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Literature summary for 5.6.1.1 extracted from

  • McNally, K.; Berg, E.; Cortes, D.B.; Hernandez, V.; Mains, P.E.; McNally, F.J.
    Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro (2014), Mol. Biol. Cell, 25, 1037-1049.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of untagged short isoform of MEI-1 subunit, coexpression with MBP-TEV-tagged MEI-2 subunit, in Escherichia coli Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Caenorhabditis elegans 5737
-
microtubule
-
 Caenorhabditis elegans 5874
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Caenorhabditis elegans
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant untagged short isoform of MEI-1 subunit and MBP-TEV-tagged MEI-2 subunit from Escherichia coli by amylose affinity chromatography, eluted with TEV protease Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Caenorhabditis elegans ADP + phosphate
-
 ?
additional information the enzyme has multiple effects on microtubules in vitro. Purified MEI-1/MEI-2 complexes preferentially sever at intersections be­tween two microtubules and directly bundle microtubules in vitro. These activities promote parallel/antiparallel microtubule organization in meiotic spindles, MEI-1/MEI-2 complexes bundle microtubules and preferentially sever at intersections between two microtubules in vitro Caenorhabditis elegans ?
-
 ?

Synonyms

Synonyms Comment Organism
katanin
-
 Caenorhabditis elegans

General Information

General Information Comment Organism
malfunction rapid inactivation of katanin results in complete loss of ASPM-1 positive spindle poles. Strong loss-of-function mutants assemble apolar intersecting microtubule arrays, whereas weaker mutants assemble bipolar meiotic spindles that are longer than wild-type. Microtubule disassembly in enzyme point mutants, overview. Progressive loss of katanin leads to a progressive loss in the organization of pole-to-pole microtubule bundles, phenotypes Caenorhabditis elegans
physiological function katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo. Assembly of Caenorhabditis elegans female meiotic spindles requires both MEI-1 and MEI-2 subunits of the microtubule-severing ATPase katanin. A linear relationship between in vitro microtubule disassembly rates and in vivo spindle length exists Caenorhabditis elegans