Any feedback?
Literature summary for 3.1.1.4 extracted from
- Identification and characterization of a phospholipase A2 from the venom of the Saw-scaled viper: Novel bactericidal and membrane damaging activities (2010), Biochimie, 92, 1854-1866.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the purified enzyme shows strong bactericidal activity against Burkholderia pseudomallei (minimum inhibitory concentration above 0.015 mg/ml) and Enterobacter aerogenes (minimum inhibitory concentration above 0.03 mg/ml) | Echis carinatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13862 | - |
x * 13862, MALDI-TOF mass spectrometry | Echis carinatus |
| 15000 | - |
x * 15000, SDS-PAGE | Echis carinatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Echis carinatus | - |
saw-scaled viper | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| C8 Sepharose column chromatography, C18 Sepharose column chromatography, and Superdex G-75 gel filtration | Echis carinatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Echis carinatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1034 | - |
crude venom, pH and temperature not specified in the publication | Echis carinatus |
| 2312 | - |
purified enzyme, pH and temperature not specified in the publication | Echis carinatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Echis carinatus | 1-acylglycerophosphocholine + fatty acid | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 15000, SDS-PAGE | Echis carinatus |
| ? | x * 13862, MALDI-TOF mass spectrometry | Echis carinatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PLA2 | - |
Echis carinatus |
| toxin I | - |
Echis carinatus |
| Tx-1 | - |
Echis carinatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the phospholipase A2 toxin I displays a heparin binding C-terminal region, which is probably responsible for the cytotoxic and bactericidal effects. Toxin I is able to induce cytotoxicity on THP-1 cells in vitro as well as lethality in BALB/c mice. Toxin II also induces mild myotoxic effects on mouse skin, but is devoid of hemolytic effects on human erythrocytes up to 0.5 mg/ml | Echis carinatus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
