Activating Compound | Comment | Organism | Structure |
---|---|---|---|
microtubule | - |
Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
three domains of AtKSS are defined, AtKSS1, aa 1-34, AtKSS2, aa 35-210, AtKSS3, the ATPase domain, aa 211-523, in addition, combinations are defined, AtKKS12 and AtKKS23, AtKSS1,2,3,12,and 23 are cloned into pGEX-4T-1 and pOL-LT plasmids | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
GST-AtKSS1, determined by SDS-PAGE | Arabidopsis thaliana |
46000 | - |
GST-AtKSS2, determined by SDS-PAGE | Arabidopsis thaliana |
50000 | - |
GST-AtKSS12, determined by SDS-PAGE | Arabidopsis thaliana |
60000 | - |
catalytic subunit p60 | Arabidopsis thaliana |
62000 | - |
GST-AtKSS3, determined by SDS-PAGE | Arabidopsis thaliana |
66000 | - |
GST-AtKSS13, determined by SDS-PAGE | Arabidopsis thaliana |
80000 | - |
regulatory subunit p80 | Arabidopsis thaliana |
82000 | - |
GST-AtKSS23, determined by SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Arabidopsis thaliana | - |
ADP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SEX2 | - |
- |
Purification (Comment) | Organism |
---|---|
using a glutathione Sepharose 4B column | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Arabidopsis thaliana | ADP + phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
heterodimer | katanin is a heterodimer composed of a catalytic subunit that hydrolyses ATP to sever microtubules, and a regulatory subunit involved in subcellular targeting | Arabidopsis thaliana |
More | data suggest that AtKSS oligomerizes into a ring-shaped oligomer, and that the microtubule-binding domain is involved in the oligomerization process, the addition of two trimers to form a hexamer is proposed | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtKSS | katanin catalytic subunit, microtubule-stimulated ATPase activity | Arabidopsis thaliana |
katanin | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
ATPase activity assay | Arabidopsis thaliana |