5.6.1.1: microtubule-severing ATPase
This is an abbreviated version!
For detailed information about microtubule-severing ATPase, go to the full flat file.
Word Map on EC 5.6.1.1
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5.6.1.1
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spastic
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hereditary
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kinesins
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paraplegia
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mitotic
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cortical
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cytoskeleton
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disassembly
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centrosome
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gelsolin
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corticospinal
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atpases
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cytokine
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paraparesis
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atlastin
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microtubule-based
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actin-binding
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microtubule-binding
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escrt-iii
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antimitotic
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axonopathy
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minus-ends
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gamma-tubulin
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kinesin-related
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plus-ends
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mt-associated
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microtubule-dependent
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phragmoplasts
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midbody
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medicine
- 5.6.1.1
- spastic
- hereditary
- kinesins
- paraplegia
-
mitotic
- cortical
- cytoskeleton
-
disassembly
- centrosome
- gelsolin
-
corticospinal
- atpases
- cytokine
- paraparesis
-
atlastin
-
microtubule-based
-
actin-binding
-
microtubule-binding
-
escrt-iii
-
antimitotic
-
axonopathy
-
minus-ends
- gamma-tubulin
-
kinesin-related
-
plus-ends
-
mt-associated
-
microtubule-dependent
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phragmoplasts
- midbody
- medicine
Reaction
n ATP
+
n H2O
+
Synonyms
adseverin, Arabidopsis thaliana katanin small subunit, AtKSS, D-spastin, DGL1, Dm-Kat60, Drosophila katanin Dm-Kat60, EC 3.6.4.3, kananin, Kat60, katanin, KATANIN 1, katanin p60, katanin p60 ATPase-containing subunit A1, katanin p60 microtubule-severing protein, KATNAL1, kinesin spindle protein, kp60-NTD, KSP, mei-1, MEI-1/MEI-2 complex, MEI-1/MEI-2 katanin complex, microtuble severing AAA ATPase spastin, microtuble-severing AAA ATPase, microtuble-severing ATPase spastin, microtubule severing protein, microtubule-severing AAA ATPase, microtubule-severing ATPase, microtubule-severing complex katanin, microtubule-severing complex MEI-1/MEI-2 katanin, microtubule-severing protein, microtubule-stimulated ATPase, MT-severing AAA ATPase, P60, p60 KAT1p, p60 katanin, P60-kananin, P60-katanin, p81-p60, Pf15, plant microtubule-severing protein, rice KTN1, scinderin, SPAS-1, spastin, spastin AAA domain, SPG4, Sug1p, vertebrate katanin
ECTree
5.6.1.1 microtubule-severing ATPaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 5.6.1.1 - microtubule-severing ATPase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
ATP hydrolysis is accompanied by microtubule disassembly
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?
ATP + H2O
ADP + phosphate
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-
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?
ATP + H2O
ADP + phosphate
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recombinant Dm-Kat60 robustly severs taxol-stabilized microtubules in the presence of ATP. This reacton is inhibited when ATP is depleted with hexokinase and glucose or replaced with the non-hydrolysable ATP analogue 5'-adenylyl-beta-gamma-imidotriphosphate AMPPMP
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-
?
ATP + H2O
ADP + phosphate
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-
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?
ATP + H2O
ADP + phosphate
binding of either microtuble or C-terminal domain of adaptor-p80 stimulates the ATPase activity of p60-katanin
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-
?
ATP + H2O
ADP + phosphate
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-
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?
ATP + H2O
ADP + phosphate
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-
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-
?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
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-
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?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
assembly of female meiotic spindles requires MEI-1 and MEI-2, which constitute the microtubule-severing AAA+ ATPase Katanin
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-
?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
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-
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?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
the microtubule-interacting and trafficking (MIT) domain and adjacent linker region of the Drosophila katanin catalytic subunit Kat60 cooperate to regulate microtubule severing in two distinct ways. First, the MIT domain and linker region of Kat60 decrease its abundance by enhancing its proteasome-dependent degradation. The Drosophila katanin regulatory subunit Kat80, which is required to stabilize Kat60 in cells, conversely reduces the proteasome-dependent degradation of Kat60. Second, the MIT domain and linker region of Kat60 augment its microtubule-disassembly activity by enhancing its association with microtubules
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-
?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
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-
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?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
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-
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?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
the enzyme plays important roles in various cellular events including axon regeneration, cytokinesis, and nuclear envelope sealing after mitosis
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?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
in addition to the AAA domain, the microtubule-binding domain region of spastin is also involved in regulating ATPase activity
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?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
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katanin-dependent severing of microtubules is important for proper execution of key cellular activities including cell division, migration, and differentiation
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-
?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
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activity of katanin requires specific domains of both p60 KAT1p catalytic subunit and p80 KAT3p regulatory subunit. In addition to its activating role, p80 can also contribute to the inhibition of p60 KAT1p. Increased levels of tubulin polyglutamylation correlates with a relocalization of p60 KAT1p to glutamylated microtubules, suggesting that binding of katanin subunits to microtubules is regulated differently and that initial binding of katanin p60 KAT1p but not p80 KAT3p is stimulated by glutamylation
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-
?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
Tetrahymena thermophila CU428.2
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katanin-dependent severing of microtubules is important for proper execution of key cellular activities including cell division, migration, and differentiation
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-
?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
Tetrahymena thermophila CU428.2
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activity of katanin requires specific domains of both p60 KAT1p catalytic subunit and p80 KAT3p regulatory subunit. In addition to its activating role, p80 can also contribute to the inhibition of p60 KAT1p. Increased levels of tubulin polyglutamylation correlates with a relocalization of p60 KAT1p to glutamylated microtubules, suggesting that binding of katanin subunits to microtubules is regulated differently and that initial binding of katanin p60 KAT1p but not p80 KAT3p is stimulated by glutamylation
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-
?
ATP + H2O + microtubule
ADP + phosphate + alpha/beta tubulin heterodimers
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-
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?
GTP + H2O
GDP + phosphate
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-
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?
GTP + H2O
GDP + phosphate
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?
additional information
?
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direct role of microtubule severing in translocation of the meiotic spindle to the cortex
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?
additional information
?
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the enzyme has multiple effects on microtubules in vitro. Purified MEI-1/MEI-2 complexes preferentially sever at intersections between two microtubules and directly bundle microtubules in vitro. These activities promote parallel/antiparallel microtubule organization in meiotic spindles, MEI-1/MEI-2 complexes bundle microtubules and preferentially sever at intersections between two microtubules in vitro
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?
additional information
?
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enzyme acts on defects in microtubule lattice, promotes loss of tubulin at the defect site until the two microtubule segments are held together so weakly that mechanically unconstrained microtubules kink at the defect site
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?
additional information
?
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enzyme activity regulates the number of microtubule end in mitotic spindle, enzyme inhibition slows the rate of spindle microtubule disassembly
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?
additional information
?
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katanin p60 destabilizes microtubules at the central spindle and the midbody
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?
