Literature summary for 5.6.1.1 extracted from

Eckert, T.; Link, S.; Le, D.T.; Sobczak, J.P.; Gieseke, A.; Richter, K.; Woehlke, G.
Subunit interactions and cooperativity in the microtubule-severing AAA ATPase spastin (2012), J. Biol. Chem., 287, 26278-26290.

Search for more literature for 5.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
His tagged variants and GST fusions expressed in Escherichia coli BL21 (RIL)	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA1-226	full severing activity	Homo sapiens
E442Q	no ATP turnover detectable, coassembled with wild type subunits	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-adenylyl-beta,gamma-imidodiphosphate	purley competitive inhibitor	Homo sapiens
ATPgammaS	mixed inhibition of ATP turnover	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.16	-	ATP	DELTA1-226, basal turnover, pH 6.8, temperature not specified in the publication	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
GSH-Sepharose affinity chromatography, ion exchange chromatography, cleavage with precission protease, GSH-Sepharose affinity chromatography and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	fragmentation of microtubles	Homo sapiens	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
hexamer	hexameric ring, allosteric interaction	Homo sapiens

Synonyms

Synonyms	Comment	Organism
microtuble-severing AAA ATPase	-	Homo sapiens
spastin	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.78	-	ATP	DELTA1-226, basal turnover, pH 6.8, temperature not specified in the publication	Homo sapiens
3.83	-	ATP	DELTA1-226, microtuble activated turnover, pH 6.8, temperature not specified in the publication	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.11	-	ATPgammaS	pH 6.8, temperature not specified in the publication	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	important for motoneuron integrity	Homo sapiens

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Release 2023.1 (January 2023)