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5.6.1.1: microtubule-severing ATPase

This is an abbreviated version!
For detailed information about microtubule-severing ATPase, go to the full flat file.

Word Map on EC 5.6.1.1

Reaction

n ATP + n H2O +

a microtubule
= n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers

Synonyms

adseverin, Arabidopsis thaliana katanin small subunit, AtKSS, D-spastin, DGL1, Dm-Kat60, Drosophila katanin Dm-Kat60, EC 3.6.4.3, kananin, Kat60, katanin, KATANIN 1, katanin p60, katanin p60 ATPase-containing subunit A1, katanin p60 microtubule-severing protein, KATNAL1, kinesin spindle protein, kp60-NTD, KSP, mei-1, MEI-1/MEI-2 complex, MEI-1/MEI-2 katanin complex, microtuble severing AAA ATPase spastin, microtuble-severing AAA ATPase, microtuble-severing ATPase spastin, microtubule severing protein, microtubule-severing AAA ATPase, microtubule-severing ATPase, microtubule-severing complex katanin, microtubule-severing complex MEI-1/MEI-2 katanin, microtubule-severing protein, microtubule-stimulated ATPase, MT-severing AAA ATPase, P60, p60 KAT1p, p60 katanin, P60-kananin, P60-katanin, p81-p60, Pf15, plant microtubule-severing protein, rice KTN1, scinderin, SPAS-1, spastin, spastin AAA domain, SPG4, Sug1p, vertebrate katanin

ECTree

     5 Isomerases
         5.6 Isomerases altering macromolecular conformation
             5.6.1 Enzymes altering polypeptide conformation or assembly
                5.6.1.1 microtubule-severing ATPase

Cloned

Cloned on EC 5.6.1.1 - microtubule-severing ATPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a glutathione S-transferase-tagged form of kp60-NTD expressed in Escherichia coli BL21 (DE3)
-
adaptor-p80 fragments expressed in Escherichia coli BL21(DE3), GST tagged p60-katanin expressed in Escherichia coli JM109
baculo system for pull-down assay, TNT in vitro translation kit for S35-Met-labeled recombinant protein, cloned into pEGFP or pcDNA3.1 for transfection of COS-7 cells for immuno precipitation
-
baculovirus-expressed
-
cDNA in TA cloning vector for sequencing, for expression in cultured neurons or fibroblasts in pEGFP and pCMV-Tag vector
-
cloned in pDsRed vector for transfection of chondrocytes
-
cloning of Cp60 katanin cDNA, expressed in Escherichia coli BL21(DE3)
-
coding sequence of spastin fused N-terminally to EGFP (pEGFP-N3 vector)
-
D-spastin-GFP for localization studies
-
expressed in 293T HEK cells
expressed in Escherichia coli BL21(DE3), FLAG tagged versions expressed in HEK293 cells
expressed in NIH-3T3 cells
-
expression in Escherichia coli BL21
expression in Escherichia coli BL21 (DE3)
expression of the p60N/p80C complex in Escherichiacoli BL21 (DE3) cells
-
expression of the selenomethionine-substituted (Se-MEI-1/MEI-2) katanin complex in Escherichia coli BL21
gene Katna1, cloning of the enzyme katanin p60 subunit from rat liver, recombinant expression of His-tagged protein in Escherichia coli strain BL21(DE3) pLysS
GST fusion of amino acid residues 228-616 containing the E442Q mutation (42500 Da for the 389 amino acid residues construct (amino acid residues 228-616)), expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL
-
His tagged variants and GST fusions expressed in Escherichia coli BL21 (RIL)
-
human cDNA PCR amplified
human katanin cloned and expressed in Escherichia coli
-
human spastin was cloned into the pGBK-T7 plasmid for yeast two-hybrid screening
-
into pQE30 vector, His-tag, and pGEX 4T3, GST-fusion, for expression in Escherichia coli
into the pGEX-6P-3 vector for expression in Escherichia coli BL21-CodonPlus DE3-RIPL cells
-
into the TOPO TA cloning vector, into the plasmid pPD49.26
-
isolation of cDNA clones for the p60 and p80 subunits, heterologous expression of p60 in Sf9 cell baculovirus system
-
Mei-1 and Mei-2 cloned and coexpressed in HeLa cells
-
mouse p80-CTD (residues 481-658) are cloned into a modified version of the pET-15b vector, pHisTrx2, which includes Escherichia coli thioredoxin A as a fusion protein after the His6 tag
Q9WV86; Q8BG40
overexpression of the catalytic subunit Kat60 in Drosophila S2 cells
pBSK vector for subcloning of the PCR products, pACN and pbinSRN for generating plasmids coding for fusion constructs of the target protein
-
recombinant expression of untagged short isoform of MEI-1 subunit, coexpression with MBP-TEV-tagged MEI-2 subunit, in Escherichia coli
-
SPAS-1 protein (451 amino acid reisdues) is produced as an N-terminally His6-tagged recombinant protein, expressed in Escherichia coli BL21(DE3)
-
spastin cDNA is cloned into the pGEMT easy vector, for generating a bait construct for yeast two-hybrid analysis, the excised spastin fragment is cloned into the pGBKT7 vector, for expression analysis in mammalian cells, the fragment is cloned into the pCS2-myc and pEGFP-C1 vectors
-
spastin cDNA was cloned into pGEX6p-3 for production of recombinant protein in Escherichia coli or into pEYFP-C1
-
TA-cloned for sequencing, subcloned into pFLAG-CMV-6a for transfection of HEK293 cells
the human short isoform of spastin initiating from the alternative start codon is amplified by PCR and cloned into the pET41a vector for expression in Escherichia coli BL21DE3 cells
-
the motor domain of wild-type KSP monomer, aa 1-360, is expressed in Escherichia coli BL21DE3 cells
-
three domains of AtKSS are defined, AtKSS1, aa 1-34, AtKSS2, aa 35-210, AtKSS3, the ATPase domain, aa 211-523, in addition, combinations are defined, AtKKS12 and AtKKS23, AtKSS1,2,3,12,and 23 are cloned into pGEX-4T-1 and pOL-LT plasmids