Application | Comment | Organism |
---|---|---|
medicine | spastin is frequently mutated in hereditary spastic paraplegia | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
the human short isoform of spastin initiating from the alternative start codon is amplified by PCR and cloned into the pET41a vector for expression in Escherichia coli BL21DE3 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E442Q | missense mutation | Homo sapiens |
R499C | missense mutation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.497 | - |
ATP | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | short isoform, lacks putative transmembrane motif | Homo sapiens | 5737 | - |
nucleus | short isoform, lacks putative transmembrane motif | Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
monomer, GST-spastin, determined by gel filtration analysis and Western blotting | Homo sapiens |
350000 | - |
hexamer, GST-free spastin, determined by gel filtration analysis and Western blotting | Homo sapiens |
530000 | - |
hexamer, GST-spastin, determined by gel filtration analysis and Western blotting | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a glutathione-agarose column, the glutathione-S-transferase moiety is cleaved with thrombin | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
spastin | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
ATPase activity assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
ATPase activity assay | Homo sapiens |