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Literature summary for 5.6.1.1 extracted from

  • Evans, K.; Keller, C.; Pavur, K.; Glasgow, K.; Conn, B.; Lauring, B.
    Interaction of two hereditary spastic paraplegia gene products, spastin and atlastin, suggests a common pathway for axonal maintenance (2006), Proc. Natl. Acad. Sci. USA, 103, 10666-10671.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.1

Application

Application Comment Organism
medicine a major fraction of autosomal dominant cases of hereditary spastic paraplegia (HSP) is caused by mutations in SPG4 encoding spastin Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
human spastin was cloned into the pGBK-T7 plasmid for yeast two-hybrid screening Homo sapiens

Protein Variants

Protein Variants Comment Organism
Delta1-132 YFP-spastin mutant, amino acids 1-132 deleted Homo sapiens
Delta116-194 YFP-spastin mutant, amino acids 116-194 deleted Homo sapiens
Delta195-227 YFP-spastin mutant, amino acids 195-227 deleted Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Homo sapiens
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant protein using a glutathione-sepharose column, removing of the GST moiety with precision protease Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
microtubule-severing AAA ATPase
-
 Homo sapiens
MT-severing AAA ATPase
-
 Homo sapiens
spastin
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 microtubule severing assay Homo sapiens