Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-bromophenacyl bromide | - |
Daboia russelii | |
AIPLAI | PLA2 inhibitor isolated from the methanol extract of Azadirachta indica | Daboia russelii | |
dithiothreitol | - |
Daboia russelii | |
EDTA | - |
Daboia russelii | |
factor Xa | - |
Daboia russelii | |
iodoacetamide | - |
Daboia russelii | |
N-alpha-p-tosyl-L-lysine chloromethyl ketone | - |
Daboia russelii | |
N-tosyl-L-phenylalanyl chloromethyl ketone | - |
Daboia russelii | |
phenylmethylsulfonyl fluoride | - |
Daboia russelii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
phosphatidylcholine | pH 8.0, 37°C | Daboia russelii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28500 | - |
2 * 28500, SDS-PAGE | Daboia russelii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipids + H2O | Daboia russelii | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daboia russelii | - |
Russell's viper | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Daboia russelii |
Purification (Comment) | Organism |
---|---|
reverse phase C18-mu-Nova pack column chromatography, CM Sephadex C-50 gel filtration and DEAE Sephadex A-50 gel filtration | Daboia russelii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Daboia russelii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
crude enzyme has a specific activity of 0.00012 units/mg, after 7fold purification the enzyme has a specific activity of 0.00084 units/mg, at pH 8.0 and 37°C, one unit of activity is defined as the amount of protein which produces a decrease in 0.01 absorbance in 10 min at 740 nm | Daboia russelii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acidic PLA2 non-inhibits the factor Xa in a non-enzymatic mechanism | Daboia russelii | ? | - |
? | |
additional information | the enzyme does not show any proteolytic, ATPase and AchEase activity at a dose of 750 mM | Daboia russelii | ? | - |
? | |
phosphatidylcholine + H2O | the enzyme shows preferential hydrolysis of phosphatidylcholine | Daboia russelii | ? | - |
? | |
phospholipids + H2O | - |
Daboia russelii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28500, SDS-PAGE | Daboia russelii |
Synonyms | Comment | Organism |
---|---|---|
acidic phospholipase A2 | - |
Daboia russelii |
RVV acidic PLA2-I | - |
Daboia russelii |
RVVA-PLA2-I | - |
Daboia russelii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Daboia russelii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme inhibits the blood coagulation cascade non-enzymatically by binding with coagulation factor Xa, even in the absence of phospholipids and Ca2+ and thus slows down the blood coagulation by partially inhibiting the prothrombin activation | Daboia russelii |