C, PLA2-H, PLA2-I, PLA2-L, PLA2-V, PLA2-VI, PLA2-VII, PLA2alpha, PLA2G1B, PLA2G2D, Pla2g4a, Pla2g4b, PLA2G4C, PLA2G4D, PLA2G6, PLA2IID, PLA2III, PLA2IValpha, PLA2s, platelet activating factor acetyl hydrolase, platelet activating factor acetyl hydrolase/oxidized lipid LpPLA2, platelet-activating factor acetylhydrolase, platlet-activating factor acetylhydrolase, plpA, ppPLA2, Prdx6, promutoxin, PrTX-1, PrTX-I, PrTX-III, Pt-PLA1, Pt-PLA2, R49 PLA2, RVV acidic PLA2-I, RVV-7, RVVA-PLA2-I, SCO1048 protein, Scol/Pla, secreted group IID phospholipase A2, secreted human phospholipase A2, secreted phospholipase A2, secreted phospholipaseA2 neurotoxin, secreted phospholipases A2, secreted PLA2, secreted sPLA2, secretory Ca2+-dependent PLA2, secretory phospholipase, secretory phospholipase A2, secretory phospholipase A2 group IIA, secretory phospholipase A2 type IB, secretory phospholipase A2 type IIA, secretory phospholipase A2-alpha, secretory PLA2s, Secretory-type PLA, stroma-associated homolog, snake presynoptic phospholipase A2 neurotoxin, SPAN, specific phospholipase A2, sPLA(2), sPLA(2)-IID, sPLA(2)-IIE, sPLA(2)-IIF, sPLA-V, sPLA2, sPLA2 GIB, sPLA2 GIIA, sPLA2 IB, sPLA2 type IIA, sPLA2(IIA), sPLA2-IB, sPLA2-IIA, sPLA2-IID, sPLA2-IIE, sPLA2-IIF, sPLA2-V, sPLA2-X, sPLA2:, sPLA2IB2, svPLA2, taipoxin, textilotoxin, thrombin inhibitor from Naja haje, TI-Nh, TMV-K49, toxin I, Toxin VI, Toxin VI:5, TPP, trimorphin, TTS-2.2, Tx-1, TX-I, type III cytotoxin, type VIIA PLA2, zhaoermiatoxin

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.4 phospholipase A2

Molecular Weight

Molecular Weight on EC 3.1.1.4 - phospholipase A2

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
-
x * 10000, SDS-PAGE
100000
103000
-
centrifugation in sucrose density gradients
105000
11000
-
recombinant secreted phospholipase A2, gel filtration
110000
-
gel filtration
114000
-
isoform cPLA2-alpha
12000
-
gel filtration
12809
-
mass spectrometry
13000
13100
-
PAGE
13198
2 * 13198, MALDI-TOF
13220
-
PLA2-2, SDS-PAGE and mass spectrometry
13260
-
PLA2-1, SDS-PAGE and mass spectrometry
13262
-
x * 13262, MALDI-MS
13346
-
1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS
13500
-
gel filtration
135000
-
transfected protein
13530
-
1 * 13000, recombinant secreted phospholipase A2, SDS-PAGE, 1 * 13530, secreted phospholipase A2, MALDI-TOF mass spectrometry
13597
electrospray ionization mass spectrometry
13610
2 * 13610, claculated, asymmetric homodimer, crystallization data
13627
x * 13627, mass spectrometry. x * 13638, calculated from sequence
13638
x * 13627, mass spectrometry. x * 13638, calculated from sequence
13649
-
x * 19000, SDS-PAGE, x * 13649, calculated
13700
-
natural PLA2, SDS-PAGE
13723
13749
-
1 * 13749
13750
13788
x * 13975, isozyme BinTx-I, sequence calculation, x * 13788, isozyme BinTx-II, sequence calculation
13793
-
1 * 14221, isoform Tpu-K49a, 1 * 14113, isoform Tpu-K49b, 1 * 13913, isoform Tpu-G6D49, 1 * 13723, isoform Tpu-E6a, 1 * 13979, isoform Tpu-E6b, 1 * 13793, isoform Tpu-E6c, ESI-MS
13800
-
-
13810
-
mass spectrometry
13840
-
MALDI-TOF mass spectrometry
13862
-
x * 13862, MALDI-TOF mass spectrometry
13871
-
x * 13871, mass spectrometry, x * 14000, SDS-PAGE
13880
-
MiPLA-3 isoform, mass spectrometry
13881
-
x * 13881, mass spectrometry, x * 17000, SDS-PAGE
13900
13913
-
1 * 14221, isoform Tpu-K49a, 1 * 14113, isoform Tpu-K49b, 1 * 13913, isoform Tpu-G6D49, 1 * 13723, isoform Tpu-E6a, 1 * 13979, isoform Tpu-E6b, 1 * 13793, isoform Tpu-E6c, ESI-MS
13950
13960
-
1 * 14034, isoform Tbo-K49, 1 * 13960, isoform Tbo-G6D49, 1 * 13723, isoform Tpu-E6, ESI-MS
13972
13975
x * 13975, isozyme BinTx-I, sequence calculation, x * 13788, isozyme BinTx-II, sequence calculation
13979
-
1 * 14221, isoform Tpu-K49a, 1 * 14113, isoform Tpu-K49b, 1 * 13913, isoform Tpu-G6D49, 1 * 13723, isoform Tpu-E6a, 1 * 13979, isoform Tpu-E6b, 1 * 13793, isoform Tpu-E6c, ESI-MS
13984
-
x * 13984, mass spectrometry, x * 14000, SDS-PAGE
13996
-
x * 14000, SDS-PAGE, x * 13996, mass spectrometry
140000
-
gel filtration, multimeric complex
14020
-
MiPLA-4 isoform, mass spectrometry
14034
-
1 * 14034, isoform Tbo-K49, 1 * 13960, isoform Tbo-G6D49, 1 * 13723, isoform Tpu-E6, ESI-MS
14075
-
MALDI TOF/TOF
14100
14113
-
1 * 14221, isoform Tpu-K49a, 1 * 14113, isoform Tpu-K49b, 1 * 13913, isoform Tpu-G6D49, 1 * 13723, isoform Tpu-E6a, 1 * 13979, isoform Tpu-E6b, 1 * 13793, isoform Tpu-E6c, ESI-MS
14145
calculated from sequence
14169
x * 16000, SDS-PAGE, x * 16310, calculated for immature enzyme, x * 14169, calculated for mature form
14170
-
calculated from sequence
14180
sequence analysis
14210
electrospray ionization
14221
-
1 * 14221, isoform Tpu-K49a, 1 * 14113, isoform Tpu-K49b, 1 * 13913, isoform Tpu-G6D49, 1 * 13723, isoform Tpu-E6a, 1 * 13979, isoform Tpu-E6b, 1 * 13793, isoform Tpu-E6c, ESI-MS
14249
-
x * 14249, MALDI-TOF mass spectrometry
14340
-
mass spectrometry
14350
-
1 * 14350, phospholipase A2 subunit, + 1 * 9490, crotapin subunit
15552
Micrurus dumerilii carinicauda
-
x * 15000, SDS-PAGE, x * 15552, calculated
15700
-
SDS-PAGE
15840
-
ethylpyridylation after total reduction of the enzyme, mass spectrometry
16000
16310
x * 16000, SDS-PAGE, x * 16310, calculated for immature enzyme, x * 14169, calculated for mature form
16500
-
1 * 16500 + 1 * 18000, SDS-PAGE
17000
17500
calculated from amino acid sequence
18000
180000
-
sedimentation velocity centrifugation
18500
-
SDS-PAGE
19000
-
x * 19000, SDS-PAGE, x * 13649, calculated
190000
-
and 350000, gel filtration
197000
-
gel filtration
200000
-
SDS-PAGE
200000 - 500000
-
isoform PLA2-H
21000
-
SDS-PAGE
25000
-
x * 25000
26000
-
-
26280
MALDI-TOF, dimer
28000
28500
-
2 * 28500, SDS-PAGE
28900
-
2 * 28900, calculated from amino acid sequence
29000
30000
32500
-
x * 32500, SDS-PAGE
34000
34500
-
dimer with a 16500 and a 18000 subunit, SDS-PAGE
350000
-
and 190000, gel filtration
39000
-
-
40000
42000
-
gel filtration
43000
44000
-
44000 Da and 43000 Da
44160
-
SDS-PAGE and liquid chromatography
45000
-
Western blot analysis
48000
-
SDS-PAGE
53000
-
SDS-PAGE
56000
-
SDS-PAGE
61000
63000
-
63000 Da and 77000 Da, iPLA2-gamma, SDS-PAGE
6500
-
gel filtration, underestimation seems obvious to authors
6500 - 7000
-
RVV-7, native PAGE
66000
gel filtration, native molecular mass is consistent with an alpha2beta2, alpha beta gamma or gamma2 composition. The largest protein (gamma chain) is the full-length product of ORF293A, whereas the shorter alpha and beta chains appear to be products of proteolytic cleavage between Gln-185 and Val-186 of the gamma chain.
7201 - 7224
-
RVV-7, mass spectrometry
75000
77000
-
63000 Da and 77000 Da, iPLA2-gamma, SDS-PAGE
78000
-
native PAGE
80000
85000
85000 - 88000
87000
88000
90000
9490
-
1 * 14350, phospholipase A2 subunit, + 1 * 9490, crotapin subunit
additional information
-
native isozyme IPLA-1 exhibits a Stokes radius of 6.2 nm similar to recombinant isozyme IPLA-1. Presence of ATP increases the experimental masses of the enzyme complexes to 430 and 250 kDa, which correspond to homopentameric and homotrimeric complexes, respectively