Cloned (Comment) | Organism |
---|---|
baculovirus-expressed | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell cortex | Dm-Kat60 concentrates at the cell cortex of S2 Drosophila cells during interphase, where it suppresses the polymerization of microtubule plus-ends | Drosophila melanogaster | 5938 | - |
additional information | Dm-Kat60 also generally co-localizes with cortical actin. Cortical localization od Dm-Kat60 occurs indepently of microtubules, as it persists following microtubule depolymerization with colchicine | Drosophila melanogaster | - |
- |
additional information | Dm-Kat60 also localizes at the leading edge of migratory D17 Drosophila cells and negatively regulates multiple parameters of their motility | Drosophila melanogaster | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
baculovirus-expressed, recombinant DM-Kat60 | Drosophila melanogaster |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | Dm-Kat60 severs and depolymerizes microtubules from their ends | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Drosophila D17 cell | - |
Drosophila melanogaster | - |
SCHNEIDER-2 cell | S2 cell | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | recombinant Dm-Kat60 robustly severs taxol-stabilized microtubules in the presence of ATP. This reacton is inhibited when ATP is depleted with hexokinase and glucose or replaced with the non-hydrolysable ATP analogue 5'-adenylyl-beta-gamma-imidotriphosphate AMPPMP | Drosophila melanogaster | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dm-Kat60 | - |
Drosophila melanogaster |
Drosophila katanin Dm-Kat60 | - |
Drosophila melanogaster |
katanin | - |
Drosophila melanogaster |