Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.4 extracted from

  • Franken, P.A.; van den Berg, L.; Huang, J.; Gunyuzlu, P.; Lugtigheid, R.B.; Verheij, H.M.; de Haas, G.H.
    Purification and characterization of a mutant human platelet phospholipase A2 expressed in Escherichia coli (1992), Eur. J. Biochem., 203, 89-98.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into M13-vector mp8 for site directed mutagenesis, into pUEX 1 vector for tranformation of Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
M20L according the gapped-duplex method, using amber selection Homo sapiens
M8L according the gapped-duplex method, using amber selection Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-tetradecanoylaminohexanol-1-phosphocholine competitive inhibition, R-enantiomer is a more potent inhibitor Homo sapiens
2-tetradecanoylaminohexanol-1-phosphoethanolamine competitive inhibition, R-enantiomer is a more potent inhibitor Homo sapiens
2-tetradecanoylaminohexanol-1-phosphoglycol competitive inhibition, R-enantiomer is a more potent inhibitor Homo sapiens
EDTA
-
Homo sapiens
EDTA
-
Sus scrofa
p-bromophenacyl bromide
-
Homo sapiens
p-bromophenacyl bromide
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
1,2-dioctanoyl-sn-glycero-3-phosphoglycol
-
Homo sapiens
0.1
-
1,2-dioctanoyl-sn-glycero-3-phosphoglycol
-
Sus scrofa
0.2
-
1,2-dioctanoyl-sn-glycero-3-phosphocholine
-
Homo sapiens
0.9
-
1,2-dinonanoyl-sn-glycero-3-phosphocholine
-
Homo sapiens
1.4
-
1,2-dihexanoyl-sn-glycero-3-phosphocholine
-
Sus scrofa
2.4
-
1,2-dihexanoyl-sn-glycero-3-phosphocholine [M8L and M20L]-loop-deleted PLA2 Homo sapiens
2.8
-
1,2-dihexanoyl-sn-glycero-3-phosphocholine loop deleted PLA2 Homo sapiens
3
-
1,2-diheptanoyl-sn-glycero-3-phosphocholine
-
Sus scrofa
3.2
-
1,2-dioctanoyl-sn-glycero-3-phosphocholine
-
Sus scrofa
3.6
-
1,2-diheptanoyl-sn-glycero-3-phosphocholine
-
Homo sapiens
4.3
-
1,2-dihexanoyl-sn-glycero-3-phosphocholine
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Ca2+
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
-
Sus scrofa
14000
-
heparin-sepharose affinity chromatography, SDS-PAGE after digestion with thrombin, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Homo sapiens influence on platelet aggregation ?
-
?
phospholipids + H2O Homo sapiens inflammatory diseases ?
-
?
phospholipids + H2O Homo sapiens release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins ?
-
?
phospholipids + H2O Sus scrofa release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Naja melanoleuca
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange chromatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Homo sapiens
-
pancreas
-
Sus scrofa
-
platelet
-
Homo sapiens
-
venom
-
Naja melanoleuca
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
substrate: dioleolyl-sn-phosphatidylcholine Homo sapiens
29
-
substrate: 1,2-dipalmitoyl-phosphatidylcholine Sus scrofa
30
-
substrate: 1,2-dipalmitoyl-phosphatidylcholine Homo sapiens
57
-
substrate: dimyristoyl-sn-phosphatidylcholine Homo sapiens
68
-
substrate: dimyristoyl-sn-phosphatidylcholine Sus scrofa
80
-
substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine Sus scrofa
81
-
substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine Sus scrofa
118
-
substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine Homo sapiens
124
-
substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine Homo sapiens
125
-
substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine Sus scrofa
155
-
substrate: dioleolyl-sn-phosphatidylethanolamine Homo sapiens
162
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine Sus scrofa
171
-
substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine Homo sapiens
172
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine Homo sapiens
208
-
substrate: dioleolyl phosphatidylglycerol Homo sapiens
210
-
substrate: dioleolyl-sn-phosphatidylethanolamine Naja melanoleuca
295
-
substrate: dioleolyl-sn-phosphatidylethanolamine Sus scrofa
320
-
substrate: dioleolyl-sn-phosphatidylcholine Sus scrofa
410
-
substrate: dioleolyl-sn-phosphatidylcholine Naja melanoleuca
530
-
substrate: dioleolyl phosphatidylglycerol Naja melanoleuca
615
-
substrate: dioleolyl phosphatidylglycerol Sus scrofa
750
-
substrate: dimyristoyl-sn-phosphatidylcholine Naja melanoleuca
1220
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol Naja melanoleuca
3235
-
substrate: 1,2-didecanoyl-sn-glycero-3-phosphocholine Naja melanoleuca
4650
-
substrate: 1,2-dilaureoyl-sn-glycero-3-phosphocholine Naja melanoleuca
8870
-
substrate: 1,2-dinonanoyl-sn-glycero-3-phosphocholine Naja melanoleuca
9300
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol Homo sapiens
11400
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphocholine Naja melanoleuca
16000
-
substrate: 1,2-dioctanoyl-sn-glycero-3-phosphoglycol Sus scrofa

Storage Stability

Storage Stability Organism
room temperature, in presence or absence of Ca2+, pH ranging from 4.5 to 9.0, after 7 days more than 95% of the activity remains Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O
-
Homo sapiens 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
?
1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O
-
Sus scrofa 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
?
1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O
-
Naja melanoleuca 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
?
1,2-didodecanoyl-sn-glycero-3-phosphocholine + H2O
-
Homo sapiens 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
?
1,2-didodecanoyl-sn-glycero-3-phosphocholine + H2O
-
Sus scrofa 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
?
1,2-didodecanoyl-sn-glycero-3-phosphocholine + H2O
-
Naja melanoleuca 1-decanoyl-sn-glycero-3-phosphocholine + decanoate
-
?
1,2-diheptanoyl-sn-glycero-3-phophorylcholine + H2O
-
Homo sapiens 1-heptanoyl-sn-glycero-3-phophorylcholine + heptanoate
-
?
1,2-diheptanoyl-sn-glycero-3-phophorylcholine + H2O
-
Sus scrofa 1-heptanoyl-sn-glycero-3-phophorylcholine + heptanoate
-
?
1,2-diheptanoyl-sn-glycero-3-phophorylcholine + H2O
-
Naja melanoleuca 1-heptanoyl-sn-glycero-3-phophorylcholine + heptanoate
-
?
1,2-diheptanoyl-sn-glycero-3-sulfate + H2O
-
Homo sapiens 1-heptanoyl-sn-glycero-3-sulfate + heptanoate
-
?
1,2-diheptanoyl-sn-glycero-3-sulfate + H2O
-
Sus scrofa 1-heptanoyl-sn-glycero-3-sulfate + heptanoate
-
?
1,2-diheptanoyl-sn-glycero-3-sulfate + H2O
-
Naja melanoleuca 1-heptanoyl-sn-glycero-3-sulfate + heptanoate
-
?
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
Homo sapiens 1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate
-
?
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
Sus scrofa 1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate
-
?
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
Naja melanoleuca 1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate
-
?
1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O
-
Homo sapiens 1-lauroyl-sn-glycero-3-phosphocholine + laureate
-
?
1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O
-
Sus scrofa 1-lauroyl-sn-glycero-3-phosphocholine + laureate
-
?
1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O
-
Naja melanoleuca 1-lauroyl-sn-glycero-3-phosphocholine + laureate
-
?
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
Homo sapiens 1-myristoyl-phosphorylcholine + myristic acid
-
?
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
Sus scrofa 1-myristoyl-phosphorylcholine + myristic acid
-
?
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
Naja melanoleuca 1-myristoyl-phosphorylcholine + myristic acid
-
?
1,2-dinonanoyl-sn-glycero-3-phosphocholine + H2O
-
Homo sapiens 1-nonanoyl-sn-glycero-3-phosphocholine + nonanoate
-
?
1,2-dinonanoyl-sn-glycero-3-phosphocholine + H2O
-
Sus scrofa 1-nonanoyl-sn-glycero-3-phosphocholine + nonanoate
-
?
1,2-dinonanoyl-sn-glycero-3-phosphocholine + H2O
-
Naja melanoleuca 1-nonanoyl-sn-glycero-3-phosphocholine + nonanoate
-
?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
Homo sapiens 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
Sus scrofa 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
Naja melanoleuca 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
?
1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O
-
Homo sapiens 1-octanoyl-sn-glycero-3-phosphoglycol + octanoate
-
?
1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O
-
Sus scrofa 1-octanoyl-sn-glycero-3-phosphoglycol + octanoate
-
?
1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O
-
Naja melanoleuca 1-octanoyl-sn-glycero-3-phosphoglycol + octanoate
-
?
1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
Homo sapiens 1-oleoyl-sn-phosphatidylcholine + oleate
-
?
1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
Sus scrofa 1-oleoyl-sn-phosphatidylcholine + oleate
-
?
1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
Naja melanoleuca 1-oleoyl-sn-phosphatidylcholine + oleate
-
?
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
Homo sapiens 1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
?
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
Sus scrofa 1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
?
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
Naja melanoleuca 1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
?
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
Homo sapiens 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
?
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
Sus scrofa 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
?
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
Naja melanoleuca 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
?
1-acyl-2-linoleoyl-3-phosphoethanolamine + H2O
-
Homo sapiens 1-acyl-3-phosphoethanolamine + linolic acid
-
?
1-acyl-2-linoleoyl-3-phosphoethanolamine + H2O
-
Sus scrofa 1-acyl-3-phosphoethanolamine + linolic acid
-
?
1-acyl-2-linoleoyl-3-phosphoethanolamine + H2O
-
Naja melanoleuca 1-acyl-3-phosphoethanolamine + linolic acid
-
?
dioleoyl phosphatidylglycerol + H2O
-
Homo sapiens ?
-
?
dioleoyl phosphatidylglycerol + H2O
-
Sus scrofa ?
-
?
dioleoyl phosphatidylglycerol + H2O
-
Naja melanoleuca ?
-
?
phospholipids + H2O influence on platelet aggregation Homo sapiens ?
-
?
phospholipids + H2O inflammatory diseases Homo sapiens ?
-
?
phospholipids + H2O release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins Homo sapiens ?
-
?
phospholipids + H2O release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins Sus scrofa ?
-
?
rac-1,2-dihexanoyldithiopropyl-3-phosphocholine + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Homo sapiens
monomer
-
Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
after 7 days more than 95% of the activity remains, in presence or absence of Ca2+, pH ranging from 4.5 to 9.0 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Homo sapiens
8
-
-
Sus scrofa