Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.1.1 extracted from

  • Lohret, T.A.; Zhao, L.; Quarmby, L.M.
    Cloning of Chlamydomonas p60 katanin and localization to the site of outer doublet severing during deflagellation (1999), Cell Motil. Cytoskeleton, 43, 221-231.
    View publication on PubMed
Search for more literature for 5.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Strongylocentrotus purpuratus
cloning of Cp60 katanin cDNA, expressed in Escherichia coli BL21(DE3) Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 proteolytic product of the p60 subunit, SDS-PAGE Chlamydomonas reinhardtii
60000
-
 1 * 80000 + 1 * 60000, SDS-PAGE Chlamydomonas reinhardtii
60000
-
 1 * 80000 + 1 * 60000, SDS-PAGE Strongylocentrotus purpuratus
60100
-
 theoretical value, calculated from amino acid sequence Chlamydomonas reinhardtii
62000
-
 recombinant Cp60 Chlamydomonas reinhardtii
80000
-
 1 * 80000 + 1 * 60000, SDS-PAGE Chlamydomonas reinhardtii
80000
-
 1 * 80000 + 1 * 60000, SDS-PAGE Strongylocentrotus purpuratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Chlamydomonas reinhardtii
-
 ADP + phosphate
-
 ?
ATP + H2O Homo sapiens
-
 ADP + phosphate
-
 ?
ATP + H2O Xenopus laevis
-
 ADP + phosphate
-
 ?
ATP + H2O Strongylocentrotus purpuratus
-
 ADP + phosphate
-
 ?
GTP + H2O Chlamydomonas reinhardtii
-
 GDP + phosphate
-
 ?
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?
GTP + H2O Xenopus laevis
-
 GDP + phosphate
-
 ?
GTP + H2O Strongylocentrotus purpuratus
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Homo sapiens
-
 human
-
Homo sapiens
-
 human cell lines MSU1.1 and HeLa
-
Strongylocentrotus purpuratus
-
 sea urchin
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Strongylocentrotus purpuratus
p60, 60 kDa catalytic subunit Chlamydomonas reinhardtii

Reaction

Reaction Comment Organism Reaction ID
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome Chlamydomonas reinhardtii
a
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome Homo sapiens
a
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome Xenopus laevis
a
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome Strongylocentrotus purpuratus
a

Source Tissue

Source Tissue Comment Organism Textmining
oocyte
-
 Xenopus laevis
-
oocyte
-
 Strongylocentrotus purpuratus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Chlamydomonas reinhardtii ADP + phosphate
-
 ?
ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?
ATP + H2O
-
 Xenopus laevis ADP + phosphate
-
 ?
ATP + H2O
-
 Strongylocentrotus purpuratus ADP + phosphate
-
 ?
GTP + H2O
-
 Chlamydomonas reinhardtii GDP + phosphate
-
 ?
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?
GTP + H2O
-
 Xenopus laevis GDP + phosphate
-
 ?
GTP + H2O
-
 Strongylocentrotus purpuratus GDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Xenopus laevis
dimer 1 * 80000 + 1 * 60000, SDS-PAGE Chlamydomonas reinhardtii
dimer 1 * 80000 + 1 * 60000, SDS-PAGE Strongylocentrotus purpuratus