Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.4 extracted from

  • Jung, K.M.; Kim, D.K.
    Purification and characterization of a membrane-associated 48-kilodalton phospholipase A2 in leaves of broad bean (2000), Plant Physiol., 123, 1057-1067.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin
-
 Vicia faba
Triton X-100 5fold increase of activity at 0.05% Vicia faba

General Stability

General Stability Organism
partially purified enzyme is unstable Vicia faba

Inhibitors

Inhibitors Comment Organism Structure
5,8,11,14-Eicosatetraynoic acid
-
 Vicia faba

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for Ca2+ Vicia faba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 SDS-PAGE Vicia faba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Vicia faba role in the release of linolenic acid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Vicia faba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vicia faba

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0069
-
-
 Vicia faba

Storage Stability

Storage Stability Organism
-70°C, stable, purified enzyme Vicia faba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-stearoyl-2-arachidonyl-sn-glycerol-3-phosphocholine + H2O
-
 Vicia faba 1-stearoyl-sn-glycero-3-phosphocholine + arachidonic acid
-
 ?
1-stearoyl-2-linolenoyl-sn-glycerol-3-phosphocholine + H2O preferred substrate Vicia faba ?
-
 ?
1-stearoyl-2-linoleoyl-sn-glycerol-3-phosphocholine + H2O
-
 Vicia faba 1-stearoyl-sn-glycero-3-phosphocholine + linoic acid
-
 ?
1-stearoyl-2-palmitoyl-sn-glycerol-3-phosphocholine + H2O
-
 Vicia faba 1-stearoyl-sn-glycero-3-phosphocholine + palmitic acid
-
 ?
additional information enzyme prefers phosphocholine to ethanolamine and unsaturated fatty acids to saturated fatty acids at sn-2 position of the phospholipids Vicia faba ?
-
 ?
additional information role in the release of linolenic acid Vicia faba ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Vicia faba