Cloned (Comment) | Organism |
---|---|
human cDNA PCR amplified | Homo sapiens |
isolation of cDNA clones for the p60 and p80 subunits, heterologous expression of p60 in Sf9 cell baculovirus system | Strongylocentrotus purpuratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
centrosome | - |
Xenopus laevis | 5813 | - |
centrosome | - |
Strongylocentrotus purpuratus | 5813 | - |
centrosome | - |
Homo sapiens | 5813 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 80000 + 1 * 60000, SDS-PAGE | Chlamydomonas reinhardtii |
60000 | - |
1 * 80000 + 1 * 60000, SDS-PAGE | Strongylocentrotus purpuratus |
80000 | - |
1 * 80000 + 1 * 60000, SDS-PAGE | Chlamydomonas reinhardtii |
80000 | - |
1 * 80000 + 1 * 60000, SDS-PAGE | Strongylocentrotus purpuratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Gallus gallus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Chlamydomonas reinhardtii | - |
ADP + phosphate | - |
? | |
ATP + H2O | Mus musculus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Xenopus laevis | - |
ADP + phosphate | - |
? | |
ATP + H2O | Caenorhabditis elegans | - |
ADP + phosphate | - |
? | |
ATP + H2O | Strongylocentrotus purpuratus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
GTP + H2O | Gallus gallus | - |
GDP + phosphate | - |
? | |
GTP + H2O | Chlamydomonas reinhardtii | - |
GDP + phosphate | - |
? | |
GTP + H2O | Mus musculus | - |
GDP + phosphate | - |
? | |
GTP + H2O | Xenopus laevis | - |
GDP + phosphate | - |
? | |
GTP + H2O | Caenorhabditis elegans | - |
GDP + phosphate | - |
? | |
GTP + H2O | Strongylocentrotus purpuratus | - |
GDP + phosphate | - |
? | |
GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Chlamydomonas reinhardtii | - |
- |
- |
Gallus gallus | - |
chicken | - |
Homo sapiens | O75449 | human | - |
Homo sapiens | O75449 | human cell lines MSU1.1 and HeLa | - |
Mus musculus | - |
mouse, neuroblastoma | - |
Strongylocentrotus purpuratus | - |
sea urchin | - |
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Xenopus laevis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Gallus gallus | |
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Chlamydomonas reinhardtii | |
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Mus musculus | |
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Xenopus laevis | |
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Caenorhabditis elegans | |
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Strongylocentrotus purpuratus | |
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Homo sapiens | - |
egg | - |
Xenopus laevis | - |
egg | - |
Strongylocentrotus purpuratus | - |
embryo | - |
Strongylocentrotus purpuratus | - |
epithelium | - |
Homo sapiens | - |
fibroblast | - |
Homo sapiens | - |
neuron | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Gallus gallus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Chlamydomonas reinhardtii | ADP + phosphate | - |
? | |
ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Xenopus laevis | ADP + phosphate | - |
? | |
ATP + H2O | - |
Caenorhabditis elegans | ADP + phosphate | - |
? | |
ATP + H2O | - |
Strongylocentrotus purpuratus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
GTP + H2O | - |
Gallus gallus | GDP + phosphate | - |
? | |
GTP + H2O | - |
Chlamydomonas reinhardtii | GDP + phosphate | - |
? | |
GTP + H2O | - |
Mus musculus | GDP + phosphate | - |
? | |
GTP + H2O | - |
Xenopus laevis | GDP + phosphate | - |
? | |
GTP + H2O | - |
Caenorhabditis elegans | GDP + phosphate | - |
? | |
GTP + H2O | - |
Strongylocentrotus purpuratus | GDP + phosphate | - |
? | |
GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Xenopus laevis |
dimer | - |
Caenorhabditis elegans |
dimer | 1 * 80000 + 1 * 60000, SDS-PAGE | Chlamydomonas reinhardtii |
dimer | 1 * 80000 + 1 * 60000, SDS-PAGE | Strongylocentrotus purpuratus |
Synonyms | Comment | Organism |
---|---|---|
mei-1 | - |
Caenorhabditis elegans |
vertebrate katanin | - |
Homo sapiens |